目的建立车前子的HPLC特征图谱。方法采用HPLC法测定,使用Agilent Eclipse XDB-C18色谱柱(250mm×4.6mm,5μm),以甲醇-0.1%甲酸水溶液为流动相,梯度洗脱,流速1.0 m L·min-1;检测波长250nm,柱温为30℃。对10个批次车前子检测,...目的建立车前子的HPLC特征图谱。方法采用HPLC法测定,使用Agilent Eclipse XDB-C18色谱柱(250mm×4.6mm,5μm),以甲醇-0.1%甲酸水溶液为流动相,梯度洗脱,流速1.0 m L·min-1;检测波长250nm,柱温为30℃。对10个批次车前子检测,采用国家药典委员会中药色谱指纹图谱相似度评价系统软件(2004A版)进行数据处理分析。并用HPLC-MS技术对共有峰进行了初步归属并比较了车前子与平车前子HPLC图谱的差异。结果建立了以3个共有峰为指标成分的车前子药材的HPLC特征图谱,通过MS定性分析结合对照品比对,确定共有峰分别为京尼平苷酸、毛蕊花糖苷和异毛蕊花糖苷,车前子与平车前子的HPLC图谱差异明显。结论该方法经过方法学验证,可用于车前子的质量控制。展开更多
Poliumoside is representative of phenylethanoid glycosides, which are widely found in many plants. Poliumoside is also regarded as the main active component of Callicarpa kwangtungensis Chun(CK), though its oral bioav...Poliumoside is representative of phenylethanoid glycosides, which are widely found in many plants. Poliumoside is also regarded as the main active component of Callicarpa kwangtungensis Chun(CK), though its oral bioavailability in rat is extremely low(0.69%) and its in vivo and in vitro metabolism has not yet been systematically investigated. In the present study, an ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry(UPLC/Q-TOF-MS) method was employed to identify the metabolites and investigate the metabolic pathways of poliumoside in rat after oral administration 1.5 g·kg–1 of poliumoside. As a result, a total of 34 metabolites(30 from urine, 17 from plasma, and 4 from bile) and 9 possible metabolic pathways(rearrangment, reduction, hydration, hydrolyzation, dehydration, methylation, hydroxylation, acetylation, and sulfation) were proposed in vivo. The main metabolite, acteoside, was quantified after incubated with rat intestinal bacteria in vitro. In conclusion, the present study systematically explored the metabolites of poliumoside in vivo and in vitro, proposing metabolic pathways that may be significant for further metabolic studies of poliumoside.展开更多
文摘目的建立车前子的HPLC特征图谱。方法采用HPLC法测定,使用Agilent Eclipse XDB-C18色谱柱(250mm×4.6mm,5μm),以甲醇-0.1%甲酸水溶液为流动相,梯度洗脱,流速1.0 m L·min-1;检测波长250nm,柱温为30℃。对10个批次车前子检测,采用国家药典委员会中药色谱指纹图谱相似度评价系统软件(2004A版)进行数据处理分析。并用HPLC-MS技术对共有峰进行了初步归属并比较了车前子与平车前子HPLC图谱的差异。结果建立了以3个共有峰为指标成分的车前子药材的HPLC特征图谱,通过MS定性分析结合对照品比对,确定共有峰分别为京尼平苷酸、毛蕊花糖苷和异毛蕊花糖苷,车前子与平车前子的HPLC图谱差异明显。结论该方法经过方法学验证,可用于车前子的质量控制。
文摘Poliumoside is representative of phenylethanoid glycosides, which are widely found in many plants. Poliumoside is also regarded as the main active component of Callicarpa kwangtungensis Chun(CK), though its oral bioavailability in rat is extremely low(0.69%) and its in vivo and in vitro metabolism has not yet been systematically investigated. In the present study, an ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry(UPLC/Q-TOF-MS) method was employed to identify the metabolites and investigate the metabolic pathways of poliumoside in rat after oral administration 1.5 g·kg–1 of poliumoside. As a result, a total of 34 metabolites(30 from urine, 17 from plasma, and 4 from bile) and 9 possible metabolic pathways(rearrangment, reduction, hydration, hydrolyzation, dehydration, methylation, hydroxylation, acetylation, and sulfation) were proposed in vivo. The main metabolite, acteoside, was quantified after incubated with rat intestinal bacteria in vitro. In conclusion, the present study systematically explored the metabolites of poliumoside in vivo and in vitro, proposing metabolic pathways that may be significant for further metabolic studies of poliumoside.
