To improve the prediction accuracy of chaotic time series and reconstruct a more reasonable phase space structure of the prediction network,we propose a convolutional neural network-long short-term memory(CNN-LSTM)pre...To improve the prediction accuracy of chaotic time series and reconstruct a more reasonable phase space structure of the prediction network,we propose a convolutional neural network-long short-term memory(CNN-LSTM)prediction model based on the incremental attention mechanism.Firstly,a traversal search is conducted through the traversal layer for finite parameters in the phase space.Then,an incremental attention layer is utilized for parameter judgment based on the dimension weight criteria(DWC).The phase space parameters that best meet DWC are selected and fed into the input layer.Finally,the constructed CNN-LSTM network extracts spatio-temporal features and provides the final prediction results.The model is verified using Logistic,Lorenz,and sunspot chaotic time series,and the performance is compared from the two dimensions of prediction accuracy and network phase space structure.Additionally,the CNN-LSTM network based on incremental attention is compared with long short-term memory(LSTM),convolutional neural network(CNN),recurrent neural network(RNN),and support vector regression(SVR)for prediction accuracy.The experiment results indicate that the proposed composite network model possesses enhanced capability in extracting temporal features and achieves higher prediction accuracy.Also,the algorithm to estimate the phase space parameter is compared with the traditional CAO,false nearest neighbor,and C-C,three typical methods for determining the chaotic phase space parameters.The experiments reveal that the phase space parameter estimation algorithm based on the incremental attention mechanism is superior in prediction accuracy compared with the traditional phase space reconstruction method in five networks,including CNN-LSTM,LSTM,CNN,RNN,and SVR.展开更多
Specific fluorophore was introduced into ionic liquid based on its tunability,thus a kind of novel fluorescent ionic liquid probe[P66614][HQS]was designed,synthesized and characterized.Compared with non-fluorescent HQ...Specific fluorophore was introduced into ionic liquid based on its tunability,thus a kind of novel fluorescent ionic liquid probe[P66614][HQS]was designed,synthesized and characterized.Compared with non-fluorescent HQS,ionic liquid[P66614][HQS]emitted a certain amount of fluorescence,which could be attributed to the well-delocalized frontier orbitals and its charge transfer character,as demonstrated by quantum chemical calculation.Considering the interaction of[P66614][HQS]with metal ions,the application for detecting specific substance as a chemical sensor,such as Al3+was investigated.Compared with the traditional probe HQS,significant improvements in Al^3+detecting was achieved by[P66614][HQS]with stronger binding ability,better sensitivity and selectivity.The better performance of[P66614][HQS]was contributed to the changed charge distribution,leading to the stronger binding interaction.We believe that this new fluorescent ionic liquid exhibited unique properties in detecting Al^3+in aqueous solution,which would broaden the application of ionic liquids.展开更多
An interesting phenomenon was found that fluorophore was introduced into ionic liquid(IL)to magnify fluorescence signal via the thermally activated delayed fluorescence process;thus up to 15 fold enhancements were ach...An interesting phenomenon was found that fluorophore was introduced into ionic liquid(IL)to magnify fluorescence signal via the thermally activated delayed fluorescence process;thus up to 15 fold enhancements were achieved.Hence,we reported a succinct enhanced luminescence strategy to reduce single-triplet energy split by the tunability of ILs.This strategy could be extended to more kinds of ILs by the virtue of a preliminary DFT calculation screening.Moreover,the optical feature of IL sensor made it a promising candidate as a gas fluorescence sensor.展开更多
1-deoxy-D-xylulose-5-phosphate synthase(DXS)catalyzes the first and rate-limiting step of the plastidic 2-C-methyl-D-derythritol-4-phosphate(MEP)pathway which regulates the synthesis of terpenoids,such as gibberellins...1-deoxy-D-xylulose-5-phosphate synthase(DXS)catalyzes the first and rate-limiting step of the plastidic 2-C-methyl-D-derythritol-4-phosphate(MEP)pathway which regulates the synthesis of terpenoids,such as gibberellins,abscisic acid,and chlorophyll.The objective of this study was to determine the functional role of PpDXS1 in plant growth and development in Kentucky bluegrass(Poa pratensis L.).The PpDXS1 gene has a 2139 bp open reading frame that encodes a polypeptide of 712 amino acids with a calculated molecular weight of 76.7 kDa.PpDXS1 gene expression was the highest in leaves.Moreover,the PpDXS1 promoter contained several hormone response elements and gene expression was induced by exogenous treatment with gibberellin,abscisic acid,jasmonate,and pathogen infection.Functional analysis indicated that underexpression of PpDXS1 gene in Poa pratensis decreased plant height and endogenous gibberellin and indole acetic acid production,but promoted abscisic acid accumulation.Furthermore,transcriptome analysis and qRT-PCR results showed that the expression levels of related genes involved in the phytohormone biosynthesis and signal transduction were differentially regulated by PpDXS1 in transgenic Poa pratensis.Overall,these results indicated that PpDXS1 has strong effects on plant height and accumulation of phytohormones,and provided a preliminary understanding of molecular characterization,expression and function of PpDXS1 in Poa pratensis.展开更多
Li-metal batteries(LMBs)regain research prominence owing to the ever-increasing high-energy requirements.Commercially available carbonate electrolytes exhibit unfavourable parasitic reactions with Limetal anode(LMA),l...Li-metal batteries(LMBs)regain research prominence owing to the ever-increasing high-energy requirements.Commercially available carbonate electrolytes exhibit unfavourable parasitic reactions with Limetal anode(LMA),leading to the formation of unstable solid electrolyte interphase(SEI)and the breed of Li dendrites/dead Li.Significantly,lithium nitrate(LiNO_(3)),an excellent film-forming additive,proves crucial to construct a robust Li_(3)N/Li_(2)O/Li_(x)NO_(y)-rich SEI after combining with ether-based electrolytes.Thus,the given challenge leads to natural ideas which suggest the incorporation of LiNO_(3) into commercial carbonate for practical LMBs.Regrettably,LiNO_(3) demonstrates limited solubility(~800 ppm)in commercial carbonate electrolytes.Thence,developing stable SEI and dendrite-free LMA with the incorporation of LiNO_(3) into carbonate electrolytes is an efficacious strategy to realize robust LMBs via a scalable and cost-effective route.Therefore,this review unravels the grievances between LMA,LiNO_(3)and carbonate electrolytes,and enables a comprehensive analysis of LMA stabilizing mechanism with LiNO_(3),dissolution principle of LiNO_(3) in carbonate electrolytes,and LiNO_(3) introduction strategies.This review converges attention on a point that the LiNO_(3)-introduction into commercial carbonate electrolytes is an imperious choice to realize practical LMBs with commercial 4 V layered cathode.展开更多
Accurately recognizing facial expressions is essential for effective social interactions.Non-human primates(NHPs)are widely used in the study of the neural mechanisms underpinning facial expression processing,yet it r...Accurately recognizing facial expressions is essential for effective social interactions.Non-human primates(NHPs)are widely used in the study of the neural mechanisms underpinning facial expression processing,yet it remains unclear how well monkeys can recognize the facial expressions of other species such as humans.In this study,we systematically investigated how monkeys process the facial expressions of conspecifics and humans using eye-tracking technology and sophisticated behavioral tasks,namely the temporal discrimination task(TDT)and face scan task(FST).We found that monkeys showed prolonged subjective time perception in response to Negative facial expressions in monkeys while showing longer reaction time to Negative facial expressions in humans.Monkey faces also reliably induced divergent pupil contraction in response to different expressions,while human faces and scrambled monkey faces did not.Furthermore,viewing patterns in the FST indicated that monkeys only showed bias toward emotional expressions upon observing monkey faces.Finally,masking the eye region marginally decreased the viewing duration for monkey faces but not for human faces.By probing facial expression processing in monkeys,our study demonstrates that monkeys are more sensitive to the facial expressions of conspecifics than those of humans,thus shedding new light on inter-species communication through facial expressions between NHPs and humans.展开更多
AIM:To characterize spectral-domain optical coherence tomography(SD-OCT)features of chorioretinal folds in orbital mass imaged using enhanced depth imaging(EDI).METHODS:Prospective observational case-control study was...AIM:To characterize spectral-domain optical coherence tomography(SD-OCT)features of chorioretinal folds in orbital mass imaged using enhanced depth imaging(EDI).METHODS:Prospective observational case-control study was conducted in 20 eyes of 20 patients,the uninvolved eye served as a control.All the patients underwent clinical fundus photography,computed tomography,EDI SDOCT imaging before and after surgery.Two patients with cavernous hemangiomas underwent intratumoral injection of bleomycin A5;the remaining patients underwent tumor excision.Patients were followed 1 to 14mo following surgery(average follow up,5.8mo).RESULTS:Visual acuity prior to surgery ranged from 20/20 to 20/200.Following surgery,5 patients’visual acuity remained unchanged while the remaining 15 patients had a mean letter improvement of 10(range 4 to 26 letters).Photoreceptor inner/outer segment defects were found in 10 of 15 patients prior to surgery.Following surgical excision,photoreceptor inner/outer segment defects fully resolved in 8 of these 10 patients.CONCLUSION:Persistence of photoreceptor inner/outer segment defects caused by compression of the globe by an orbital mass can be associated with reduced visual prognosis.Our findings suggest that photoreceptor inner/outer segment defects on EDI SD-OCT could be an indicator for immediate surgical excision of an orbital mass causing choroidal compression.展开更多
AIM:To illustrate clinicopathological features of orbital non-rhabdomyosarcoma soft tissue sarcoma(NRSTS),and to compare the treatment outcome between postoperative radiotherapy(RT) and chemotherapy in a retrospective...AIM:To illustrate clinicopathological features of orbital non-rhabdomyosarcoma soft tissue sarcoma(NRSTS),and to compare the treatment outcome between postoperative radiotherapy(RT) and chemotherapy in a retrospective analysis nearly 20y.METHODS:A retrospective cohort study of 56 patients with orbital NRSTS were reviewed,34 of whom received postoperative RT,and 22 received postoperative chemotherapy.The clinicopathological features,local recurrence,metastases,and survival data were recorded.Survival analysis was performed using the Kaplan-Meier method.RESULTS:During follow-up(111.8mo,ranged 8-233mo) for 56 patients,19 patients of them developed local recurrence,and 7 patients developed distant metastases.Fifteen patients died during follow-up period.Overall survival rates considering the whole study group was 78.57% at 5y,and 72.16% at 10y after the initial diagnosis.Compared with chemotherapy,RT was associated with lower risk of local recurrence [hazard ratio for RT vs chemotherapy,0.263,95% confidence interval(CI),0.095-0.728,P=0.0015];with lower risk of distant metastasis(hazard ratio for RT vs chemotherapy,0.073,95%CI,0.015-0.364,P=0.0014);and with lower risk of death from disease(hazard ratio for RT vs chemotherapy,0.066,95%CI,0.022-0.200,P<0.0001).The 5-year survival rate in RT group was 97.06% compared to 50% in chemotherapy group.CONCLUSION:In patients with orbital NRSTS,postoperative RT provides better control of local recurrence,distant metastasis,and death from disease than chemotherapy.RT is the more preferrable adjuvant therapy compared to chemotherapy possibly.展开更多
通过分析临床医学本科生耳鼻喉头颈外科学习中存在的教学困境,对教学质量满意度的影响因素进行分析,提出了一套创新的病例讨论教学法(case based study,CBS)结合传统教学方法(lecture based learning,LBL),即以病例讨论和理论知识为中...通过分析临床医学本科生耳鼻喉头颈外科学习中存在的教学困境,对教学质量满意度的影响因素进行分析,提出了一套创新的病例讨论教学法(case based study,CBS)结合传统教学方法(lecture based learning,LBL),即以病例讨论和理论知识为中心的临床教学法能充分发挥本科生的主现能动性,是提高本科生理论知识和实践操作等综合素质的有效途径。在传统授课中加入病例讨论教学,能使学生更好地掌握耳鼻咽喉头颈外科学的知识。在传统教学模式上的创新,可改善传统教学模式中枯燥、平述式教学模式,创新教学模式下有助于整体耳鼻喉科临床教学水平的提高。展开更多
The motor relearning program can significantly improve various functional disturbance induced by ischemic cerebrovascular diseases. However, its mechanism of action remains poorly understood. In injured brain tissues,...The motor relearning program can significantly improve various functional disturbance induced by ischemic cerebrovascular diseases. However, its mechanism of action remains poorly understood. In injured brain tissues, glial fibrillary acidic protein and neurofilament protein changes can reflect the condition of injured neurons and astrocytes, while vascular endothelial growth factor and basic fibroblast growth factor changes can indicate angiogenesis. In the present study, we induced ischemic brain injury in the rhesus macaque by electrocoagulation of the M1 segment of the right middle cerebral artery. The motor relearning program was conducted for 60 days from the third day after model establishment. Immunohistochemistry and single-photon emission CT showed that the numbers of glial fibrillary acidic protein-, neurofilament protein-, vascular endothelial growth factor- and basic fibroblast growth factor-positive cells were significantly increased in the infarcted side compared with the contralateral hemisphere following the motor relearning program. Moreover, cerebral blood flow in the infarcted side was significantly improved. The clinical rating scale for stroke was used to assess neurological function changes in the rhesus macaque following the motor relearning program. Results showed that motor function was improved, and problems with consciousness, self-care ability and balance function were significantly ameliorated. These findings indicate that the motor relearning program significantly promoted neuronal regeneration, repair and angiogenesis in the surroundings of the infarcted hemisphere, and improve neurological function in the rhesus macaque following brain ischemia.展开更多
文摘To improve the prediction accuracy of chaotic time series and reconstruct a more reasonable phase space structure of the prediction network,we propose a convolutional neural network-long short-term memory(CNN-LSTM)prediction model based on the incremental attention mechanism.Firstly,a traversal search is conducted through the traversal layer for finite parameters in the phase space.Then,an incremental attention layer is utilized for parameter judgment based on the dimension weight criteria(DWC).The phase space parameters that best meet DWC are selected and fed into the input layer.Finally,the constructed CNN-LSTM network extracts spatio-temporal features and provides the final prediction results.The model is verified using Logistic,Lorenz,and sunspot chaotic time series,and the performance is compared from the two dimensions of prediction accuracy and network phase space structure.Additionally,the CNN-LSTM network based on incremental attention is compared with long short-term memory(LSTM),convolutional neural network(CNN),recurrent neural network(RNN),and support vector regression(SVR)for prediction accuracy.The experiment results indicate that the proposed composite network model possesses enhanced capability in extracting temporal features and achieves higher prediction accuracy.Also,the algorithm to estimate the phase space parameter is compared with the traditional CAO,false nearest neighbor,and C-C,three typical methods for determining the chaotic phase space parameters.The experiments reveal that the phase space parameter estimation algorithm based on the incremental attention mechanism is superior in prediction accuracy compared with the traditional phase space reconstruction method in five networks,including CNN-LSTM,LSTM,CNN,RNN,and SVR.
基金We acknowledge the support of the National Key Basic Research Program of China(2015CB251401)the Natural Science Foundation of China(21776239)+1 种基金Zhejiang Provincial Natural Science Foundation of China(LZ17B060001)the Fundamental Research Funds of the Central Universities,the instrument support of professor Peng Xiaogang,professor Jin Yizheng and associate professor Qin Haiyan.
文摘Specific fluorophore was introduced into ionic liquid based on its tunability,thus a kind of novel fluorescent ionic liquid probe[P66614][HQS]was designed,synthesized and characterized.Compared with non-fluorescent HQS,ionic liquid[P66614][HQS]emitted a certain amount of fluorescence,which could be attributed to the well-delocalized frontier orbitals and its charge transfer character,as demonstrated by quantum chemical calculation.Considering the interaction of[P66614][HQS]with metal ions,the application for detecting specific substance as a chemical sensor,such as Al3+was investigated.Compared with the traditional probe HQS,significant improvements in Al^3+detecting was achieved by[P66614][HQS]with stronger binding ability,better sensitivity and selectivity.The better performance of[P66614][HQS]was contributed to the changed charge distribution,leading to the stronger binding interaction.We believe that this new fluorescent ionic liquid exhibited unique properties in detecting Al^3+in aqueous solution,which would broaden the application of ionic liquids.
基金the support of the Natural Science Foundation of China(21776239)Zhejiang Provincial Natural Science Foundation of China(LZ17B060001)+1 种基金the Fundamental Research Funds of the Central UniversitiesResearch Initiation Fund of Zhejiang University of Technology(2020101005929)。
文摘An interesting phenomenon was found that fluorophore was introduced into ionic liquid(IL)to magnify fluorescence signal via the thermally activated delayed fluorescence process;thus up to 15 fold enhancements were achieved.Hence,we reported a succinct enhanced luminescence strategy to reduce single-triplet energy split by the tunability of ILs.This strategy could be extended to more kinds of ILs by the virtue of a preliminary DFT calculation screening.Moreover,the optical feature of IL sensor made it a promising candidate as a gas fluorescence sensor.
基金supported by the National Natural Science Foundation of China(No.31302016).
文摘1-deoxy-D-xylulose-5-phosphate synthase(DXS)catalyzes the first and rate-limiting step of the plastidic 2-C-methyl-D-derythritol-4-phosphate(MEP)pathway which regulates the synthesis of terpenoids,such as gibberellins,abscisic acid,and chlorophyll.The objective of this study was to determine the functional role of PpDXS1 in plant growth and development in Kentucky bluegrass(Poa pratensis L.).The PpDXS1 gene has a 2139 bp open reading frame that encodes a polypeptide of 712 amino acids with a calculated molecular weight of 76.7 kDa.PpDXS1 gene expression was the highest in leaves.Moreover,the PpDXS1 promoter contained several hormone response elements and gene expression was induced by exogenous treatment with gibberellin,abscisic acid,jasmonate,and pathogen infection.Functional analysis indicated that underexpression of PpDXS1 gene in Poa pratensis decreased plant height and endogenous gibberellin and indole acetic acid production,but promoted abscisic acid accumulation.Furthermore,transcriptome analysis and qRT-PCR results showed that the expression levels of related genes involved in the phytohormone biosynthesis and signal transduction were differentially regulated by PpDXS1 in transgenic Poa pratensis.Overall,these results indicated that PpDXS1 has strong effects on plant height and accumulation of phytohormones,and provided a preliminary understanding of molecular characterization,expression and function of PpDXS1 in Poa pratensis.
基金the support by the Leading Innovative and Entrepreneur Team Introduction Program of Zhejiang(2019R01006)the National Natural Science Foundation of China(NSFC:12205252)+3 种基金A Project Supported by Scientific Research Fund of Zhejiang Provincial Education Department(Y202250795)the Research Funds of Institute of Zhejiang University-Quzhou,the Basic Public Welfare Research Special Project of Zhejiang Province(LZY22B040001)the Science and Technology Project of Quzhou Research Institute,Zhejiang University(IZQ2021KJ2032)the Independent Scientific Research Project of Quzhou Research Institute,Zhejiang University(IZQ2021RCZX007)。
文摘Li-metal batteries(LMBs)regain research prominence owing to the ever-increasing high-energy requirements.Commercially available carbonate electrolytes exhibit unfavourable parasitic reactions with Limetal anode(LMA),leading to the formation of unstable solid electrolyte interphase(SEI)and the breed of Li dendrites/dead Li.Significantly,lithium nitrate(LiNO_(3)),an excellent film-forming additive,proves crucial to construct a robust Li_(3)N/Li_(2)O/Li_(x)NO_(y)-rich SEI after combining with ether-based electrolytes.Thus,the given challenge leads to natural ideas which suggest the incorporation of LiNO_(3) into commercial carbonate for practical LMBs.Regrettably,LiNO_(3) demonstrates limited solubility(~800 ppm)in commercial carbonate electrolytes.Thence,developing stable SEI and dendrite-free LMA with the incorporation of LiNO_(3) into carbonate electrolytes is an efficacious strategy to realize robust LMBs via a scalable and cost-effective route.Therefore,this review unravels the grievances between LMA,LiNO_(3)and carbonate electrolytes,and enables a comprehensive analysis of LMA stabilizing mechanism with LiNO_(3),dissolution principle of LiNO_(3) in carbonate electrolytes,and LiNO_(3) introduction strategies.This review converges attention on a point that the LiNO_(3)-introduction into commercial carbonate electrolytes is an imperious choice to realize practical LMBs with commercial 4 V layered cathode.
基金supported by the National Natural Science Foundation of China (U20A2017)Guangdong Basic and Applied Basic Research Foundation (2022A1515010134,2022A1515110598)+2 种基金Youth Innovation Promotion Association of Chinese Academy of Sciences (2017120)Shenzhen-Hong Kong Institute of Brain Science–Shenzhen Fundamental Research Institutions (NYKFKT2019009)Shenzhen Technological Research Center for Primate Translational Medicine (F-2021-Z99-504979)。
文摘Accurately recognizing facial expressions is essential for effective social interactions.Non-human primates(NHPs)are widely used in the study of the neural mechanisms underpinning facial expression processing,yet it remains unclear how well monkeys can recognize the facial expressions of other species such as humans.In this study,we systematically investigated how monkeys process the facial expressions of conspecifics and humans using eye-tracking technology and sophisticated behavioral tasks,namely the temporal discrimination task(TDT)and face scan task(FST).We found that monkeys showed prolonged subjective time perception in response to Negative facial expressions in monkeys while showing longer reaction time to Negative facial expressions in humans.Monkey faces also reliably induced divergent pupil contraction in response to different expressions,while human faces and scrambled monkey faces did not.Furthermore,viewing patterns in the FST indicated that monkeys only showed bias toward emotional expressions upon observing monkey faces.Finally,masking the eye region marginally decreased the viewing duration for monkey faces but not for human faces.By probing facial expression processing in monkeys,our study demonstrates that monkeys are more sensitive to the facial expressions of conspecifics than those of humans,thus shedding new light on inter-species communication through facial expressions between NHPs and humans.
基金Supported by National Natural Science Foundation of China(No.81300805)。
文摘AIM:To characterize spectral-domain optical coherence tomography(SD-OCT)features of chorioretinal folds in orbital mass imaged using enhanced depth imaging(EDI).METHODS:Prospective observational case-control study was conducted in 20 eyes of 20 patients,the uninvolved eye served as a control.All the patients underwent clinical fundus photography,computed tomography,EDI SDOCT imaging before and after surgery.Two patients with cavernous hemangiomas underwent intratumoral injection of bleomycin A5;the remaining patients underwent tumor excision.Patients were followed 1 to 14mo following surgery(average follow up,5.8mo).RESULTS:Visual acuity prior to surgery ranged from 20/20 to 20/200.Following surgery,5 patients’visual acuity remained unchanged while the remaining 15 patients had a mean letter improvement of 10(range 4 to 26 letters).Photoreceptor inner/outer segment defects were found in 10 of 15 patients prior to surgery.Following surgical excision,photoreceptor inner/outer segment defects fully resolved in 8 of these 10 patients.CONCLUSION:Persistence of photoreceptor inner/outer segment defects caused by compression of the globe by an orbital mass can be associated with reduced visual prognosis.Our findings suggest that photoreceptor inner/outer segment defects on EDI SD-OCT could be an indicator for immediate surgical excision of an orbital mass causing choroidal compression.
基金Supported by the National Natural Science Foundation of China (No.82171099,No.82000940,No.81970835,No.81800867)the Natural Science Foundation of Shanghai (No.20ZR1409500)。
文摘AIM:To illustrate clinicopathological features of orbital non-rhabdomyosarcoma soft tissue sarcoma(NRSTS),and to compare the treatment outcome between postoperative radiotherapy(RT) and chemotherapy in a retrospective analysis nearly 20y.METHODS:A retrospective cohort study of 56 patients with orbital NRSTS were reviewed,34 of whom received postoperative RT,and 22 received postoperative chemotherapy.The clinicopathological features,local recurrence,metastases,and survival data were recorded.Survival analysis was performed using the Kaplan-Meier method.RESULTS:During follow-up(111.8mo,ranged 8-233mo) for 56 patients,19 patients of them developed local recurrence,and 7 patients developed distant metastases.Fifteen patients died during follow-up period.Overall survival rates considering the whole study group was 78.57% at 5y,and 72.16% at 10y after the initial diagnosis.Compared with chemotherapy,RT was associated with lower risk of local recurrence [hazard ratio for RT vs chemotherapy,0.263,95% confidence interval(CI),0.095-0.728,P=0.0015];with lower risk of distant metastasis(hazard ratio for RT vs chemotherapy,0.073,95%CI,0.015-0.364,P=0.0014);and with lower risk of death from disease(hazard ratio for RT vs chemotherapy,0.066,95%CI,0.022-0.200,P<0.0001).The 5-year survival rate in RT group was 97.06% compared to 50% in chemotherapy group.CONCLUSION:In patients with orbital NRSTS,postoperative RT provides better control of local recurrence,distant metastasis,and death from disease than chemotherapy.RT is the more preferrable adjuvant therapy compared to chemotherapy possibly.
文摘通过分析临床医学本科生耳鼻喉头颈外科学习中存在的教学困境,对教学质量满意度的影响因素进行分析,提出了一套创新的病例讨论教学法(case based study,CBS)结合传统教学方法(lecture based learning,LBL),即以病例讨论和理论知识为中心的临床教学法能充分发挥本科生的主现能动性,是提高本科生理论知识和实践操作等综合素质的有效途径。在传统授课中加入病例讨论教学,能使学生更好地掌握耳鼻咽喉头颈外科学的知识。在传统教学模式上的创新,可改善传统教学模式中枯燥、平述式教学模式,创新教学模式下有助于整体耳鼻喉科临床教学水平的提高。
基金the support from the Natural Science Foundation of China(No.51871118)Open Project of Key Laboratory for Magnetism and Magnetic Materials of the Ministry of Education,Lanzhou University,China(No.LZUMMM2021005)+1 种基金the Science and Technology Project of Lanzhou City,China(No.2019-1-30)State Key Laboratory of Special Rare Metal Materials,China(No.SKL2020K003)。
基金supported by the Combined pecific Foundation of Department of Science and Technology of Yunnan Province and Kunming Medical University,No.2008CD037
文摘The motor relearning program can significantly improve various functional disturbance induced by ischemic cerebrovascular diseases. However, its mechanism of action remains poorly understood. In injured brain tissues, glial fibrillary acidic protein and neurofilament protein changes can reflect the condition of injured neurons and astrocytes, while vascular endothelial growth factor and basic fibroblast growth factor changes can indicate angiogenesis. In the present study, we induced ischemic brain injury in the rhesus macaque by electrocoagulation of the M1 segment of the right middle cerebral artery. The motor relearning program was conducted for 60 days from the third day after model establishment. Immunohistochemistry and single-photon emission CT showed that the numbers of glial fibrillary acidic protein-, neurofilament protein-, vascular endothelial growth factor- and basic fibroblast growth factor-positive cells were significantly increased in the infarcted side compared with the contralateral hemisphere following the motor relearning program. Moreover, cerebral blood flow in the infarcted side was significantly improved. The clinical rating scale for stroke was used to assess neurological function changes in the rhesus macaque following the motor relearning program. Results showed that motor function was improved, and problems with consciousness, self-care ability and balance function were significantly ameliorated. These findings indicate that the motor relearning program significantly promoted neuronal regeneration, repair and angiogenesis in the surroundings of the infarcted hemisphere, and improve neurological function in the rhesus macaque following brain ischemia.