Grassland degradation can alter the structure and function of ecosystem and soil seed bank.Therefore,estimating the role of soil seed bank in vegetation regeneration of degraded grasslands is crucial.We selected grass...Grassland degradation can alter the structure and function of ecosystem and soil seed bank.Therefore,estimating the role of soil seed bank in vegetation regeneration of degraded grasslands is crucial.We selected grasslands with three levels of degradation,namely non-degraded(ND),mildly degraded(MD),and heavily degraded(HD)to analyze the effect of grassland degradation on soil seed bank,as well as the role of soil seed bank on vegetation regeneration of the alpine grasslands,China.Soil samples from each level were collected in May,before seedling emergence,in August,after completion of transient seed bank germination,and in December,after seed dispersal,to determine the seed density and species composition through germination experiment.Result showed that a total of 35 plant species was identified,including 15 species observed in both soil seed bank and above-ground vegetation.A total of 19,15,and 14 species of soil seed bank were identified in December,May,and August,respectively.The most abundant species in soil seed bank were Compositae(5 species),followed by Poaceae(4 species),and Cyperaceae(3 species).Degradation level has no significant impact on species richness and Shannon-Wiener index of soil seed bank.In addition,sampling month and grassland degradation affected soil seed bank density,in which December>May>August,and ND>MD>HD,indicating that density of transient seed bank was greater than persistent seed bank.Soil seed bank density of surface layer(0–5 cm)accounting for 42%–72%of the total density,which was significantly higher than that of deep layer(5–10 cm).Similarity of species composition between vegetation and soil seed bank was low,and it increased with degradation level(ranged from 0.14 to 0.69).We concluded that grassland degradation affects soil seed bank density more than species diversity,and soil seed bank contributed slightly to vegetation regeneration of degraded alpine grassland.Therefore,it is unlikely that degraded alpine meadow can be restored solely through soil seed bank.展开更多
目的:利用网络药理学技术和分子对接研究健脾益肾方治疗慢性肾脏病的作用机制。方法:通过中药系统药理学数据库和分析平台(TCMSP)及中医药整合药理学研究平台(Integrative Pharmacology-based Research Platform of Traditional Chinese...目的:利用网络药理学技术和分子对接研究健脾益肾方治疗慢性肾脏病的作用机制。方法:通过中药系统药理学数据库和分析平台(TCMSP)及中医药整合药理学研究平台(Integrative Pharmacology-based Research Platform of Traditional Chinese Medicine,TCMIP)v2.0收集健脾益肾方中八味中药的主要活性成分,通过TCMSP及Swiss Target Prediction数据库查询活性成分的靶点信息;从人类基因数据库(GeneCards)、《人类孟德尔遗传》数据库(OMIM)、Drugbank、疗效药靶数据库(TTD)中获取慢性肾脏病的相关靶标;取活性成分靶标与疾病靶标的交集,根据拓扑特征值筛选健脾益肾方治疗慢性肾脏病的关键靶标;将关键靶标进行GO功能分析和KEGG信号通路分析,并采用Cytoscape 3.7.0构建“中药-关键活性成分-核心靶标-信号通路”,并筛选其核心网络。结果:健脾益肾方中槲皮素、山柰酚、木犀草素等为其发挥作用的核心活性成分,雌激素受体1(ESR1)、雄激素受体(AR)、过氧化物酶体增殖物激活受体γ(PPARG)、前列腺素-内过氧化物合酶1(PTGS1)、核受体共激活剂2(NCOA2)等为其关键靶标,可能通过调控对药物的反应、凋亡过程的负调控、老化等生物学过程,作用于胞质溶胶、质膜、核质等细胞组分,影响酶结合、碳酸盐脱水酶活性、药物结合等分子功能,通过改善组织局部血供,促进血管生成,改善氧化应激状态,调节慢性炎症,改善甲状腺激素水平,改善慢性肾脏病的胰岛素抵抗,抑制慢性肾脏病细胞凋亡等信号通路发挥治疗慢性肾脏病的作用。分子对接结果显示,核心活性成分与核心靶标之间结合作用良好。结论:该研究基于网络药理学和分子对接的方法筛选健脾益肾方治疗慢性肾脏病的核心活性成分及其调控的关键靶标、信号通路,揭示了健脾益肾方通过多成分、多靶点、多通路改善慢性肾脏病的作用,为后续研究提供了丰富的信息和理论依据。展开更多
基金supported by the National Natural Science Foundation of China (31960279)the Science and Technology Program of Gansu Province, China (21JR11RA018)+1 种基金the Special Project for Central Universities to Build World-class Universities/Disciplines, and Characteristic Development GuidanceProgram for Changjiang Scholars and Innovative Research Team in University of Ministry of Education, China (IRT_17R88)
文摘Grassland degradation can alter the structure and function of ecosystem and soil seed bank.Therefore,estimating the role of soil seed bank in vegetation regeneration of degraded grasslands is crucial.We selected grasslands with three levels of degradation,namely non-degraded(ND),mildly degraded(MD),and heavily degraded(HD)to analyze the effect of grassland degradation on soil seed bank,as well as the role of soil seed bank on vegetation regeneration of the alpine grasslands,China.Soil samples from each level were collected in May,before seedling emergence,in August,after completion of transient seed bank germination,and in December,after seed dispersal,to determine the seed density and species composition through germination experiment.Result showed that a total of 35 plant species was identified,including 15 species observed in both soil seed bank and above-ground vegetation.A total of 19,15,and 14 species of soil seed bank were identified in December,May,and August,respectively.The most abundant species in soil seed bank were Compositae(5 species),followed by Poaceae(4 species),and Cyperaceae(3 species).Degradation level has no significant impact on species richness and Shannon-Wiener index of soil seed bank.In addition,sampling month and grassland degradation affected soil seed bank density,in which December>May>August,and ND>MD>HD,indicating that density of transient seed bank was greater than persistent seed bank.Soil seed bank density of surface layer(0–5 cm)accounting for 42%–72%of the total density,which was significantly higher than that of deep layer(5–10 cm).Similarity of species composition between vegetation and soil seed bank was low,and it increased with degradation level(ranged from 0.14 to 0.69).We concluded that grassland degradation affects soil seed bank density more than species diversity,and soil seed bank contributed slightly to vegetation regeneration of degraded alpine grassland.Therefore,it is unlikely that degraded alpine meadow can be restored solely through soil seed bank.
文摘目的:利用网络药理学技术和分子对接研究健脾益肾方治疗慢性肾脏病的作用机制。方法:通过中药系统药理学数据库和分析平台(TCMSP)及中医药整合药理学研究平台(Integrative Pharmacology-based Research Platform of Traditional Chinese Medicine,TCMIP)v2.0收集健脾益肾方中八味中药的主要活性成分,通过TCMSP及Swiss Target Prediction数据库查询活性成分的靶点信息;从人类基因数据库(GeneCards)、《人类孟德尔遗传》数据库(OMIM)、Drugbank、疗效药靶数据库(TTD)中获取慢性肾脏病的相关靶标;取活性成分靶标与疾病靶标的交集,根据拓扑特征值筛选健脾益肾方治疗慢性肾脏病的关键靶标;将关键靶标进行GO功能分析和KEGG信号通路分析,并采用Cytoscape 3.7.0构建“中药-关键活性成分-核心靶标-信号通路”,并筛选其核心网络。结果:健脾益肾方中槲皮素、山柰酚、木犀草素等为其发挥作用的核心活性成分,雌激素受体1(ESR1)、雄激素受体(AR)、过氧化物酶体增殖物激活受体γ(PPARG)、前列腺素-内过氧化物合酶1(PTGS1)、核受体共激活剂2(NCOA2)等为其关键靶标,可能通过调控对药物的反应、凋亡过程的负调控、老化等生物学过程,作用于胞质溶胶、质膜、核质等细胞组分,影响酶结合、碳酸盐脱水酶活性、药物结合等分子功能,通过改善组织局部血供,促进血管生成,改善氧化应激状态,调节慢性炎症,改善甲状腺激素水平,改善慢性肾脏病的胰岛素抵抗,抑制慢性肾脏病细胞凋亡等信号通路发挥治疗慢性肾脏病的作用。分子对接结果显示,核心活性成分与核心靶标之间结合作用良好。结论:该研究基于网络药理学和分子对接的方法筛选健脾益肾方治疗慢性肾脏病的核心活性成分及其调控的关键靶标、信号通路,揭示了健脾益肾方通过多成分、多靶点、多通路改善慢性肾脏病的作用,为后续研究提供了丰富的信息和理论依据。
