The essential and toxic element concentrations in buckwheat were analyzed by inductively coupled plasma optical emission spectrometer（ICP-OES）. The concentration data were subjected to common chemometrics analyses, ...The essential and toxic element concentrations in buckwheat were analyzed by inductively coupled plasma optical emission spectrometer（ICP-OES）. The concentration data were subjected to common chemometrics analyses, including correlation analysis（CA）, principal component analysis（PCA） and hierarchical cluster analysis（HCA）, to gain better understanding of the differences among the tested samples. Our results indicated that the essential and toxic element concentrations were not different between Fagopyrum tataricum（L.） Gaertn and F. esculentum Moench. The element concentrations varied among buckwheat samples from different sources. Commercial tartary buckwheat tea contained several essential elements, thus, could be used as the source of essential elements. The detection of toxic heavy metals in commercial tartary buckwheat tea suggested that safety issue of such buckwheat products should be seriously concerned. Our results also revealed that the place of origin and the processing protocol of tartary buckwheat affected the element concentrations of the commercial form. The implications to the quality control and safety evaluation of buckwheat were extensively discussed.展开更多
AIM: To study the hepatoprotective effect of methanol extract of Gentiana veitchiorum(MGV) against CCl4-induced oxidative stress and liver injury in mice. METHOD: The acute hepatic model was developed by injection of ...AIM: To study the hepatoprotective effect of methanol extract of Gentiana veitchiorum(MGV) against CCl4-induced oxidative stress and liver injury in mice. METHOD: The acute hepatic model was developed by injection of 20% CCl4 in mice. ICR mice were divided into six groups, including control, CCl4, CCl4+ silymarin, and CCl4+ MGV(100, 200, and 400 mg·kg–1) groups. Hepatic enzymes including AST, ALT and ALP levels in serum, and antioxidant enzymes, including SOD, CAT and GPX activity in liver tissue, were determined. Histopathological examination and Western blot analysis were performed. RESULTS: Oral administration of MGV at 200 and 400 mg·kg–1 for 15 days dose-dependently inhibited the serum elevations of AST, ALT, and ALP, and recovered the reduction of SOD, CAT, and GPX in liver tissue. Hematoxylin and eosin staining examination performed in liver tissues suggested that MGV treatment ameliorated histopathological changes in CCl4-induced mice. Western blotting analysis implied that MGV increased HO-1 expression and recovered TNF-α alternation. CONCLUSION: G. veitchiorum can protect the liver against CCl4-induced damage in mice, and this hepatoprotective effect was due at least in part to its ability through scavenging CCl4-associated free radical activities. The study provided in vivo evidence that G. veitchiorum can be used as a safe, cheap, and effective agent to reduce acute liver damage, supporting its folk medicine use.展开更多
Abstract: Objective To establish a new procedure for isolating scutellarin from Erigeron multiradiatus. Methods Aproposed method was developed by combining macroporous resins with ODS column. Firstly, E. multiradiatus...Abstract: Objective To establish a new procedure for isolating scutellarin from Erigeron multiradiatus. Methods Aproposed method was developed by combining macroporous resins with ODS column. Firstly, E. multiradiatus wasextracted by ultrasound with 80% methanol. Preliminary separation was performed on macroporous resin column.The performance and adsorption characteristics of three macroporous resins, D140, D141, and D605, were comparedand the enrichment procedure was optimized. Further purification was carried out by medium pressure liquidchromatography (MPLC) with ODS column. Results It was demonstrated that D141 had better extractive effects onscutellarin. The MPLC conditions were optimized as follows: 15% ethanol aqueous as mobile phase with flow rate at2.5 mL/min. The yield and purity of the isolated scutellarin were 1.20 mg/g and 96.5%, respectively. ConclusionThe overall procedure is efficient and low-cost, which is considered suitable for the separation and purification ofscutellarin from E. multiradiatus. The results provide the scientific basis for developing and using scutellarin in clinic.展开更多
基金the Key Technologies R&D Program of China during the 12th Five-Year Plan period(2012BAI27B07)the National Natural Science Foundation of China (81173653)+2 种基金the Administration of Traditional Chinese Medicine Department of Sichuan Province, China (2010-78)the Research Funds of Southwest University for Nationalities, China (13NLY01)the Earmarked Fund for China Agriculture Research System (CARS-08-D-3)
文摘The essential and toxic element concentrations in buckwheat were analyzed by inductively coupled plasma optical emission spectrometer（ICP-OES）. The concentration data were subjected to common chemometrics analyses, including correlation analysis（CA）, principal component analysis（PCA） and hierarchical cluster analysis（HCA）, to gain better understanding of the differences among the tested samples. Our results indicated that the essential and toxic element concentrations were not different between Fagopyrum tataricum（L.） Gaertn and F. esculentum Moench. The element concentrations varied among buckwheat samples from different sources. Commercial tartary buckwheat tea contained several essential elements, thus, could be used as the source of essential elements. The detection of toxic heavy metals in commercial tartary buckwheat tea suggested that safety issue of such buckwheat products should be seriously concerned. Our results also revealed that the place of origin and the processing protocol of tartary buckwheat affected the element concentrations of the commercial form. The implications to the quality control and safety evaluation of buckwheat were extensively discussed.
基金supported by the National KeyTechnology R&D Program in the 12th Five year Plan of China(No.2012BAI27B07)the Macao Science and Technology Development Fund(No.073/2011/A3)
文摘AIM: To study the hepatoprotective effect of methanol extract of Gentiana veitchiorum(MGV) against CCl4-induced oxidative stress and liver injury in mice. METHOD: The acute hepatic model was developed by injection of 20% CCl4 in mice. ICR mice were divided into six groups, including control, CCl4, CCl4+ silymarin, and CCl4+ MGV(100, 200, and 400 mg·kg–1) groups. Hepatic enzymes including AST, ALT and ALP levels in serum, and antioxidant enzymes, including SOD, CAT and GPX activity in liver tissue, were determined. Histopathological examination and Western blot analysis were performed. RESULTS: Oral administration of MGV at 200 and 400 mg·kg–1 for 15 days dose-dependently inhibited the serum elevations of AST, ALT, and ALP, and recovered the reduction of SOD, CAT, and GPX in liver tissue. Hematoxylin and eosin staining examination performed in liver tissues suggested that MGV treatment ameliorated histopathological changes in CCl4-induced mice. Western blotting analysis implied that MGV increased HO-1 expression and recovered TNF-α alternation. CONCLUSION: G. veitchiorum can protect the liver against CCl4-induced damage in mice, and this hepatoprotective effect was due at least in part to its ability through scavenging CCl4-associated free radical activities. The study provided in vivo evidence that G. veitchiorum can be used as a safe, cheap, and effective agent to reduce acute liver damage, supporting its folk medicine use.
基金Fundamental Research Funds for Central Universities,Southwest University for Nationalities(09NZYZJ01)National Key Technology R&D Program in the 12th Five Year Plan of China(2012BAI27B07)
文摘Abstract: Objective To establish a new procedure for isolating scutellarin from Erigeron multiradiatus. Methods Aproposed method was developed by combining macroporous resins with ODS column. Firstly, E. multiradiatus wasextracted by ultrasound with 80% methanol. Preliminary separation was performed on macroporous resin column.The performance and adsorption characteristics of three macroporous resins, D140, D141, and D605, were comparedand the enrichment procedure was optimized. Further purification was carried out by medium pressure liquidchromatography (MPLC) with ODS column. Results It was demonstrated that D141 had better extractive effects onscutellarin. The MPLC conditions were optimized as follows: 15% ethanol aqueous as mobile phase with flow rate at2.5 mL/min. The yield and purity of the isolated scutellarin were 1.20 mg/g and 96.5%, respectively. ConclusionThe overall procedure is efficient and low-cost, which is considered suitable for the separation and purification ofscutellarin from E. multiradiatus. The results provide the scientific basis for developing and using scutellarin in clinic.
