Expert systems for HPLC in biochemistry—The knowledge base for selection of HPLC column system in the separation of amino acids, peptides and proteins

The strategy of expert system for high performance liquid chromatography was discussed, the attentions are mainly placed on the knowledge base for selection of column system, separation modes and detection modes in the analysis of amino acids, peptides and proteins.

The principle of optimization of binary mobile phase composition of multi-step linear gradient elution

The basic principle of optimal method called “moving overlapping resolution mapping Method” to select the optimal binary mobile phase composition of multi-step linear gradient liquid chromatography is discussed with simultaneously considering effects of position of solute inside the column and mobile phase composition on peak resolution and retention value, then a BASIC program based on this principle is developed in IBM-PC computer. The validities of both principle of optimization and BASIC program are confirmed by separation of samples Containing bile acids and PAHs in RP-HPLC.