为了明确马铃薯杂交组合‘MH-09’בMIN-21’F_(1)代分离群体中选育出的8个优良杂种株系间的分子细胞遗传学差异,为后续新品种育成及鉴定提供依据,利用亲本材料作对照,对其花粉育性、PMCMⅠ染色体配对行为及SRAP(Sequence related...为了明确马铃薯杂交组合‘MH-09’בMIN-21’F_(1)代分离群体中选育出的8个优良杂种株系间的分子细胞遗传学差异,为后续新品种育成及鉴定提供依据,利用亲本材料作对照,对其花粉育性、PMCMⅠ染色体配对行为及SRAP(Sequence related amplified polymorphism,序列相关扩增多态性)指纹特征等进行了比较研究.结果表明:各杂种株系间的花粉可育率存在一定差异,变幅为48.1%~77.3%,株系ZX-1540的花粉育性低于50%,其余株系均在55%以上;8个杂种株系ZX-20、ZX-460、ZX-780、ZX-900、ZX-1120、ZX-1540、ZX-1960和ZX-2200的PMCMⅠ染色体配对构型分别是3.82Ⅰ+13.05Ⅱ+2.31Ⅲ+2.79Ⅳ,6.52Ⅰ+13.04Ⅱ+2.24Ⅲ+2.17Ⅳ,4.37Ⅰ+9.97Ⅱ+3.19Ⅲ+3.53Ⅳ,4.05Ⅰ+15.54Ⅱ+2.12Ⅲ+1.63Ⅳ,2.71Ⅰ+13.85Ⅱ+2.35Ⅲ+2.64Ⅳ,8.44Ⅰ+7.08Ⅱ+5.92Ⅲ+1.91Ⅳ,5.20Ⅰ+8.61Ⅱ+6.76Ⅲ+1.33Ⅳ和1.13Ⅰ+11.69Ⅱ+1.04Ⅲ+5.09Ⅳ;利用筛选出的10对SRAP适宜引物,对10个材料进行PCR扩增得到122个多态性位点条带,多态性比率为65.92%;用特异性引物M5BE7扩增建立了能鉴定8个优良杂种株系和其亲本的SRAP指纹图.各株系及其亲本的遗传距离(GD)范围在0.290~0.640之间.以GD值0.40作基准,将10个材料划分成5类:株系ZX-20、ZX-1960和母本‘MH-09’为一类;株系ZX-780、ZX-1120、ZX-2200和父本‘MIN-21’为一类;株系ZX-460、ZX-900和ZX-1540各为一类.展开更多
为了解决新疆某铀矿床采区钻井过滤器损坏、钻井无法继续使用的问题,尝试使用内置过滤器技术。以该矿床4#采区为例,分析了前期修复过程中切割、内置过滤器技术存在的问题,并在该采区再次进行了过滤器修复试验。结果表明:通过采用二次钻...为了解决新疆某铀矿床采区钻井过滤器损坏、钻井无法继续使用的问题,尝试使用内置过滤器技术。以该矿床4#采区为例,分析了前期修复过程中切割、内置过滤器技术存在的问题,并在该采区再次进行了过滤器修复试验。结果表明:通过采用二次钻进、优化切割刀具结构、设计制作内置过滤器铆固装置等方法可以解决修复过程存在的问题;钻井修复后,浸出效果得到提升,4-0206#试验单元抽液量由1.5 m 3/h上升至2.9 m 3/h,浸出液铀质量浓度由15.8 mg/L上升至35.3 mg/L,取得了良好的效果。展开更多
The relationship between the electronic structures of manganese nitrido complexes and the substituted ligands is investigated by using density functional theory.By designing a series of manganese nitrido complexes [Mn...The relationship between the electronic structures of manganese nitrido complexes and the substituted ligands is investigated by using density functional theory.By designing a series of manganese nitrido complexes [Mn（SalenR）N]＋ with different para ring substituents（R = H,CH_3,NH_2,OCH_3,NMeF,etc） of the ancillary ligand,the properties of manganese-nitrogen bonds were compared for two kinds of electronic structures,of which the radical resides on metal center or the coordinated ring ligand.Our calculation shows that for R = H,CH_3 and NH_2,the [Mn（SalenR）N]＋ complexes have a high-valent Mn（VI） center,and for R = OCH_3 and NMeF,the complexes represent a configuration where the radical delocalizes on the ligand.It is found that the relative energies of these two species depend on electronic properties of the substituent,originating from the intrinsic property of HOMO-LUMO gaps.展开更多
文摘为了明确马铃薯杂交组合‘MH-09’בMIN-21’F_(1)代分离群体中选育出的8个优良杂种株系间的分子细胞遗传学差异,为后续新品种育成及鉴定提供依据,利用亲本材料作对照,对其花粉育性、PMCMⅠ染色体配对行为及SRAP(Sequence related amplified polymorphism,序列相关扩增多态性)指纹特征等进行了比较研究.结果表明:各杂种株系间的花粉可育率存在一定差异,变幅为48.1%~77.3%,株系ZX-1540的花粉育性低于50%,其余株系均在55%以上;8个杂种株系ZX-20、ZX-460、ZX-780、ZX-900、ZX-1120、ZX-1540、ZX-1960和ZX-2200的PMCMⅠ染色体配对构型分别是3.82Ⅰ+13.05Ⅱ+2.31Ⅲ+2.79Ⅳ,6.52Ⅰ+13.04Ⅱ+2.24Ⅲ+2.17Ⅳ,4.37Ⅰ+9.97Ⅱ+3.19Ⅲ+3.53Ⅳ,4.05Ⅰ+15.54Ⅱ+2.12Ⅲ+1.63Ⅳ,2.71Ⅰ+13.85Ⅱ+2.35Ⅲ+2.64Ⅳ,8.44Ⅰ+7.08Ⅱ+5.92Ⅲ+1.91Ⅳ,5.20Ⅰ+8.61Ⅱ+6.76Ⅲ+1.33Ⅳ和1.13Ⅰ+11.69Ⅱ+1.04Ⅲ+5.09Ⅳ;利用筛选出的10对SRAP适宜引物,对10个材料进行PCR扩增得到122个多态性位点条带,多态性比率为65.92%;用特异性引物M5BE7扩增建立了能鉴定8个优良杂种株系和其亲本的SRAP指纹图.各株系及其亲本的遗传距离(GD)范围在0.290~0.640之间.以GD值0.40作基准,将10个材料划分成5类:株系ZX-20、ZX-1960和母本‘MH-09’为一类;株系ZX-780、ZX-1120、ZX-2200和父本‘MIN-21’为一类;株系ZX-460、ZX-900和ZX-1540各为一类.
文摘为了解决新疆某铀矿床采区钻井过滤器损坏、钻井无法继续使用的问题,尝试使用内置过滤器技术。以该矿床4#采区为例,分析了前期修复过程中切割、内置过滤器技术存在的问题,并在该采区再次进行了过滤器修复试验。结果表明:通过采用二次钻进、优化切割刀具结构、设计制作内置过滤器铆固装置等方法可以解决修复过程存在的问题;钻井修复后,浸出效果得到提升,4-0206#试验单元抽液量由1.5 m 3/h上升至2.9 m 3/h,浸出液铀质量浓度由15.8 mg/L上升至35.3 mg/L,取得了良好的效果。
基金supported by the Project of National Natural Science Foundation of China(No.21573237,21603227,21403242,21703246)Natural Science Foundation of Fujian Province(2017J05032)
文摘The relationship between the electronic structures of manganese nitrido complexes and the substituted ligands is investigated by using density functional theory.By designing a series of manganese nitrido complexes [Mn（SalenR）N]＋ with different para ring substituents（R = H,CH_3,NH_2,OCH_3,NMeF,etc） of the ancillary ligand,the properties of manganese-nitrogen bonds were compared for two kinds of electronic structures,of which the radical resides on metal center or the coordinated ring ligand.Our calculation shows that for R = H,CH_3 and NH_2,the [Mn（SalenR）N]＋ complexes have a high-valent Mn（VI） center,and for R = OCH_3 and NMeF,the complexes represent a configuration where the radical delocalizes on the ligand.It is found that the relative energies of these two species depend on electronic properties of the substituent,originating from the intrinsic property of HOMO-LUMO gaps.
