期刊文献+
共找到1篇文章
< 1 >
每页显示 20 50 100
B to O erythrocyte conversion by the recombinant α-galactosidase 被引量:13
1
作者 ZHANG Yang-pei GONG Feng +14 位作者 BAO Guo-qiang GAO Hong-wei JI Shou-ping TAN Ying-xia LI Su-bo LI Li-li WANG Ying-li XU Hua XU Li-juan TIAN Shu-guang ZHANG Zhi-xin lu qiu-shuang QIU Yan BAI Jian-shi CHEN Ji-ting 《Chinese Medical Journal》 SCIE CAS CSCD 2007年第13期1145-1150,共6页
Background Human group O red blood cells have great benefit in specialized transfusion areas such as armed conflict and natural calamity. The group B antigen differs structurally from group O antigen only by the addit... Background Human group O red blood cells have great benefit in specialized transfusion areas such as armed conflict and natural calamity. The group B antigen differs structurally from group O antigen only by the addition of one terminal α-linked galactose residue. In this study we aimed to remove the terminal galactose from group B red blood cell to get group O red blood cell. Methods α-galactosidase cDNA was cloned by RT-PCR from Catimor coffee beans grown on Hainan Island of China. The vector for α-galactosidase cDNA expression was constructed and transferred into Pichia pastoris cells by electroporation. The transgenic cells were cloned by fermentation and the recombinant α-galactosidase was purified by ion exchange chromatography. After studying the biochemical characters of α-galactosidase, we have used it in converting human erythrocytes from group B to group O. Results The purity of recombinant α-galactosidase was higher than 96%, which was thought to be suitable for the use of blood conversion. Enzymatically converted human group O red blood cells (ECHORBC) exhibited membrane integrity, metabolic integrity, normal cell deformation and morphology. There were no coagulation between ECHORBC and any group of human blood. The ECHORBC will keep normal structure and function for a period of 21 days at 4℃ in monoammoniumphosphate nutrient solution. Experiments with Rhesus monkeys and gibbons showed that transfusion of enzymatically converted erythrocytes was safe. Conclusion ECHORBC can be easily obtained from group B red blood cell by α-galactosidase digestion. This study suggests that ECHORBC could be transfused to patients safely and efficiently. 展开更多
关键词 erythrocytes blood conversion Α-GALACTOSIDASE
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部