地佐辛超前镇痛对隐匿性阴茎矫治术患儿苏醒期谵妄的临床观察

【目的】观察地佐辛超前镇痛对隐匿性阴茎矫治术患儿苏醒期谵妄(ED)的影响。【方法】筛选2020年7月至2020年10月62例6~10岁拟行隐匿性阴茎矫治术的男孩,按随机数字表法分为生理盐水对照组(C组,n=32例)和地佐辛组(D组,n=30例),所有患者均采用静吸复合全麻(喉罩)。2组分别在麻醉诱导前5 min给予等容量的0.9%生理盐水10 mL或地佐辛0.1 mg/kg(0.9%生理盐水稀释至10 mL)。比较2组入PACU即刻(T1)、10 min(T2)、出PACU即刻(T3)、术后1 h(T4)、术后6 h(T5)PAED评分及FLACC疼痛评分;比较2组术后ED、导尿管相关膀胱不适(CRBD)发生率。问卷患儿父母术后48 h内新发适应不良行为发生情况并比较;比较2组麻醉诱导前30 min(T0)、T1、T4、T5外周静脉血皮质醇(Cor)、肾上腺素(E)、血糖(Glu)水平。【结果】D组在T2~T5点PAED评分明显低于C组(P<0.001);FLACC评分低于C组(P<0.001);D组ED的发生率明显低于C组(16.67%vs.40.63%,P=0.039),术后48 h内新发适应不良行为的总发生率低于C组(13.33%vs.37.50%,P=0.042);CRBD发生率低于C组(6.67%vs.28.13%,P=0.044)。D组在T1、T4、T5点E、Cor、Glu水平明显低于C组(组间P值分别为P<0.001,P=0.009,P=0.012)。【结论】地佐辛超前镇痛可明显降低小儿隐匿性阴茎术后ED的发生率;降低术后新发适应不良行为的发生率,可能与地佐辛降低术后疼痛应激及CRBD的发生率有关。

DoS攻击下网络化控制系统基于观测器的记忆型事件触发预测控制

本文研究了DoS攻击下网络化控制系统记忆型事件触发预测补偿控制问题.首先,由于网络带宽资源有限和系统状态不完全可观测性,引入了记忆型事件触发函数,为观测器提供离散事件触发传输方案.然后,分析了网络传输通道上发生的DoS攻击.结合上述记忆型事件触发方案,在控制节点设计一类新颖的预测控制算法,节省网络带宽资源并主动补偿DoS攻击.同时,建立了基于观测器的记忆型事件触发预测控制的闭环系统,并且分析稳定性.通过线性矩阵不等式(LMI)和Lyapunov稳定性理论,建立了控制器、观测器和记忆型事件触发矩阵的联合设计方案,并验证了该方案的可行性.仿真结果表明,该方案结合记忆型事件触发机制可以有效补偿DoS攻击,节约网络带宽资源.

Bioaccumulation,subcellular distribution and chemical forms of cadmium in Aster subulatus Michx.

【Objective】Through analyzing the bioaccumulation capacity,subcellular distribution and chemical forms of cadmium(Cd)in Aster subulatus Michx.,this study was to provide reference for revealing the Cd tolerance mechanism of A.subulatus Michx.【Method】After cultured for 24 d under the action of Hoagland nutrient solution and gradient Cd concentrations(0,30,60 and 90 mg/L),A.subulatus Michx.were harvested,and its leaf,stem and root were treated by differential centrifugation,chemical reagent extraction,and digested with graphite digester,respectively,then the Cd content in the root,stem and leaf were determined by atomic absorption spectroscopy.【Result】The experimental results indicated that the bioaccumulation capacity of Cd in A.subulatus Michx.was root>stem>leaf,and the maximum Cd concentration in the root,stem and leaf of A.subulatus Michx.were 130.74,78.69 and 56.62 mg/kg(fresh matter),respectively.Most of Cd stored in the cell wall and the soluble fractions of the root and leaf of A.subulatus Michx.,with only a smaller portion Cd in organelle fraction.Analysis result of subcellular Cd content showed that 52.27%-58.61%of Cd for root was mainly stored in the soluble fraction,but 42.10%-63.28%of Cd for leaf was mainly stored in the cell wall fraction.The concentration of pectates and protein integrated-Cd was higher in the root and leaf compared to other chemical forms Cd.Pectates and protein integrated-Cd was the main chemical forms Cd in the root and leaf of A.subulatus Michx.,and their percentages were 68.91%-74.80%and 57.38%-83.80%,respectively.Cd treatment could significantly increase the proportion of water-soluble organic acid Cd from 13.64%to 22.72%in root and undissolved phosphate Cd from 10.02%to 32.78%in leaf with increasing Cd concentration in the culture medium.【Conclusion】The root,stem and leaf of A.subulatus Michx.has strong bioaccumulation capacity to Cd,Cd is primarily stored in the soluble fractions of the root and cell wall fractions of the leaf,and less toxic pectates and protein integrated-Cd is the main chemical forms Cd in the root and leaf of A.subulatus Michx.,this might be the main mechanism of Cd tolerance in A.subulatus Michx.