Reasonable nitrogen(N) application is a promising strategy for reducing crop cadmium(Cd) toxicity. However, the specific form of N and the required amount that affect Cd tolerance and accumulation in rice remain uncle...Reasonable nitrogen(N) application is a promising strategy for reducing crop cadmium(Cd) toxicity. However, the specific form of N and the required amount that affect Cd tolerance and accumulation in rice remain unclear. This study explored the influence of different N-fertilizer forms(NH_4NO_3, NH_4Cl, and KNO_3) and dosages on Cd tolerance and uptake in Cd-stressed N-sensitive and N-insensitive indica rice accessions. The results indicated that the Cd tolerance of N-sensitive indica accessions is more robust than that of N-insensitive ones. Furthermore, the shoot Cd content and Cd translocation rate in both N-sensitive and N-insensitive indica accessions decreased with an appropriate supply of NH_4NO_3 and NH_4Cl, whereas they were comparable or slightly increased with increased KNO_3. Unfortunately, we did not find significant and regular differences in Cd accumulation or translocation between N-sensitive and N-insensitive rice accessions. Consistent with the reduction of shoot Cd content, the addition of NH_4NO_3 and NH_4Cl also inhibited the instantaneous root Cd^(2+) uptake. The expression changes of Cd transport-related genes under different N forms and dosages suggested that the decreased shoot Cd content, caused by the increased supply of NH_4NO_3 and NH_4Cl, is likely achieved by reducing the transcription of OsNRAMP1 and OsIRT1. In summary, our findings reveal that an appropriate supply of NH_4NO_3 and NH_4Cl could reduce Cd uptake and transport in rice seedlings, suggesting that rational N management could reduce the Cd risk in rice production.展开更多
农业干旱监测问题对农业生产具有重要影响,因此精确监测农业干旱具有现实意义。该研究基于MOD16A2全球蒸散产品,计算作物缺水指数(Crop Water Stress Index,CWSI),结合地表温度、植被指数、降水量以及土壤湿度等多源遥感数据为自变量,以...农业干旱监测问题对农业生产具有重要影响,因此精确监测农业干旱具有现实意义。该研究基于MOD16A2全球蒸散产品,计算作物缺水指数(Crop Water Stress Index,CWSI),结合地表温度、植被指数、降水量以及土壤湿度等多源遥感数据为自变量,以3个月时间尺度的标准化降水蒸散指数(Standardized Precipitation Evapotranspiration Index,SPEI-3)为因变量,基于偏差校正随机森林算法构建山东省2000—2019年作物生长季(4—10月)的偏差校正随机森林干旱状况指数(Bias-corrected Random Forest Drought Condition Index,BRF-DCI)。并分析CWSI对于构建山东省农业干旱监测模型的影响。结果表明:加入CWSI后,所提出的BRF-DCI指数与SPEI-3观测指数的决定系数为0.72~0.85,优于未加入CWSI之前;加入CWSI后提高了干旱等级监测的准确率;BRF-DCI指数能较好地拟合各月份的SPEI-3指数,决定系数均在0.94以上;BRF-DCI指数能够准确反映山东省典型干旱年的干旱情况,有效监测山东省农业干旱情况。该研究对山东省农业旱情监测及旱灾防御具有较大的应用潜力。展开更多
基金supported by the National Natural Science Foundation of China (Grant No. 31971872)the Open Research Fund of State Key Laboratory of Hybrid Rice, China (Grant No. 2022KF02)+3 种基金the National Natural Science Foundation of China (Grant Nos. 32101755 and 32188102)the Zhejiang Provincial Natural Science Foundation, China (Grant No. LY22C130005)the Key Research and Development Program of Zhejiang Province, China (Grant No. 2021C02056)the ‘Pioneer’ and ‘Leading Goose’ R&D Program of Zhejiang, China (Grant No. 2023C02014)。
文摘Reasonable nitrogen(N) application is a promising strategy for reducing crop cadmium(Cd) toxicity. However, the specific form of N and the required amount that affect Cd tolerance and accumulation in rice remain unclear. This study explored the influence of different N-fertilizer forms(NH_4NO_3, NH_4Cl, and KNO_3) and dosages on Cd tolerance and uptake in Cd-stressed N-sensitive and N-insensitive indica rice accessions. The results indicated that the Cd tolerance of N-sensitive indica accessions is more robust than that of N-insensitive ones. Furthermore, the shoot Cd content and Cd translocation rate in both N-sensitive and N-insensitive indica accessions decreased with an appropriate supply of NH_4NO_3 and NH_4Cl, whereas they were comparable or slightly increased with increased KNO_3. Unfortunately, we did not find significant and regular differences in Cd accumulation or translocation between N-sensitive and N-insensitive rice accessions. Consistent with the reduction of shoot Cd content, the addition of NH_4NO_3 and NH_4Cl also inhibited the instantaneous root Cd^(2+) uptake. The expression changes of Cd transport-related genes under different N forms and dosages suggested that the decreased shoot Cd content, caused by the increased supply of NH_4NO_3 and NH_4Cl, is likely achieved by reducing the transcription of OsNRAMP1 and OsIRT1. In summary, our findings reveal that an appropriate supply of NH_4NO_3 and NH_4Cl could reduce Cd uptake and transport in rice seedlings, suggesting that rational N management could reduce the Cd risk in rice production.
