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Generation and Characterization of Monoclonal Antibodies Against Red-Spotted Grouper Nervous Necrosis Virus(RGNNV)
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作者 QIN Yinghui LIU Jiaoyun +1 位作者 lu yuanan LIU Xueqin 《Journal of Ocean University of China》 SCIE CAS CSCD 2022年第4期1061-1067,共7页
Nervous necrosis virus(NNV)can infect more than 120 fish species worldwide and has caused high mortality and sig-nificant economic losses to the aquaculture industry.Among different genotypes of NNV,the red-grouper ne... Nervous necrosis virus(NNV)can infect more than 120 fish species worldwide and has caused high mortality and sig-nificant economic losses to the aquaculture industry.Among different genotypes of NNV,the red-grouper nervous necrosis virus(RGNNV)is the most widely distributed one with the highest number of susceptible fish species.In this study,the capsid protein(Cp)gene of RGNNV was recombined and expressed in Escherichia coli strain BL21(DE3)and the recombinant Cp(rCp)was used as an immunogen to produce monoclonal antibodies(MAbs)through hybridoma cell fusion technology.Three MAbs were produced and characterized by indirect enzyme-linked immunosorbent assay(ELISA),western blotting,and immunofluorescence assay(IFA).Wes-tern blotting result showed that the MAbs could specifically react with the capsid protein of RGNNV.The result of IFA showed that the MAbs could recognize virions in RGNNV-infected grouper spleen(GS)cells.These results indicate that the MAbs can specifi-cally recognize RGNNV virions and can be used to produce a rapid detection method.This study provides a foundation for further studies on the rapid diagnosis of RGNNV and its infection mechanisms. 展开更多
关键词 RGNNV monoclonal antibody capsid protein GROUPER
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基于宏基因组测序技术研究南方地区健康与腹泻林麝肠道菌群特征
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作者 陆元安 李晓冰 +4 位作者 曾雅婷 王雅婷 林明生 陆林姬 吴琼 《动物营养学报》 CAS 2024年第11期7346-7357,共12页
本试验旨在探究腹泻对林麝肠道菌群组成和耐药基因的影响。选取36月龄健康林麝(SZ)和腹泻林麝(SF)各3头,采集所有个体粪便并进行宏基因组测序分析。结果表明:SF组Shannon指数降低,有益菌梭菌属(Clostridium)和Lactonifactor longovifor... 本试验旨在探究腹泻对林麝肠道菌群组成和耐药基因的影响。选取36月龄健康林麝(SZ)和腹泻林麝(SF)各3头,采集所有个体粪便并进行宏基因组测序分析。结果表明:SF组Shannon指数降低,有益菌梭菌属(Clostridium)和Lactonifactor longoviformis的丰度显著低于SZ组(P<0.05),而致病菌埃希氏菌属(Escherichia)、大肠杆菌(Escherichia coli)的丰度显著高于SZ组(P<0.05)。进一步分析肠道菌群功能,在CAZy功能分析中,差异显著基因来自糖苷水解酶类、糖基转移酶类、多糖裂解酶类和碳水化合物结合横块;在CARD抗性基因分析中,林麝主要抗性基因由9大类组成,占比较多的是利福霉素类(45.65%)、林可酰胺类(10.29%)和多肽类(7.11%);抗性基因来源较多的菌门主要有3个,分别为厚壁菌门(Firmicutes)、拟杆菌门(Bacteroidetes)和变形菌门(Proteobacteria);LEfSe分析显示,SF组显著抗性基因多于SZ组,主要由变形菌门贡献。综上所述,腹泻会导致林麝肠道菌群多样性降低,肠道致病菌和抗性基因增加,表明临床上对细菌性腹泻治疗要注重细菌耐药性。 展开更多
关键词 林麝 腹泻 宏基因组测序 肠道菌群 耐药基因
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