Objective:To explore the molecular basis of hepatocarcinogenesis by cloning and expressing a novel liver cancer apoptosis -related gene.Methods:With homologous screening and RT-PCR,we had cloned an apoptosis-related g...Objective:To explore the molecular basis of hepatocarcinogenesis by cloning and expressing a novel liver cancer apoptosis -related gene.Methods:With homologous screening and RT-PCR,we had cloned an apoptosis-related gene APG from liver cancer cells,compared its expression in hepatocellular carcinoma(HCC) tissue and paracarcinoma tissue,and analyzed its sequence from these tissues.The association of APG gene expression with HCC was investigated.Results:A new gene APG was cloned with a full-legth cDNA of 563 bp.Sequencing analysis showed heterogeneity of APG gene from hepatocarcinoma tissue and from paracarcinoma tissue.Among 50 cases of liver cancer,APG gene expressions were down-regulated in 42 cases（84%) ,while up-regulated in 8 cases(16%,P<0.01),Its expression was also found to be associated with tumor size(P<0.05),HbsAg level(P<0.01),degree of tumor differentiation(P<0.01),Ki-67 protein expression(P<0.05),p53 protein expression(P<0.01) and apoptosis(P<0.05),There is no association between the gene expression ,gender and AFP(P>0.05).Conclusion APG is an appoptosis-relate gene and down-regualted in HCC.Its expression is associated with many clinical and pathologic features of HCC,suggesting that APG gene is probably involved in the tumorigenesis of HCC.展开更多
文摘Objective:To explore the molecular basis of hepatocarcinogenesis by cloning and expressing a novel liver cancer apoptosis -related gene.Methods:With homologous screening and RT-PCR,we had cloned an apoptosis-related gene APG from liver cancer cells,compared its expression in hepatocellular carcinoma(HCC) tissue and paracarcinoma tissue,and analyzed its sequence from these tissues.The association of APG gene expression with HCC was investigated.Results:A new gene APG was cloned with a full-legth cDNA of 563 bp.Sequencing analysis showed heterogeneity of APG gene from hepatocarcinoma tissue and from paracarcinoma tissue.Among 50 cases of liver cancer,APG gene expressions were down-regulated in 42 cases（84%) ,while up-regulated in 8 cases(16%,P<0.01),Its expression was also found to be associated with tumor size(P<0.05),HbsAg level(P<0.01),degree of tumor differentiation(P<0.01),Ki-67 protein expression(P<0.05),p53 protein expression(P<0.01) and apoptosis(P<0.05),There is no association between the gene expression ,gender and AFP(P>0.05).Conclusion APG is an appoptosis-relate gene and down-regualted in HCC.Its expression is associated with many clinical and pathologic features of HCC,suggesting that APG gene is probably involved in the tumorigenesis of HCC.
