With a production of 208.2 m3/d, heavy oil was produced by drill stem test (DST) from three shallow reservoirs in Sand Group Nos. Ⅰ and Ⅲ of the Neogene Guantao Formation (NgⅠ and NgⅢ) and the Eogene Dongying Form...With a production of 208.2 m3/d, heavy oil was produced by drill stem test (DST) from three shallow reservoirs in Sand Group Nos. Ⅰ and Ⅲ of the Neogene Guantao Formation (NgⅠ and NgⅢ) and the Eogene Dongying Formation (Ed) in an exploratory well Ban-14-1 within the Qianmiqiao region, Bohai Bay Basin, northern China. Based on the GC and GC-MS data of the NgⅠ and NgⅢ heavy oil samples, all n-alkanes and most isoprenoid hydrocarbons are lost and the GC baseline appears as an evident 'hump', implying a large quantity of unresolved complex mixture (UCM), which typically revealed a result of heavy biodegradation. However, there still is a complete series of C14-C73 n-alkanes in the high-temperature gas chromatograms (HTGC) of the heavy oil, among which, the abundance of C30- n-alkanes are drastically reduced. The C35-C55 high molecular weight (HMW) n-alkanes are at high abundance and show a normal distribution pattern with major peak at C43 and an obvious odd-carbon-number predominance with CPI37-55 and OEP45-49 values of 1.17 and 1.16-1.20, respectively. According to GC-MS analysis, the heavy oil is characterized by dual source inputs of aquatic microbes and terrestrial higher plants. Various steranes and tricyclic terpanes indicate an algal origin, and hopane-type triterpanes, C24 tetracyclic terpane and drimane series show the bacterial contribution. With the odd-carbon-number preference, HMW n-alkanes provide significant information not only on higher plant source input and immaturity, but also on the strong resistibility to biodegradation.展开更多
A azoreductase gene with 537 bp was obtained by PCR amplification from Rhodobacter sphaeroides AS1 1737 The enzyme, with a molecular weight of 18 7 kD, was efficiently expressed in Escherichia coli and its biodegr...A azoreductase gene with 537 bp was obtained by PCR amplification from Rhodobacter sphaeroides AS1 1737 The enzyme, with a molecular weight of 18 7 kD, was efficiently expressed in Escherichia coli and its biodegradation characteristics for azo dyes were investigated. Furthermore, the reaction kinetics and mechanism of azo dyes catalyzed by the genetically engineered azoreductase were studied in detail. The presence of a hydrazo-intermediate was identified, which provided a convincing evidence for the assumption that azo dyes were degraded via an incomplete reduction stage.展开更多
Auto anti-collision technology is one of the main research subjects of automobiles’ safety technology. Aiming at the key technology of Auto anti-collision, measuring the distance from obstacles, based on the theory o...Auto anti-collision technology is one of the main research subjects of automobiles’ safety technology. Aiming at the key technology of Auto anti-collision, measuring the distance from obstacles, based on the theory of phase laser distance ranging, Laser Diode (LD) distance-measuring system for auto anti-collision has been developed to solve the problem of on-line measuring distance technology in middle to long distance utilizing the good characteristics of LD when modulating its optical intensity and adopting typical kinds of filter techniques in this paper. By theoretical analysis, adopting typical kinds of filter techniques can reduce the interference of strong light, so distance-measuring range can be 0.5–100 m in daytime or 1–200 m at night. And more, from theoretical analysis and experiment result, it can guarantee the high measuring resolution which can be less than 24.5 mm, utilizing the method of two Laser Diode optical intensity modulating wavelength and complimenting precise calibration and revision. The idea of LD distance-measuring technology is novel and feasible and this technology can be applied in Auto anti-collision. Key words laser diode - phase laser distance ranging - filter techniques - auto anti-collision CLC number TH 161 Foundation item: Supported by the National Natural Science Foundation of China (59675080, 59805006) and Wuhan Chenguang Foundation (20025001001)Biography: Zhang Xin-bao (1965-), male, Associate professor, research direction: precise mechanism and instrument.展开更多
基金Th is study was supported by the National Natural Science Foundation of China(NSFC,no.40172056)the Research Fund for the Doctoral Program of Higher Education,China(RFDP,no.2000042506).
文摘With a production of 208.2 m3/d, heavy oil was produced by drill stem test (DST) from three shallow reservoirs in Sand Group Nos. Ⅰ and Ⅲ of the Neogene Guantao Formation (NgⅠ and NgⅢ) and the Eogene Dongying Formation (Ed) in an exploratory well Ban-14-1 within the Qianmiqiao region, Bohai Bay Basin, northern China. Based on the GC and GC-MS data of the NgⅠ and NgⅢ heavy oil samples, all n-alkanes and most isoprenoid hydrocarbons are lost and the GC baseline appears as an evident 'hump', implying a large quantity of unresolved complex mixture (UCM), which typically revealed a result of heavy biodegradation. However, there still is a complete series of C14-C73 n-alkanes in the high-temperature gas chromatograms (HTGC) of the heavy oil, among which, the abundance of C30- n-alkanes are drastically reduced. The C35-C55 high molecular weight (HMW) n-alkanes are at high abundance and show a normal distribution pattern with major peak at C43 and an obvious odd-carbon-number predominance with CPI37-55 and OEP45-49 values of 1.17 and 1.16-1.20, respectively. According to GC-MS analysis, the heavy oil is characterized by dual source inputs of aquatic microbes and terrestrial higher plants. Various steranes and tricyclic terpanes indicate an algal origin, and hopane-type triterpanes, C24 tetracyclic terpane and drimane series show the bacterial contribution. With the odd-carbon-number preference, HMW n-alkanes provide significant information not only on higher plant source input and immaturity, but also on the strong resistibility to biodegradation.
文摘A azoreductase gene with 537 bp was obtained by PCR amplification from Rhodobacter sphaeroides AS1 1737 The enzyme, with a molecular weight of 18 7 kD, was efficiently expressed in Escherichia coli and its biodegradation characteristics for azo dyes were investigated. Furthermore, the reaction kinetics and mechanism of azo dyes catalyzed by the genetically engineered azoreductase were studied in detail. The presence of a hydrazo-intermediate was identified, which provided a convincing evidence for the assumption that azo dyes were degraded via an incomplete reduction stage.
文摘Auto anti-collision technology is one of the main research subjects of automobiles’ safety technology. Aiming at the key technology of Auto anti-collision, measuring the distance from obstacles, based on the theory of phase laser distance ranging, Laser Diode (LD) distance-measuring system for auto anti-collision has been developed to solve the problem of on-line measuring distance technology in middle to long distance utilizing the good characteristics of LD when modulating its optical intensity and adopting typical kinds of filter techniques in this paper. By theoretical analysis, adopting typical kinds of filter techniques can reduce the interference of strong light, so distance-measuring range can be 0.5–100 m in daytime or 1–200 m at night. And more, from theoretical analysis and experiment result, it can guarantee the high measuring resolution which can be less than 24.5 mm, utilizing the method of two Laser Diode optical intensity modulating wavelength and complimenting precise calibration and revision. The idea of LD distance-measuring technology is novel and feasible and this technology can be applied in Auto anti-collision. Key words laser diode - phase laser distance ranging - filter techniques - auto anti-collision CLC number TH 161 Foundation item: Supported by the National Natural Science Foundation of China (59675080, 59805006) and Wuhan Chenguang Foundation (20025001001)Biography: Zhang Xin-bao (1965-), male, Associate professor, research direction: precise mechanism and instrument.
