目的:探讨情绪释放疗法(EFT)联合低血糖指数膳食对糖尿病(DM)患者心理弹性和血糖的影响。方法:选取2022年1月—2023年1月接收的DM患者80例,根据随机数字表法分为对照组和观察组,每组40例。两组均进行常规护理,基于此,对照组进行低血糖...目的:探讨情绪释放疗法(EFT)联合低血糖指数膳食对糖尿病(DM)患者心理弹性和血糖的影响。方法:选取2022年1月—2023年1月接收的DM患者80例,根据随机数字表法分为对照组和观察组,每组40例。两组均进行常规护理,基于此,对照组进行低血糖指数膳食护理,观察组进行EFT联合低血糖指数膳食护理,干预2周。于干预前、干预2周后比较两组血糖指标[空腹血糖(FBG)、餐后2 h血糖(2 h PG)]、心理状况[焦虑自评量表(SAS)、抑郁自评量表(SDS)]及心理弹性[心理弹性量表(CD-RISC)]。结果:干预2周后,两组FBG和2 h PG水平均下降,且观察组低于对照组,差异有统计学意义(P<0.05);两组SAS和SDS评分均下降,CD-RISC评分升高,且观察组优于对照组,差异有统计学意义(P<0.05)。结论:EFT联合低血糖指数膳食能够降低DM患者血糖水平,缓解负性情绪,提高心理弹性。展开更多
为解决噪声以及信号干扰等因素严重影响接收端信道估计的问题,提出一种基于集成神经网络的信道估计方法,利用多个改进后的神经网络来提取带噪导频信号与原始导频信号之间的非线性关系模型,根据其输出误差来计算差异度值,并结合差异度对...为解决噪声以及信号干扰等因素严重影响接收端信道估计的问题,提出一种基于集成神经网络的信道估计方法,利用多个改进后的神经网络来提取带噪导频信号与原始导频信号之间的非线性关系模型,根据其输出误差来计算差异度值,并结合差异度对其进行集成,得到训练完成的集成神经网络模型,通过集成神经网络模型来获取信道估计结果;与最小二乘(least squares,LS)估计方法相比,该方法不仅可以提高通信的可靠性,而且还能减少导频开销提高通信有效性;在不同的调制方式下,当误码率相同时,算法所需的信噪比LS算法均要更低;基于正交频分复用(orthogonal frequency division multiple,OFDM)通信系统进行仿真,结果证明,该方法具有优良的性能。展开更多
Isoamylase 1(ISA1) is an isoamylase-type debranching enzyme which plays a predominant role in amylopectin synthesis. In this study, the clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associat...Isoamylase 1(ISA1) is an isoamylase-type debranching enzyme which plays a predominant role in amylopectin synthesis. In this study, the clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated endonuclease 9(CRISPR/Cas9) system was used to edit ISA1 gene in rice via Agrobacterium-mediated transformation. We identified 36 genetic edited lines from 55 T_0 transgenic events, and classified the mutation forms into 7 types. Of those, two homozygous mutants, cr-isa1-1(type 1, with an adenine insertion) and cr-isa1-2(type 3, with a cytosine deletion) were selected for further analysis. Seed sizes of both cr-isa1-1 and cr-isa1-2 were affected, and the two mutants also displayed a shrunken endosperm with significantly lower grain weight. Electron microscopy analysis showed that abnormal starch granules and amyloplasts were found in cr-isa1-1 and cr-isa1-2 endosperm cells. The contents of total starch, amylose and amylopectin in the endosperm of the cr-isa1 mutants were significantly reduced, whereas sugar content and starch gel consistency were observably increased compared to the wild-type. The gelatinization temperature and starch chain length distributions of the cr-isa1 mutants were also altered. Moreover, transcript levels of most starch synthesis-related genes were significantly lower in cr-isa1 mutants. In conclusion, the results indicated that gene edition of ISA1 affected starch synthesis and endosperm development, and brought potential implications for rice quality breeding.展开更多
The brown planthopper （BPH）, Nilaparvata lugens, is a destructive insect pest of rice throughout Asia. Different from brown-eye color wild type, BPH also has red-eye color mutation phenotype. As a visible genetic ma...The brown planthopper （BPH）, Nilaparvata lugens, is a destructive insect pest of rice throughout Asia. Different from brown-eye color wild type, BPH also has red-eye color mutation phenotype. As a visible genetic marker, the red-eye mutant in BPH is a valuable material. To reveal the eye color mutation mechanism, a karmoisin homologue gene （named as Nlka） was cloned from BPH. And karmoisin is always deemd as a xanthommatin-related gene in other insects, encoding phenoxazinone synthetase （PHS）. Nlka is consisted of 7 exons and encodes a protein with 502 amino acids （NIKA）. NIKA showed high amino acid identities with its insect homologues （48.8%-51.8%）. Nlka transcripts can be detected at all the developmental stages and in all tissues tested, including egg, nymph, adult, body wall, ovary, fat body, midgut and Malpighian tubule. However, no constant In/Del or non-synonymous mutation was observed between the mutant and the wild type strains. Quantitative real-time PCR experiment also showed that Nlka transcript level had no significant differences between them. These results indicated that Nlka is not the target gene causing the red-eye color mutation phenotype of BPH. Through the second structure and motif analysis, the present study also showed that all the proteins deduced from the karmoisin genes in insects may be members of monocarboxylate transporters （MCTs） rather than PHSs.展开更多
文摘目的:探讨情绪释放疗法(EFT)联合低血糖指数膳食对糖尿病(DM)患者心理弹性和血糖的影响。方法:选取2022年1月—2023年1月接收的DM患者80例,根据随机数字表法分为对照组和观察组,每组40例。两组均进行常规护理,基于此,对照组进行低血糖指数膳食护理,观察组进行EFT联合低血糖指数膳食护理,干预2周。于干预前、干预2周后比较两组血糖指标[空腹血糖(FBG)、餐后2 h血糖(2 h PG)]、心理状况[焦虑自评量表(SAS)、抑郁自评量表(SDS)]及心理弹性[心理弹性量表(CD-RISC)]。结果:干预2周后,两组FBG和2 h PG水平均下降,且观察组低于对照组,差异有统计学意义(P<0.05);两组SAS和SDS评分均下降,CD-RISC评分升高,且观察组优于对照组,差异有统计学意义(P<0.05)。结论:EFT联合低血糖指数膳食能够降低DM患者血糖水平,缓解负性情绪,提高心理弹性。
文摘为解决噪声以及信号干扰等因素严重影响接收端信道估计的问题,提出一种基于集成神经网络的信道估计方法,利用多个改进后的神经网络来提取带噪导频信号与原始导频信号之间的非线性关系模型,根据其输出误差来计算差异度值,并结合差异度对其进行集成,得到训练完成的集成神经网络模型,通过集成神经网络模型来获取信道估计结果;与最小二乘(least squares,LS)估计方法相比,该方法不仅可以提高通信的可靠性,而且还能减少导频开销提高通信有效性;在不同的调制方式下,当误码率相同时,算法所需的信噪比LS算法均要更低;基于正交频分复用(orthogonal frequency division multiple,OFDM)通信系统进行仿真,结果证明,该方法具有优良的性能。
基金supported by the National Nature Science Foundation of China (Grant Nos. 31471472 and 31521064)the National S&T Major Project of China (Grant No. 2016ZX08001006)the Central Level, Non-Profit, Scientific Research Institutes Basic R and D Operations Special Fund (Grant Nos.Y2017PT46 and 2017RG002-1)
文摘Isoamylase 1(ISA1) is an isoamylase-type debranching enzyme which plays a predominant role in amylopectin synthesis. In this study, the clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated endonuclease 9(CRISPR/Cas9) system was used to edit ISA1 gene in rice via Agrobacterium-mediated transformation. We identified 36 genetic edited lines from 55 T_0 transgenic events, and classified the mutation forms into 7 types. Of those, two homozygous mutants, cr-isa1-1(type 1, with an adenine insertion) and cr-isa1-2(type 3, with a cytosine deletion) were selected for further analysis. Seed sizes of both cr-isa1-1 and cr-isa1-2 were affected, and the two mutants also displayed a shrunken endosperm with significantly lower grain weight. Electron microscopy analysis showed that abnormal starch granules and amyloplasts were found in cr-isa1-1 and cr-isa1-2 endosperm cells. The contents of total starch, amylose and amylopectin in the endosperm of the cr-isa1 mutants were significantly reduced, whereas sugar content and starch gel consistency were observably increased compared to the wild-type. The gelatinization temperature and starch chain length distributions of the cr-isa1 mutants were also altered. Moreover, transcript levels of most starch synthesis-related genes were significantly lower in cr-isa1 mutants. In conclusion, the results indicated that gene edition of ISA1 affected starch synthesis and endosperm development, and brought potential implications for rice quality breeding.
基金supported by the National Natural Science Foundation of China (Grant Nos. 31201511 and 31301661)the Special Fund for Basic Expenditure for Scientific & Research of Central Non-Profit Scientific Research Institutions in China (Grant No. 2014RG005-3)
文摘The brown planthopper （BPH）, Nilaparvata lugens, is a destructive insect pest of rice throughout Asia. Different from brown-eye color wild type, BPH also has red-eye color mutation phenotype. As a visible genetic marker, the red-eye mutant in BPH is a valuable material. To reveal the eye color mutation mechanism, a karmoisin homologue gene （named as Nlka） was cloned from BPH. And karmoisin is always deemd as a xanthommatin-related gene in other insects, encoding phenoxazinone synthetase （PHS）. Nlka is consisted of 7 exons and encodes a protein with 502 amino acids （NIKA）. NIKA showed high amino acid identities with its insect homologues （48.8%-51.8%）. Nlka transcripts can be detected at all the developmental stages and in all tissues tested, including egg, nymph, adult, body wall, ovary, fat body, midgut and Malpighian tubule. However, no constant In/Del or non-synonymous mutation was observed between the mutant and the wild type strains. Quantitative real-time PCR experiment also showed that Nlka transcript level had no significant differences between them. These results indicated that Nlka is not the target gene causing the red-eye color mutation phenotype of BPH. Through the second structure and motif analysis, the present study also showed that all the proteins deduced from the karmoisin genes in insects may be members of monocarboxylate transporters （MCTs） rather than PHSs.
