目的探讨妊娠合并重症急性胰腺炎(severe acute pancreatitis in pregnancy,SAPIP)的临床特征、母儿不良结局及防治措施,为提高诊疗技术水平及降低母儿不良结局的发生提供参考依据。方法回顾性分析2001年1月1日至2020年12月31日大连医...目的探讨妊娠合并重症急性胰腺炎(severe acute pancreatitis in pregnancy,SAPIP)的临床特征、母儿不良结局及防治措施,为提高诊疗技术水平及降低母儿不良结局的发生提供参考依据。方法回顾性分析2001年1月1日至2020年12月31日大连医科大学附属第一医院收治的21例妊娠合并SAPIP患者临床资料,包括年龄、孕周、孕产次、发病时间、病因、临床表现、实验室指标、影像学检查、诊断、治疗、妊娠结局、胎儿结局等。根据孕妇是否发生多器官功能障碍分组,比较孕妇并发多器官功能障碍综合征及未并发多器官功能障碍综合征组间各临床指标的差异。根据是否发生死胎分组,比较胎儿死亡组与胎儿存活组间临床资料的差异。结果21例SAPIP患者均出现重症胰腺炎相关临床表现,影像学检查均提示胰腺炎。其中9例有高脂饮食史,入院初行腹部B超检查的16例患者中有14例表现为胆石症并发急性胰腺炎,21例患者入院后的实验室检查结果中血清甘油三酯平均水平(12.87±7.15)mmol/L,符合高脂血症诊断标准。21例SAPIP患者中10例发生多器官功能障碍综合征。并发多器官功能障碍综合征组白细胞总数、代谢性酸中毒及高脂血症发生率明显高于未并发多器官功能障碍综合征组,差异有统计学意义(P<0.05);并发多器官功能障碍综合征组血钙浓度明显低于未并发多器官功能障碍综合征组,差异有统计学意义(P<0.05)。除去人工流产2例,19例患者中3例胎死宫内,16例胎儿存活。胎儿死亡组多器官功能障碍综合征、失代偿性代谢性酸中毒发生率、白细胞总数,明显高于胎儿存活组,差异有统计学意义(P<0.05);血钙浓度、动脉血气碱剩余,明显低于胎儿存活组,差异有统计学意义(P<0.05)。结论高脂血症及胆道系统疾病是SAPIP的主要诱发因素,临床指标中血钙浓度降低、白细胞总数升高及代谢性酸中毒提示发生多器官功能障碍综合征及死胎的风险增加。展开更多
Spermatogenesis is a complicated and poorly understood process that relies on the precise regulation of the self-renewal and differentiation of spermatogonia. In many organisms, micro RNAs(mi RNAs) are involved in mul...Spermatogenesis is a complicated and poorly understood process that relies on the precise regulation of the self-renewal and differentiation of spermatogonia. In many organisms, micro RNAs(mi RNAs) are involved in multiple developmental processes as critical regulators of transcriptional and post-transcriptional gene silencing. This study investigated the expression pattern of mi RNAs in type B spermatogonia cells(BSc) and primary spermatocytes(PSc) of mice, using a high-throughput small RNA sequencing system. The results revealed that the expression levels of Let-7 family mi RNAs were remarkably high in both cell types. Furthermore, the expression levels of mi R-21, mi R-140-3p, mi R-103, mi R-30 a, mi R-101 b and mi R-99 b were decreased during the transformation from BSc to PSc. These mi RNAs target vital genes that participate in apoptosis, cell proliferation and differentiation, junction assembly and cell cycle regulation. These results highlight the indispensable role of mi RNAs in spermatogenesis.展开更多
文摘目的探讨妊娠合并重症急性胰腺炎(severe acute pancreatitis in pregnancy,SAPIP)的临床特征、母儿不良结局及防治措施,为提高诊疗技术水平及降低母儿不良结局的发生提供参考依据。方法回顾性分析2001年1月1日至2020年12月31日大连医科大学附属第一医院收治的21例妊娠合并SAPIP患者临床资料,包括年龄、孕周、孕产次、发病时间、病因、临床表现、实验室指标、影像学检查、诊断、治疗、妊娠结局、胎儿结局等。根据孕妇是否发生多器官功能障碍分组,比较孕妇并发多器官功能障碍综合征及未并发多器官功能障碍综合征组间各临床指标的差异。根据是否发生死胎分组,比较胎儿死亡组与胎儿存活组间临床资料的差异。结果21例SAPIP患者均出现重症胰腺炎相关临床表现,影像学检查均提示胰腺炎。其中9例有高脂饮食史,入院初行腹部B超检查的16例患者中有14例表现为胆石症并发急性胰腺炎,21例患者入院后的实验室检查结果中血清甘油三酯平均水平(12.87±7.15)mmol/L,符合高脂血症诊断标准。21例SAPIP患者中10例发生多器官功能障碍综合征。并发多器官功能障碍综合征组白细胞总数、代谢性酸中毒及高脂血症发生率明显高于未并发多器官功能障碍综合征组,差异有统计学意义(P<0.05);并发多器官功能障碍综合征组血钙浓度明显低于未并发多器官功能障碍综合征组,差异有统计学意义(P<0.05)。除去人工流产2例,19例患者中3例胎死宫内,16例胎儿存活。胎儿死亡组多器官功能障碍综合征、失代偿性代谢性酸中毒发生率、白细胞总数,明显高于胎儿存活组,差异有统计学意义(P<0.05);血钙浓度、动脉血气碱剩余,明显低于胎儿存活组,差异有统计学意义(P<0.05)。结论高脂血症及胆道系统疾病是SAPIP的主要诱发因素,临床指标中血钙浓度降低、白细胞总数升高及代谢性酸中毒提示发生多器官功能障碍综合征及死胎的风险增加。
基金supported by the National Natural Science Foundation of China(81170616,81072093,30671092,81302323,31100915)the Natural Science Foundation of Hebei Province(C2014209140,C2009001260,C2012401039,H2013209194,C2013209024)the Scientific and Technical Supporting Programs of Hebei Province(10276109D)
文摘Spermatogenesis is a complicated and poorly understood process that relies on the precise regulation of the self-renewal and differentiation of spermatogonia. In many organisms, micro RNAs(mi RNAs) are involved in multiple developmental processes as critical regulators of transcriptional and post-transcriptional gene silencing. This study investigated the expression pattern of mi RNAs in type B spermatogonia cells(BSc) and primary spermatocytes(PSc) of mice, using a high-throughput small RNA sequencing system. The results revealed that the expression levels of Let-7 family mi RNAs were remarkably high in both cell types. Furthermore, the expression levels of mi R-21, mi R-140-3p, mi R-103, mi R-30 a, mi R-101 b and mi R-99 b were decreased during the transformation from BSc to PSc. These mi RNAs target vital genes that participate in apoptosis, cell proliferation and differentiation, junction assembly and cell cycle regulation. These results highlight the indispensable role of mi RNAs in spermatogenesis.
