Study on the mechanism of effect of Daodi Tongguan Decoction on rats with premature ovarian failure

Objective:To investigate the mechanism of the effect of Daodi Tongguan Decoction on ovarian function in rats with premature ovarian failure.Methods:Forty SD female rats with regular estrous cycle were randomly divided into the blank group,the model group,the positive group(Bujiale 0.099 mg/kg),the Daodigitongguan decoction high-dose group(1.4 g/100 g)and the Daodigitongguan decoction medium-dose group(0.7 g/100 g).The model group,positive group,Daodi Tongguan Decoction high-dose group and Daodi Tongguan decoction medium-dose group were given Tripterygium wilfordii polyglycoside tablets suspension(50 mg/kg)by continuous gavage for 14 days to establish a rat model of premature ovarian failure.After the modeling,each group was given the corresponding intervention agent for 28 consecutive days.After the intervention,serum and ovarian tissue were collected from rats in each group.The morphological structure of rat ovarian tissue was observed after HE staining.Serum AMH level was determined by ELISA.The expressions of Bcl-2,Bax and VEGF in ovarian tissue were detected by IHC.The apoptosis of granulosa cells in ovary was observed by TUNEL.Results:Compared to the normal group,the growth follicles in the model group were reduced and the atretic follicles were increased.Serum AMH level was significantly decreased(P<0.01).The expression of Bax protein in ovarian tissue was increased(P<0.01).The protein expressions of Bcl-2 and VEGF were decreased(P<0.01,P<0.05),and the apoptosis rate of follicular granulosa cells in ovarian tissue was increased(P<0.01).Compared to the molding group,the serum AMH level in the TCM dose groups was increased to varying degrees,and the change of the AMH in high-dose group was the most significant(P<0.05).The expression of Bax protein in ovarian tissue decreased(P<0.05),while the expression of Bcl-2 and VEGF protein increased(P<0.05,P<0.05).The apoptosis rate of follicular granulosa cells in ovarian tissue was decreased in the high dose group(P<0.05).Conclusion:Daodi Tongguan Decoction can improve the histological morphology of ovary and relieve ovarian injury,and its mechanism may be related to improving the sex hormone level of POF model rats,reducing the expression level of Bax protein in ovarian tissue,increasing the expression level of Bcl-2 and VEGF protein in ovarian tissue,and reducing the apoptosis rate of follicular granulosa cells in ovarian tissue.

桂罗氏参补汤治疗出血期气虚血瘀型崩漏的临床疗效

目的探讨桂罗氏参补汤(简称"参补汤")治疗出血期气虚血瘀型崩漏的临床疗效。方法将120例出血期气虚血瘀型崩漏患者随机分为观察组和对照组,每组60例。观察组给予参补汤口服,对照组给予伊血安颗粒口服。比较两组患者治疗前后的中医症状积分、治疗后的止血疗效及中医证候疗效、治疗期间不良反应发生情况。结果观察组近期止血疗效和中医证候疗效均优于对照组(均P <0. 05)。治疗后,两组中医症状积分均较治疗前下降(均P <0. 05),且观察组低于对照组(P <0. 05)。两组均无严重不良反应发生。结论参补汤对气虚血瘀型崩漏出血期患者的止血疗效较好,并能改善其伴随症状,安全性较好。

广西名中医罗志娟教授“三分法”治疗高龄女性不孕症经验浅析

随着国家二孩政策的放开,高龄生育问题日益突出,女性生育能力因年龄的增加而下降,因此不孕症发生率也随之增加。现从对疾病的认识、辩证、“三分法”治疗以及“三主张,一建议”等方面总结罗志娟教授治疗高龄不孕临床经验。认为本病发生主要以肾虚为本,心、肝、脾脏腑功能失调为要,痰湿血瘀为主要病理产物,共同导致肾-天癸-冲任-胞宫生殖轴功能紊乱而发生不孕。

“三分法”辨治早发性卵巢功能不全的经验探讨

总结罗志娟教授辨治早发性卵巢功能不全的临床经验,早发性卵巢功能不全的病理基础是肾虚,诊疗过程中须明确诊断,早治早防;治疗上以“补肾”为基础,倡导“三分法”,即“分阶段”、“分周期”、“分证型”遣方用药,灵活加减治疗早发性卵巢功能不全;并善用膏方,以平为期,临床疗效显著。

罗志娟“通补清消”法辨治月经过多探讨

从对疾病的认识、辨证、治法、用方总结罗志娟教授治疗月经过多的临床经验,认为月经过多主要病机是冲任不固、经血失于制约,常见的病因是气虚、血热以及血瘀;若病程日久,气随血耗,致气更虚,气虚运血无力,瘀血内阻,瘀阻日久化热,热迫血行,致虚、热、瘀交织,虚实夹杂互为因果而致病,故'气虚证''血热证''血瘀证''气虚血瘀证'为月经期四大证型且临床常见多证型合并;治疗以'通补清消'立法,对于常见证型'气虚血瘀证'巧用'通补'治法,寓通于补,以通为用;用方上,以自拟参补汤为基础方结合补肾调经治本之1、2、3号方加减分月经期、非经期治疗疗效显著。

罗志娟“桂罗氏三分法”辨治经行头痛经验

罗志娟教授针对经行头痛的特点,采用"桂罗氏三分法"治疗,即根据头痛患者的证型、疾病发展的不同阶段及所处的月经周期,"分证""分阶段""分周期"立方遣药。"分证"为以局部辨证定病变脏腑经络,以整体辨证定头痛的虚实寒热。经行头痛基本病机有二,一是气血失调,一是冲气上逆,合而致病,缺一不可。实证者,行气活血以止痛;虚证者,补气养血以止痛。无论何证,均注重平冲安冲,从肝脾肾三脏从发,结合证型,或补肾固冲,或平肝降逆,或健脾和中。"分阶段"为将经行头痛分为痛止期与疼痛期,痛止期,以治本为主,方药根据所属证型不同,或益气养血,或滋阴潜阳,或活血化瘀,或燥湿化痰;疼痛期,常用川芎茶调散加天麻、延胡索,以急治其标,缓解疼痛。"分周期"为将经行头痛分为三期治疗,行经期,以除旧为主;经后期,滋阴养血,佐以助阳;经前期,补肾助阳,扶助阳长。三分的结果需合而互参,不可有所偏颇,分周期、分阶段亦要针对各种证型进行辨证加减。

18%草铵膦防治柑桔园杂草田间药效试验

由于41%草甘膦在江西省永修县使用多年,本地多种杂草对其产生抗药性。18%草铵膦每667 m^2 200 mL用量对桔园多种抗性杂草5 d后就可达到满意效果,对牛筋草的株防效,2,5,10,30 d分别可达61.03%,78.72%,81.98%,84.86%,30 d鲜质量防效92.91%;对小蓬草的株防效,2,5,10,30 d分别为41.67%,86.36%,100%,10～30 d的鲜质量防效均为100%。