高校有组织科研的界面治理:理论逻辑与实践路径

在大科学时代背景下,高校有组织科研作为回应国家战略发展需求与驱动原始性、引领性科技创新的重要方式,展现为多维交织、跨界协同的复杂系统工程,其核心要义在于实现多主体和多要素的协同联动,强调科研的科学治理和范式重塑。将界面治理理论分析框架嵌入高校有组织科研实践,通过纵向、横向、斜向三个维度的深入剖析,阐释高校有组织科研的界面生成、功能与目标、内部结构、外部情境等核心构成要素,厘清高校有组织科研的内在逻辑。深化高校有组织科研的治理路径应分别从价值目标、理念共识、制度驱动和跨界协同等四个方面着手,进一步夯实黏合性界面治理体系,构建弹性化界面管理机制,形成渗透性界面治理结构,打造交互性界面治理网络,融合跨领域、跨学科、跨部门、跨空间的治理界面。

UPLC法测定心舒宝片中11种酚酸类成分的含量

目的:建立超高效液相色谱法(UPLC)测定心舒宝片中11种酚酸类成分的含量,为心舒宝片的质量评价奠定基础。方法:采用Welch Ultimate XB-C18(2.1 mm×150 mm,1.8μm)色谱柱;柱温为25℃;梯度洗脱;流速0.2 mL·min^(-1);检测波长为320 nm;进样量为3μL。利用UPLC法对心舒宝片中包括没食子酸、丹参素、原儿茶酸、新绿原酸、原儿茶醛、绿原酸、隐绿原酸、迷迭香酸、紫草酸、丹酚酸B、丹酚酸A等11种化学成分进行定量分析。结果:在考察的浓度范围内,各化学成分均具有良好的线性关系(r>0.9994),回收率均在98.37%~101.15%,RSD在1.20%~2.81%,且精密度、稳定性和重复性均符合分析要求。结论:本实验采用UPLC法高效、快速、全面地对心舒宝片的11种质量标志物进行分析与含量测定,为心舒宝片药效物质基础研究及生物效应质量评价奠定基础。

QuEChERS法检测植物提取类化妆品中有机氯农药残留

建立检测植物提取类化妆品中有机氯农药残留的方法。采用QuEChERS法优化前处理,经气相色谱质谱联用仪检测分析结果。优化后采用乙腈溶剂,加入2 g氯化钠、3 g无水硫酸镁、1 g柠檬酸钠、0.5 g柠檬酸氢二钠进行提取,净化剂:150 mg MgSO_(4)、50 mg PSA、80 mg C_(18)。结果18种有机氯农药的线性范围为0.05~0.5 mg/kg,线性相关系数均≥0.995,检出限为0.006~0.059 mg/kg,定量限为0.018~0.190 mg/kg。在0.1,0.2,0.5 mg/L三个浓度下加标回收率在75.23%~107.49%之间,相对标准偏差RSD在2.37%~6.31%之间。该方法操作简单、快速、稳定,适用于高通量农药残留检测。

Pien Tze Huang Inhibits Migration and Invasion of Hepatocellular Carcinoma Cells by Repressing PDGFRB/YAP/CCN2 Axis Activity

Objective:To investigate the effects of Pien Tze Huang(PZH) on the migration and invasion of HCC cells and underlying molecular mechanism.Methods:Cell counting kit-8(CCK-8) was applied to evaluate the cell viabilities of SMMC-7721,SK-Hep-1,C3A and HL-7702(6 × 10^(3)cells/well) co-incubated with different concentrations of PZH(0,0.2,0.4,0.6,0.8 mg/mL) for 24 h.Transwell,wound healing assay,CCK-8and Annexin V-FITC/PI staining were conducted to investigate the effects of PZH on the migration,invasion,proliferation and apoptosis of SK-Hep-1 and SMMC-7721 cells(650 μg/mL for SK-Hep-1 cells and 330 μg/mL for SMMC-7721 cells),respectively.In vivo,lung metastasis mouse model constructed by tail vein injection of HCC cells was used for evaluating the anti-metastasis function of PZH.SK-Hep-1 cells(10^(6)cells/200 μL per mice) were injected into B-NDG mice via tail vein.Totally 8 mice were randomly divided into PZH and control groups,4 mice in each group.After 2-d inoculation,mice in the PZH group were administered with PZH(250 mg/kg,daily) and mice in the control group received only vehicle(PBS) from the 2nd day after xenograft to day 17.Transcriptome analysis based on RNA-seq was subsequently used for deciphering anti-tumor mechanism of PZH.Quantitative real-time polymerase chain reaction(qRT-PCR) and Western blot were applied to verify RNA-seq results.Luciferase reporter assay was performed to examine the transcriptional activity of yes-associated protein(YAP).Results:PZH treatment significantly inhibited the migration,invasion,proliferation and promoted the apoptosis of HCC cells in vitro and in vivo(P<0.01).Transcriptome analysis indicated that Hippo signaling pathway was associated with anti-metastasis function of PZH.Mechanical study showed PZH significantly inhibited the expressions of platelet derived growth factor receptor beta(PDGFRB),YAP,connective tissue growth factor(CCN2),N-cadherin,vimentin and matrix metallopeptidase 2(MMP2,P<0.01).Meanwhile,the phosphorylation of YAP was also enhanced by PZH treatment in vitro and in vivo.Furthermore,PZH played roles in inhibiting the transcriptional activity of YAP.Conclusion:PZH restrained migration,invasion and epithelialmesenchymal transition of HCC cells through repressing PDGFRB/YAP/CCN2 axis.

Functional polymorphism in exon 5 and variant haplotype of the interleukin-1 receptor-associated kinase 1 gene are associated with susceptibility to and severity of sepsis in the Chinese population

Background The interleukin-1 （IL-1） receptor-associated kinase 1 （IRAK1） is believed to play an important role in the pathogenesis of sepsis. Recent studies have suggested that the I RAK1 functional genetic variant could affect the severity of sepsis in Caucasians. In this report, we have investigated whether polymorphisms at the IRAK1 gene are associated with the susceptibility to and severity of sepsis among the Chinese population. Methods Haplotype-tagging single nucleotide polymorphisms （htSNPs） were selected from the HapMap database. They.were genotyped in 255 patients with sepsis and 260 control subjects by PCR/restriction fragment length polymorphism （RFLP） analysis. The association between the selected htSNPs and the susceptibility to and severity of sepsis were estimated by Logistic regression with adjustments for age, sex, smoking, drinking, chronic disease status, Acute Physiology and Chronic Health Evaluation （APACHE） II score and primary diseases. Results rs1059702 was selected to represent the six linked htSNPs for IRAKI. Genotype frequencies of the htSNPs were in Hardy-Weinberg equilibrium for females, as were allele frequencies for both sex groups. Associations were observed in females between the htSNPs C/C genotype and increased susceptibility to sepsis （odds ratio （OR）, 5.46; 95% confidence interval （C/）, 1.12-26.67; P=0.018）, and such associations were also observed between the IRAK1 variant haplotype （CC/C-allele） and increased susceptibility to sepsis （OR, 1.68; 95% C/, 1.05-2.70; P=0.031） when compared with the T/T ＋ T/C genotype and the wild-type haplotype （TC ＋ TT/T-allele）. In the multiple organ dysfunction syndrome （MODS） subgroup, the variant haplotype was also associated with increased severity of sepsis （OR, 2.37; 95% Cl, 1.13-4.94; P=-0.02） when compared with the wild haplotype. This association was not significant in male patients. Conclusions The functional polymorphism in exon 5 and the variant haplotype of IRAK1 gene mediate susceptibility to and severity of sepsis. IRAK1 might be a genetic risk factor for the occurrence and development of sepsis in the Chinese population.