OBJECTIVE:To evaluate the effects of Zhenggan Huayu decoction(正肝化瘀方,ZGHY) combined with entecavir(ETV) on the gut microbiota in patients with chronic hepatitis B(CHB) fibrosis.METHODS:A total of 59 CHB-related fi...OBJECTIVE:To evaluate the effects of Zhenggan Huayu decoction(正肝化瘀方,ZGHY) combined with entecavir(ETV) on the gut microbiota in patients with chronic hepatitis B(CHB) fibrosis.METHODS:A total of 59 CHB-related fibrosis patients were enrolled and treated with ZGHY combined with ETV(ZGHY + ETV) and ETV alone.Fecal samples were collected from patients at weeks 0,12,and 24 after treatment and gut microbiota were analyzed by 16S r RNA gene sequencing.RESULTS:Compared to the ETV group,microbiota diversity in the ZGHY + ETV group was increased after 24 weeks.Some potentially pathogenic bacteria,including Fusobacteriaceae spp.,Acidaminococcus spp.,and Erysipelotrichaceae spp.were reduced in the ZGHY + ETV group,while Eubacteriaceae spp.,Lactococcus spp.,and several other beneficial bacteria were increased.CONCLUSION:Decreases in pathogenic bacteria and increases in probiotics were not always observed in the Traditional Chinese Medicine(TCM) group(e.g.,Rothia was abundant).As an adjuvant TCM formulation for ETV,ZGHY had a positive role in the treatment of CHB patients.展开更多
基金Supported by Shenzhen Science and Technology Plan Project:Study on the Mechanism of Zhenggan Huayu Decoction Regulating Intestinal Flora in Liver Fibrosis based on LPS-TRL4 Signal Pathway(No.JCYJ20180302150216419)。
文摘OBJECTIVE:To evaluate the effects of Zhenggan Huayu decoction(正肝化瘀方,ZGHY) combined with entecavir(ETV) on the gut microbiota in patients with chronic hepatitis B(CHB) fibrosis.METHODS:A total of 59 CHB-related fibrosis patients were enrolled and treated with ZGHY combined with ETV(ZGHY + ETV) and ETV alone.Fecal samples were collected from patients at weeks 0,12,and 24 after treatment and gut microbiota were analyzed by 16S r RNA gene sequencing.RESULTS:Compared to the ETV group,microbiota diversity in the ZGHY + ETV group was increased after 24 weeks.Some potentially pathogenic bacteria,including Fusobacteriaceae spp.,Acidaminococcus spp.,and Erysipelotrichaceae spp.were reduced in the ZGHY + ETV group,while Eubacteriaceae spp.,Lactococcus spp.,and several other beneficial bacteria were increased.CONCLUSION:Decreases in pathogenic bacteria and increases in probiotics were not always observed in the Traditional Chinese Medicine(TCM) group(e.g.,Rothia was abundant).As an adjuvant TCM formulation for ETV,ZGHY had a positive role in the treatment of CHB patients.
