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Hippo信号通路中MST1和MST2的表达在肝癌干细胞干性维持和自我更新中的意义 被引量:1
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作者 闾少冬 魏勇鹏 +2 位作者 王卓 袁建勇 卢军华 《肝脏》 2021年第8期866-870,共5页
目的初步探索Hippo信号通路中MST1和MST2的表达在肝癌干细胞干性维持和自我更新中的作用。方法qRT-PCR和免疫组化检测肝癌组织样本中MST1和MST2的表达;qRT-PCR和Western blot检测肝癌细胞中MST1和MST2的表达;通过流式细胞术筛选CD90+、C... 目的初步探索Hippo信号通路中MST1和MST2的表达在肝癌干细胞干性维持和自我更新中的作用。方法qRT-PCR和免疫组化检测肝癌组织样本中MST1和MST2的表达;qRT-PCR和Western blot检测肝癌细胞中MST1和MST2的表达;通过流式细胞术筛选CD90+、CD105+、CD133+型的SMMC-7721细胞;qRT-PCR和Western blot检测干细胞标志物水平;转染shRNA在肝癌干细胞中敲低MST1和MST2的表达,镜检观察细胞微球体形成情况,并通过qRT-PCR和Western blot检测干细胞标志物水平。结果MST1和MST2在肝癌组织样本和肝癌细胞中显著高表达;CD90+、CD105+、CD133+型的SMMC-7721细胞微球体形成能力显著增强,干细胞标志物AMPK磷酸化的水平增加,OCT-4和Nanog的表达上调为2.56±0.21、2.32±0.11;敲低MST1和MST2都能显著CD90+、CD105+、CD133+型的SMMC-7721细胞的微球体形成能力,干细胞标志物AMPK磷酸化的水平减弱,敲低MST1后,OCT-4和Nanog的表达下调为0.48±0.02和0.26±0.08;敲低MST1后,OCT-4和Nanog的表达下调为0.35±0.06和0.42±0.03。结论MST1和MST2的表达在肝癌干细胞的干性维持和自我更新中有着重要的生物学意义。 展开更多
关键词 MST1 MST2 肝癌 干性维持 自我更新
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Design of Objects Tracking System Based on ARM Embedded Platform 被引量:1
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作者 XU Mei SONG Yong-duan +2 位作者 lv shao-dong LIU Zhi-long HUANG Cong-ying 《Computer Aided Drafting,Design and Manufacturing》 2014年第3期65-69,共5页
In recent years, according to the need of intelligent video surveillance system increasing rapidly in metropolitan cities ,a design based on $3C2440 microprocessor and embedded Linux operating system is adopted for re... In recent years, according to the need of intelligent video surveillance system increasing rapidly in metropolitan cities ,a design based on $3C2440 microprocessor and embedded Linux operating system is adopted for real-time video target tracking. However, it is very challenging as embedded systems usually afford limited processing power and limited resources. Therefore, to address this problem, a real-time tracking algorithm using multi-features based on compressive sensing is proposed and implemented The algorithm uses multiple matrix as the projection matrix of the compressive sensing and the compressed date as the multiple features to extract useful information needed by tracking process. Functions and libraries in OpenCV which were developed by Intel Corporation are utilized for building the tracking algorithms. It is tested with variant video sequences and the results show that the algorithm achieves stable tracking for the target moved of the light changed. 展开更多
关键词 embedded system OPENCV tracking algorithms compressive sensing
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