Objective:To detect and characterize Chlamydophila psittaci(C.psittaci) in asymptomatic feral pigeons in central Thailand.Methods:A total 814 swabs from the trachea and cloacae of 407non-clinical feral pigeons in cent...Objective:To detect and characterize Chlamydophila psittaci(C.psittaci) in asymptomatic feral pigeons in central Thailand.Methods:A total 814 swabs from the trachea and cloacae of 407non-clinical feral pigeons in central Thailand were collected and tested for the presence of C.psittaci.Results:A 10.8%of feral pigeons in the sample group were positive as determined by nested PCR primer specific to C.psittaci.The outer membrane protein A(orupA) gene of positive samples exhibited amino acid identity of C.psittaci ranging from 71 to 100%and were grouped in genotype B.Exceptionally,BF1676-56 isolate was closely related to Chlamydia avium with99%identification of the I6 S ribosomal(r) RNA gene.Conclusions:This is the first report on C.psittaci isolated from asymptomatic feral pigeons in Thailand,which provides knowledge for the disease status in pigeon populations in Thailand.展开更多
Salivary glands(SG)are exocrine organs with secretory units commonly injured by radiotherapy.Bio-engineered organoids and extracellular vesicles(EV)are currently under investigation as potential strategies for SG repa...Salivary glands(SG)are exocrine organs with secretory units commonly injured by radiotherapy.Bio-engineered organoids and extracellular vesicles(EV)are currently under investigation as potential strategies for SG repair.Herein,three-dimensional(3D)cultures of SG functional organoids(SGo)and human dental pulp stem cells(hDPSC)were generated by magnetic 3D bioassembly(M3DB)platforms.Fibroblast growth factor 10(FGF10)was used to enrich the SGo in secretory epithelial units.After 11 culture days via M3DB,SGo displayed SG-specific acinar epithelial units with functional properties upon neurostimulation.To consistently develop 3D hDPSC in vitro,3 culture days were sufficient to maintain hDPSC undifferentiated genotype and phenotype for EV generation.EV isolation was performed via sequential centrifugation of the conditioned media of hDPSC and SGo cultures.EV were characterized by nanoparticle tracking analysis,electron microscopy and immunoblotting.EV were in the exosome range for hDPSC(diameter:88.03±15.60 nm)and for SGo(123.15±63.06 nm).Upon ex vivo administration,exosomes derived from SGo significantly stimulated epithelial growth(up to 60%),mitosis,epithelial progenitors and neuronal growth in injured SG;however,such biological effects were less distinctive with the ones derived from hDPSC.Next,these exosome biological effects were investigated by proteomic arrays.Mass spectrometry profiling of SGo exosomes predicted that cellular growth,development and signaling was due to known and undocumented molecular targets downstream of FGF10.Semaphorins were identified as one of the novel targets requiring further investigations.Thus,M3DB platforms can generate exosomes with potential to ameliorate SG epithelial damage.展开更多
基金financially supported by the Monitoring and Surveillance Center for Zoonotic Diseases in Wildlife and Exotic Animals.Faculty of Veterinary Science.Mahidol University
文摘Objective:To detect and characterize Chlamydophila psittaci(C.psittaci) in asymptomatic feral pigeons in central Thailand.Methods:A total 814 swabs from the trachea and cloacae of 407non-clinical feral pigeons in central Thailand were collected and tested for the presence of C.psittaci.Results:A 10.8%of feral pigeons in the sample group were positive as determined by nested PCR primer specific to C.psittaci.The outer membrane protein A(orupA) gene of positive samples exhibited amino acid identity of C.psittaci ranging from 71 to 100%and were grouped in genotype B.Exceptionally,BF1676-56 isolate was closely related to Chlamydia avium with99%identification of the I6 S ribosomal(r) RNA gene.Conclusions:This is the first report on C.psittaci isolated from asymptomatic feral pigeons in Thailand,which provides knowledge for the disease status in pigeon populations in Thailand.
基金This research project was supported by:a postdoctoral scholarship grant to A.C.from The Second Century Fund(C2F),Chulalongkorn Universitya Mid-career Research Grant to J.N.F.from the National Research Council of Thailand(NRCT5-RSA63001-12)+3 种基金a training support grant to C.A.from the National Medical Research Council Singapore(NMRC/CNIG/1131/2015)a research grant to J.N.F./R.C./S.Y.for the Avatar Biotechnologies for Oral Health and Healthy Longevity Research Unit,from Ratchadaphiseksomphot Endowment Fund at Chulalongkorn University(33/2565:RU)an internal grant from the Faculty of Dentistry Chulalongkorn University,grant number DRF 65001 to J.N.F.,S.Y.and R.C.and a grant from Mahidol University(Basic Research Fund:fiscal year 2021)to S.R..We would like to give a special thanks to Oral Biology Research Center,Faculty of Dentistry,Chulalongkorn University,Dr.Chatvadee Kornsuthisopon for providing the hDPSC conditioned media for western blotting and to Dr.Muttarin Lothong for all technical assistance.
文摘Salivary glands(SG)are exocrine organs with secretory units commonly injured by radiotherapy.Bio-engineered organoids and extracellular vesicles(EV)are currently under investigation as potential strategies for SG repair.Herein,three-dimensional(3D)cultures of SG functional organoids(SGo)and human dental pulp stem cells(hDPSC)were generated by magnetic 3D bioassembly(M3DB)platforms.Fibroblast growth factor 10(FGF10)was used to enrich the SGo in secretory epithelial units.After 11 culture days via M3DB,SGo displayed SG-specific acinar epithelial units with functional properties upon neurostimulation.To consistently develop 3D hDPSC in vitro,3 culture days were sufficient to maintain hDPSC undifferentiated genotype and phenotype for EV generation.EV isolation was performed via sequential centrifugation of the conditioned media of hDPSC and SGo cultures.EV were characterized by nanoparticle tracking analysis,electron microscopy and immunoblotting.EV were in the exosome range for hDPSC(diameter:88.03±15.60 nm)and for SGo(123.15±63.06 nm).Upon ex vivo administration,exosomes derived from SGo significantly stimulated epithelial growth(up to 60%),mitosis,epithelial progenitors and neuronal growth in injured SG;however,such biological effects were less distinctive with the ones derived from hDPSC.Next,these exosome biological effects were investigated by proteomic arrays.Mass spectrometry profiling of SGo exosomes predicted that cellular growth,development and signaling was due to known and undocumented molecular targets downstream of FGF10.Semaphorins were identified as one of the novel targets requiring further investigations.Thus,M3DB platforms can generate exosomes with potential to ameliorate SG epithelial damage.
