期刊文献+
共找到1篇文章
< 1 >
每页显示 20 50 100
Hepatocyte transplantation program:Lessons learned andfuture strategies 被引量:3
1
作者 Eugenia Pareja Ibars Miriam Cortes +4 位作者 laia tolosa Maria JoséGómez-Lechón Slivia López JoséVicente Castell JoséMir 《World Journal of Gastroenterology》 SCIE CAS 2016年第2期874-886,共13页
This review aims to share the lessons we learned over time during the setting of the hepatocyte transplantation(HT) program at the Hepatic Cell Therapy Unit at Hospital La Fe in Valencia. New sources of liver tissue f... This review aims to share the lessons we learned over time during the setting of the hepatocyte transplantation(HT) program at the Hepatic Cell Therapy Unit at Hospital La Fe in Valencia. New sources of liver tissue for hepatocyte isolation have been explored. The hepatocyte isolation and cryopreservation procedures have been optimized and quality criteria for assessment of functionality of hepatocyte preparations and suitability for HT have been established. The results indicate that:(1) Only highly viable and functional hepatocytes allow to recover those functions lacking in the native liver;(2) Organs with steatosis(≥ 40%) and from elderly donors are declined since low hepatocyte yields, viability and cell survival after cryopreservation, are obtained;(3) Neonatal hepatocytes are cryopreserved without significant loss of viability or function representing high-quality cells to improve human HT;(4) Cryopreservation has the advantage of providing hepatocytes constantly available and of allowing the quality evaluation and suitability for transplantation; and(5) Our results from 5 adults with acute liver failure and 4 from children with inborn metabolic diseases, indicate that HT could be a veryuseful and safe cell therapy, as long as viable and metabolically functional human hepatocytes are used. 展开更多
关键词 HEPATOCYTE TRANSPLANTATION Hepatocyteisolation Cell therapy INBORN errors of metabolism NEONATAL HEPATOCYTES CRYOPRESERVATION
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部