The impact of habitat fragmentation and isola-tion on the genetic diversity of populations has attracted much attention in studies of meta-population and conserva-tion biology. In this work, using the randomly amplifi...The impact of habitat fragmentation and isola-tion on the genetic diversity of populations has attracted much attention in studies of meta-population and conserva-tion biology. In this work, using the randomly amplified po-lymorphic DNA (RAPD) technique, we studied the genetic diversity of central, peripheral and peninsular populations of ratlike hamster, which were collected in five locations of the North China Plain and its surrounding areas, in 1999. The study revealed that, i ) the genetic diversity of central population of Raoyang County 】 the sub-central populations of Gu’an County and Taikang County 】 the peripheral population of Shunyi District 】 the peninsular population of Mentougou District; ii) the genetic diversities of the five populations were positively correlated to the nearest dis-tances to the peripheral line of population distribution; iii) there were significant differences of gene frequencies of some RAPD fragments among the five populations. More RAPD fragments disappeared in展开更多
Kisspeptins and G protein coupled receptor(GPR54)play significant roles in regulating reproductive activity among seasonally reproductive animals;however,the mechanisms of KiSS-1 and GPR54 gene affecting the seasonal ...Kisspeptins and G protein coupled receptor(GPR54)play significant roles in regulating reproductive activity among seasonally reproductive animals;however,the mechanisms of KiSS-1 and GPR54 gene affecting the seasonal reproduction in striped hamster are still unknown.In this study,real-time quantitative polymerase chain reaction was employed to examine the expression profiles of KiSS-1 and GPR54 in the hypothalamus,ovaries,testes,uterus and epididymis of striped hamsters across 4 different seasons.Our results showed that,across different seasons,the KiSS-1 expression mode of male striped hamsters and the GPR54 expression mode of female striped hamsters were consistent with the seasonal photoperiod in the hypothalamus.Meanwhile,across different seasons,the expression profile of KiSS-1 in the testes and the GPR54 expression profile of male striped hamsters in the hypothalamus were consistent with the intensity of their seasonal reproductive activity.Among different tissues,the expression trend for GPR54 is consistent across 4 seasons,while that for KiSS-1 is tissue-dependent.The expression trend for GPR54 across 4 seasons is the same regardless of gender,while that for KiSS-1 is dramatically different and sex-dependent across different seasons.These results suggest that the expressions of KiSS-1 and GPR54 in the striped hamsters were regulated by complicated mechanisms,and the regulatory mechanisms in the striped hamsters are seasonal-dependent and sex-dependent.This research will provide a theoretical basis for studying how KiSS-1 and GPR54 affect seasonal reproduction and the mechanisms behind their influence.展开更多
基金This work was supported by the National NaturalScience Foundation of China (Grant No. 39730090) the Key Projects of the Chinese Academy of Sciences (Grant Nos. KSCX2-1-03 and KZ951-B1-106).
文摘The impact of habitat fragmentation and isola-tion on the genetic diversity of populations has attracted much attention in studies of meta-population and conserva-tion biology. In this work, using the randomly amplified po-lymorphic DNA (RAPD) technique, we studied the genetic diversity of central, peripheral and peninsular populations of ratlike hamster, which were collected in five locations of the North China Plain and its surrounding areas, in 1999. The study revealed that, i ) the genetic diversity of central population of Raoyang County 】 the sub-central populations of Gu’an County and Taikang County 】 the peripheral population of Shunyi District 】 the peninsular population of Mentougou District; ii) the genetic diversities of the five populations were positively correlated to the nearest dis-tances to the peripheral line of population distribution; iii) there were significant differences of gene frequencies of some RAPD fragments among the five populations. More RAPD fragments disappeared in
基金supported by the National Science Foundation of China(31270417,31300304,31670385,31570377)the Provincial Key Construction Project the“twelfth five-year”plan for Qufu Normal University.
文摘Kisspeptins and G protein coupled receptor(GPR54)play significant roles in regulating reproductive activity among seasonally reproductive animals;however,the mechanisms of KiSS-1 and GPR54 gene affecting the seasonal reproduction in striped hamster are still unknown.In this study,real-time quantitative polymerase chain reaction was employed to examine the expression profiles of KiSS-1 and GPR54 in the hypothalamus,ovaries,testes,uterus and epididymis of striped hamsters across 4 different seasons.Our results showed that,across different seasons,the KiSS-1 expression mode of male striped hamsters and the GPR54 expression mode of female striped hamsters were consistent with the seasonal photoperiod in the hypothalamus.Meanwhile,across different seasons,the expression profile of KiSS-1 in the testes and the GPR54 expression profile of male striped hamsters in the hypothalamus were consistent with the intensity of their seasonal reproductive activity.Among different tissues,the expression trend for GPR54 is consistent across 4 seasons,while that for KiSS-1 is tissue-dependent.The expression trend for GPR54 across 4 seasons is the same regardless of gender,while that for KiSS-1 is dramatically different and sex-dependent across different seasons.These results suggest that the expressions of KiSS-1 and GPR54 in the striped hamsters were regulated by complicated mechanisms,and the regulatory mechanisms in the striped hamsters are seasonal-dependent and sex-dependent.This research will provide a theoretical basis for studying how KiSS-1 and GPR54 affect seasonal reproduction and the mechanisms behind their influence.
