期刊文献+
共找到7篇文章
< 1 >
每页显示 20 50 100
SPE和QuEChERS净化测定番茄中链格孢霉毒素方法比较 被引量:5
1
作者 周贻兵 兰优 +2 位作者 李磊 吴玉田 郭华 《食品研究与开发》 CAS 北大核心 2019年第22期175-179,196,共6页
通过加标样品的回收率对固相萃取和QuEChERS测定番茄中链格孢霉毒素净化效果进行比较,结果表明,固相萃取净化法的回收率在21%~69%,远低于QuEChERS方法的平均回收率81%~115%,且QuEChERS方法较固相萃取净化法操作简便快速,更适用于番茄中... 通过加标样品的回收率对固相萃取和QuEChERS测定番茄中链格孢霉毒素净化效果进行比较,结果表明,固相萃取净化法的回收率在21%~69%,远低于QuEChERS方法的平均回收率81%~115%,且QuEChERS方法较固相萃取净化法操作简便快速,更适用于番茄中链格孢霉毒素含量的测定。 展开更多
关键词 固相萃取 QUECHERS 番茄 链格孢霉毒素
下载PDF
SARS-CoV-2感染诱导宿主炎性损伤的毒理学机制研究进展
2
作者 车琳 兰尤 +1 位作者 林锦贤 林育纯 《厦门大学学报(自然科学版)》 CAS CSCD 北大核心 2021年第1期1-13,共13页
严重急性呼吸综合征冠状病毒2(SARS-CoV-2)引起的新型冠状病毒肺炎(COVID-19)已成为国际关注的突发公共卫生事件.SARS-CoV-2的传播、致病机制、疫苗研发等成为当前研究焦点,其感染可诱导宿主靶细胞中病原体相关分子模式(PAMPs)和损伤相... 严重急性呼吸综合征冠状病毒2(SARS-CoV-2)引起的新型冠状病毒肺炎(COVID-19)已成为国际关注的突发公共卫生事件.SARS-CoV-2的传播、致病机制、疫苗研发等成为当前研究焦点,其感染可诱导宿主靶细胞中病原体相关分子模式(PAMPs)和损伤相关分子模式(DAMPs)激活、介导细胞器损伤和细胞毒性作用,并通过诱发级联免疫炎症反应介导组织器官的损伤等,该过程的毒理学机制和生物标志亟待阐明.该文从SARS-CoV-2进入宿主、作用于不同靶细胞出发,聚焦于信号通路分子、细胞器、细胞和靶器官等毒性通路的级联作用模式,总结SARS-CoV-2感染诱导毒性作用效应的研究进展,为探索环境生物因素暴露(如冠状病毒感染)诱导机体毒性损伤的靶点筛查、靶向干预及其应用于毒理学安全性评价提供参考. 展开更多
关键词 SARS-Cov-2 毒性作用 毒理学机制 炎性损伤 安全性评价
下载PDF
孢粉证据揭示MIS5a以来福建东北沿海地区人类活动、海平面及气候变化 被引量:15
3
作者 于俊杰 彭博 +6 位作者 兰佑 武彬 王继龙 丁大林 劳金秀 李帅丽 戴璐 《地球科学》 EI CAS CSCD 北大核心 2021年第1期281-292,共12页
通过获取了位于福建省宁德市海岸带的两个钻孔,并使用孢粉分析的方法来重建古环境.光释光和放射性碳十四测年结果表明,钻孔中最老的沉积物来自于深海氧同位素(MIS5a)阶段.孢粉组合中,海相沟鞭藻出现在MIS5a,MIS3早期和早、中全新世阶段... 通过获取了位于福建省宁德市海岸带的两个钻孔,并使用孢粉分析的方法来重建古环境.光释光和放射性碳十四测年结果表明,钻孔中最老的沉积物来自于深海氧同位素(MIS5a)阶段.孢粉组合中,海相沟鞭藻出现在MIS5a,MIS3早期和早、中全新世阶段,反映了这些时段的海侵事件.MIS5a以来,孢粉组合受控于常绿栎(Quercus)和松属(Pinus),指示了周边地区一直被亚热带阔叶林和松林所覆盖,表现了偏暖的亚热带气候.因此,孢粉证据揭示了末次盛冰期时该地区不存在大幅度的降温.芒萁属孢子和草本花粉的剧增指示了晚全新世以来宁德地区人类活动对天然植被的破坏. 展开更多
关键词 福建宁德 孢粉 人类活动 海平面变化 深海氧同位素5a阶段 海洋地质
原文传递
肺炎克雷伯菌外膜囊泡的提取、鉴定及相对定量 被引量:3
4
作者 蓝优 周茂 +4 位作者 陈霞 钟一鸣 李军 晏群 刘文恩 《中华检验医学杂志》 CAS CSCD 北大核心 2022年第2期164-168,共5页
目的基于超速离心改良法快速分离提取肺炎克雷伯菌分泌的外膜囊泡并进行鉴定和定量。方法将购于国家卫生健康委员会临床检验中心经典肺炎克雷伯菌(cKP)标准菌株和台湾大学馈赠高毒力肺炎克雷伯菌(hvKP)标准菌株分别置于M9基础培养基中培... 目的基于超速离心改良法快速分离提取肺炎克雷伯菌分泌的外膜囊泡并进行鉴定和定量。方法将购于国家卫生健康委员会临床检验中心经典肺炎克雷伯菌(cKP)标准菌株和台湾大学馈赠高毒力肺炎克雷伯菌(hvKP)标准菌株分别置于M9基础培养基中培养9 h,利用超速离心改良法提取外膜囊泡(OMV),然后采用负染法于透射电子显微镜下观察其形态和大小,通过Stewart磷脂分析法进行相对定量,并采用两独立样本t检验进行两组间比较。结果透射电子显微镜下观察发现,cKP和hvKP分泌OMV的形态大部分呈球形、囊状小泡结构,小部分形态不规则,OMV直径为20~250 nm,可见多个囊泡聚集成团,且hvKP分泌的OMV多于cKP。相对定量检测发现,hvKP分泌的OMV数量多于cKP,差异有统计学意义(P<0.05)。结论通过超速离心改良法成功实现了肺炎克雷伯菌OMV提取、鉴定及定量检测,为后续OMV机制及功能研究奠定了基础,为抗细菌感染治疗提供了新思路。 展开更多
关键词 克雷伯菌 肺炎 外膜囊泡 超速离心 感染
原文传递
Dorroh extensions of algebras and coalgebras
5
作者 lan you Huixiang CHEN 《Frontiers of Mathematics in China》 SCIE CSCD 2021年第3期857-888,共32页
We study Dorroh extensions of algebras and Dorroh extensions of coalgebras.Their structures are described.Some properties of these extensions are presented.We also introduce the finite duals of algebras and modules wh... We study Dorroh extensions of algebras and Dorroh extensions of coalgebras.Their structures are described.Some properties of these extensions are presented.We also introduce the finite duals of algebras and modules which are not necessarily unital.Using these finite duals,we determine the dual relations between the two kinds of extensions. 展开更多
关键词 Dorroh extension of algebra Dorroh extension of coalgebra Dorroh pair of coalgebras finite dual
原文传递
A location-aware GIServices quality prediction model via collaborative filtering
6
作者 Qingxi Peng lan you Na Dong 《International Journal of Digital Earth》 SCIE EI 2018年第9期897-912,共16页
The quality of GIServices(QoGIS)is an important consideration for services sharing and interoperation.However,QoGIS is a complex concept and difficult to be evaluated reasonably.Most of the current studies have focuse... The quality of GIServices(QoGIS)is an important consideration for services sharing and interoperation.However,QoGIS is a complex concept and difficult to be evaluated reasonably.Most of the current studies have focused on static and non-scalable evaluation methods but have ignored location sensitivity subsequently resulting in the inaccurate QoGIS values.For intensive geodata and computation,GIServices are more sensitive to the location factor than general services.This paper proposes a location-aware GIServices quality prediction model via collaborative filtering(LAGCF).The model uses a mixed CF method based on time zone feature from the perspectives of both user and GIServices.Time zone is taken as the location factor and mapped into the prediction process.A time zone-adjusted Pearson correlation coefficient algorithm was designed to measure the similarity between the GIServices and the target,helping to identify highly similar GIServices.By adopting a coefficient of confidence in the final generation phase,the value of the QoGIS most similar to the target services will play a dominant role in the comprehensive result.Two series of experiments on large-scale QoGIS data were implemented to verify the effectivity of LAGCF.The results showed that LAGCF can improve the accuracy of QoGIS prediction significantly. 展开更多
关键词 LOCATION-AWARE QoGIS quality prediction GIServices collaborative filtering
原文传递
Versioned geoscientific workflow for the collaborative geosimulation of human-nature interactions-a case study of global change and human activities
7
作者 Yuting Chen Hui Lin +5 位作者 Ling Xiao Qi Jing lan you Yulin Ding Mingyuan Hu Adam Thomas Devlin 《International Journal of Digital Earth》 SCIE 2021年第4期510-539,共30页
Global change refers to changes in the relationship between humans and nature.It is desirable to actively integrate human social activities into the unified framework of global change so that their mutual relations an... Global change refers to changes in the relationship between humans and nature.It is desirable to actively integrate human social activities into the unified framework of global change so that their mutual relations and functional mechanisms can be understood.This complicated issue necessitates an appropriate method allowing domain experts to collaboratively contribute their knowledge to geoscientific research.Also,an efficient approach to optimize experimentation is of great importance.The reproducibility of research methods and results needs to be improved to boost the sharing of geographic knowledge and resources.This paper proposes a versioned geoscientific workflow and characterizes its full lifecycle using Virtual Geographic Environments,intending to facilitate and improve research related to the interactions between global change and human activities.The geoscientific workflow management is realized using the concept of version management,making geographic simulation methods and computational results easily reproducible and extendable.The sharing and reuse of geographic knowledge in various forms are archived through version management of geoscientific workflows.A versatile prototype system is implemented which enables the visual modeling of geoscientific workflows,the interactive optimization and collaborative evaluation of geoscientific workflows at runtime,the multi-dimensional dynamic visualization of geo-workflow outputs,and role-based access control for data security. 展开更多
关键词 Geoscientific workflow geographic simulation version management global change virtual geographic environment
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部