Mercury is currently widely used in industries which leads to various means of Hg（II） waste exposure and its accumulation in organisms will cause neurological damages. Thus, there is a great need for the design of p...Mercury is currently widely used in industries which leads to various means of Hg（II） waste exposure and its accumulation in organisms will cause neurological damages. Thus, there is a great need for the design of probes or sensors with high sensitivity and selectivity for detecting and monitoring Hg（II） at physiological pH. Thus a novel and simple molecular probe P1 was prepared from 1,1＇-（1,3-phenylene）-bis（2,4-pentanedionato） for sensing Hg（II） via chelation-enhanced fluorescence （CHEF） mechanism. The probe indicated a selectively fluorescent response to Hg（II） in aqueous media excited by the ultraviolet light of 254 nm. The recognition mechanism was further studied by semi-empirical AM1 and molecular mechanics MM＋ methods in HyperChem 8.0. The calculation indicated a tetrahedron coordination geometry for Hg（II） and a chair-like configuration for the total molecule. The fluorescent images sensing Hg（II） in living mouse fibroblast cells by the probe were obtained by fluorescence microscope.展开更多
文摘Mercury is currently widely used in industries which leads to various means of Hg（II） waste exposure and its accumulation in organisms will cause neurological damages. Thus, there is a great need for the design of probes or sensors with high sensitivity and selectivity for detecting and monitoring Hg（II） at physiological pH. Thus a novel and simple molecular probe P1 was prepared from 1,1＇-（1,3-phenylene）-bis（2,4-pentanedionato） for sensing Hg（II） via chelation-enhanced fluorescence （CHEF） mechanism. The probe indicated a selectively fluorescent response to Hg（II） in aqueous media excited by the ultraviolet light of 254 nm. The recognition mechanism was further studied by semi-empirical AM1 and molecular mechanics MM＋ methods in HyperChem 8.0. The calculation indicated a tetrahedron coordination geometry for Hg（II） and a chair-like configuration for the total molecule. The fluorescent images sensing Hg（II） in living mouse fibroblast cells by the probe were obtained by fluorescence microscope.
