Micropropagation techniques were set up for walnut. Many plantlets were obtained by culturing node explants in MS medium,with 30 g/L sucrose, 7 g/L agar, and different concentrations of BA or Kin alone and different c...Micropropagation techniques were set up for walnut. Many plantlets were obtained by culturing node explants in MS medium,with 30 g/L sucrose, 7 g/L agar, and different concentrations of BA or Kin alone and different concentrations of BA ＋ NAA or BA ＋ Kin. BA at concentration （2 mg L-1 BA） gave the best results in percentage response and number of shoots/plant （100%, 2.84） respectively. While, Kin at 3 mg L-1 gave the highest average shoot length （2.81 cm）. However, MS medium supplemented with 2.5 mg L-1 ＋ 0.5 mg L-1 Kin gave the best results compared with all treatment shoot/explants and the highest number of leaves/explants （3.7, 8.2） respectively. While the highest length of shoot （4.7 cm） in MS medium supplemented with 3 mg L-1 Kin. and a high rooting percentage up to 60% at MS medium supplemented with 1 mg L-1 IBA （Indole-3-butyric acid） was obtained.展开更多
文摘Micropropagation techniques were set up for walnut. Many plantlets were obtained by culturing node explants in MS medium,with 30 g/L sucrose, 7 g/L agar, and different concentrations of BA or Kin alone and different concentrations of BA ＋ NAA or BA ＋ Kin. BA at concentration （2 mg L-1 BA） gave the best results in percentage response and number of shoots/plant （100%, 2.84） respectively. While, Kin at 3 mg L-1 gave the highest average shoot length （2.81 cm）. However, MS medium supplemented with 2.5 mg L-1 ＋ 0.5 mg L-1 Kin gave the best results compared with all treatment shoot/explants and the highest number of leaves/explants （3.7, 8.2） respectively. While the highest length of shoot （4.7 cm） in MS medium supplemented with 3 mg L-1 Kin. and a high rooting percentage up to 60% at MS medium supplemented with 1 mg L-1 IBA （Indole-3-butyric acid） was obtained.
