Evaluation of Reverse Transcription Loop-Mediated Isothermal Amplification assays for Rapid Detection of Human Enterovirus 71 and Coxsackievirus A16 in Clinical Samples

A sensitive reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for human enterovirus 71 (EV71) and Coxsackievirus A16 (CVA16) infection was further evaluated. The one step reaction was performed in a single tube at 65?C for 45 min for EV71 and 35 min for CVA16. The detection limits of RT-LAMP assays for both EV71 and CVA16 were 0.1 of a 50% tissue culture infective dose (TCID50) per reaction, based on 10—Fold dilutions of a titrated EV71 or CVA16 strain. The specific assay showed there were no cross-reactions with Coxsackievirus A (CVA) viruses (CVA 2, 4, 5, 7, 9, 10, 14, and 25), Coxsackievirus B (CVB) viruses (CVB 1, 2, 3, 4, and 5) or ECHO viruses (ECHO 3, 6, 11, and 19). In parallel with commercial quantitative real-time polymerase chain reaction (qRT-PCR) diagnostic kits for EV71 and CVA16, the RT-LAMP assay was evaluated with 515 clinical specimens, the results showed the RT-LAMP assay and the qRT-PCR assay were in complete agreement for 513/515 (99.6%) of the specimens. Two samples with discrepant results from two methods were further verified by nested reverse transcription polymerase chain reaction (nRT-PCR) assay and sequencing to be true positives for CVA16. In conclusion, RT-LAMP assay is demonstrated to be a sensitive and specific assay and have a great potential for the rapid and visual screening of EV71 and CVA16 in China, especially in those resource-limited hospitals and rural clinics of provincial and municipal regions.

抗病毒治疗对乙型肝炎相关性肝癌术后肝内复发患者生存状况的影响

目的探讨抗病毒治疗对乙型肝炎病毒(HBV)相关肝癌术后肝内复发患者生存状况的影响。方法回顾性分析2012年1月-2013年6月四川省人民医院107例乙型肝炎相关性肝癌术后发生肝内复发患者的临床资料,根据患者复发后抗病毒治疗情况分为抗病毒组(68例)和对照组(39例)。比较两组患者复发后累积生存时间、复发后6个月乙型肝炎-DNA(HBV-DNA)、乙型肝炎病毒e抗原(HBe-Ag)及甲胎蛋白(AFP)水平。采用Kaplan-Meier法统计两组患者的2年生存率,Cox回归模型分析与患者复发后2年生存率相关的因素。结果抗病毒组的中位生存时间为24个月(15.6~26.1个月),对照组的中位生存时间为14个月(11.2~20.3个月),差异均有统计学意义(P<0.05)。抗病毒组1和2年累积生存率分别为79.71%和39.12%,对照组1和2年累积生存率分别为58.41%和15.71%,差异有统计学意义(P<0.05)。经过正规抗病毒治疗6个月后,抗病毒组患者的HBV-DNA拷贝数低于对照组(P<0.05)。Cox多因素分析显示:原发肝癌的组学分化程度、复发肿瘤≥2 cm及抗病毒治疗是影响肝癌患者肝内复发后2年生存率的主要因素。结论对存在抗病毒治疗指征的肝癌术后肝内复发患者采用正规的抗病毒治疗对提高患者的生存率有积极的作用。

Validation of the chinese version of the EORTC QLQ-CR29 in patients with colorectal cancer

AIM To assess the validity and reliability of the European Organization for Research and Treatment of Cancer Quality of Life Questionnaire-Colorectal Cancer 29(EORTC QLQ-CR29) in Chinese patients with colorectal cancer(CRC). METHODS From March 2014 to January 2015, 356 patients with CRC from four different hospitals in China were enrolled in the study, and all patients self-administered the EORTC QLQ-CR29 and the quality of life core questionnaire(EORTC QLQ-C30). Evaluation of the scores was based on the Karnofsky Performance Scale(KPS). The reliability and validity of the questionnaires were assessed by Cronbach's α coefficient, the Spearman correlation test and Wilcoxon rank sum test.RESULTS The EORTC QLQ-CR29 showed satisfactory reliability(α > 0.7), although the urinary frequency and blood and mucus in stool dimensions had only moderate reliability(α = 0.608). The multitrait scaling analyses showed good convergent(r > 0.4) and discriminant validity. Significant differences were obtained for each item in the different KPS subgroups(KPS ≤ 80; KPS > 80). body image and most single-item dimensions showed statistically significant differences in patients with a stoma compared with the rest of the patients. CONCLUSION The EORTC QLQ-CR29 exhibits high validity and reliability in Chinese patients with CRC, and can therefore be recommended as a valuable tool for the assessment of quality of life in these patients.

Clinical Outcome of Autologous Hematopoietic Stem Cell Infusion via Hepatic Artery or Portal Vein in Patients with End-stage Liver Diseases

Objective To investigate the efficacy of hematopoietic stem cell(HSC) transplantation via the hepatic artery vs.the portal vein for end-stage liver disease(ESLD).Methods Patients with hepatic decompensation were prospectively recruited from September 2010 to September 2012 to receive HSC transplantation via the hepatic artery or the portal vein.Liver function was examined at 3,6,and 12 months after transplantation.Liver biopsy results were analyzed using the Knodell score.Results Eighty patients(58 males and 22 females) were enrolled in the study.The Child-Pugh score was grade B in 69 cases,and grade C in the remaining 11 cases.HSC transplantation was performed via the portal vein in 36 patients and via the hepatic artery in 44 patients.ALT levels decreased while serum albumin levels increased significantly in both groups at 6 and 12 months after HSC transplantation(P<0.05 compared with pre-transplantation levels).Total bilirubin levels decreased significantly in both groups at 3,6,and 12 months after HSC transplantation(P<0.05 compared with pre-transplantation levels).Additionally,prothrombin time decreased in both groups at 12 months after HSC transplantation(P<0.05 compared with pre-transplantation level).There were no significant differences in ALT,total bilirubin and prothrombin time between the two groups either before or after transplantation.Moreover,Knodell score decreased significantly at 6 and 12 months.Histological examination showed that liver cell edema,degeneration,necrosis,and inflammation were significantly relieved at 3,6,and 12 months after transplantation.The incidence of portal vein thrombosis,upper gastrointestinal bleeding,and hepatic encephalopathy were 1.25%,3.75%,and 2.5% respectively.The one-year survival rate was 100%.Conclusions Autologous HSC transplantation improves liver function and histology in ESLD patients.The administration route of HSC has no significant impact on the efficacy of transplantation.

Expansion of PmBEAT genes in the Prunus mume genome induces characteristic floral scent production

Prunus mume is the only plant in the genus Prunus of the Rosaceae family with a characteristic floral scent,and the main component of this scent is benzyl acetate.By contrast,benzyl acetate is not synthesized in Prunus persica flowers.Here,we searched for benzyl alcohol acetyltransferase(BEAT)genes based on genomic data from P.mume and P.persica and found 44 unique PmBEATs in P.mume.These genes,which were mainly detected in clusters on chromosomes,originated from gene duplication events during the species evolution of P.mume,and retroduplication and tandem duplication were the two dominant duplication patterns.The genes PmBEAT34,PmBEAT36 and PmBEAT37,which were generated by tandem duplication,were highly expressed in flowers,and their highest levels were detected during the blooming stage.In vitro,PmBEAT34,PmBEAT3,and PmBEAT37 all had benzyl alcohol acetyltransferase activity that was localized in the cytoplasm.Overexpression of the PmBEAT36 or PmBEAT37 genes increased benzyl acetate production in the petal protoplasts of P.mume,and interference in the expression of these genes slightly decreased the benzyl acetate content.In addition,light and temperature regulated the expression of the PmBEAT34,PmBEAT36 and PmBEAT37 genes.According to these results,we hypothesize that the expansion of the PmBEAT genes in the genome induce the characteristic floral scent of P.mume.

离心风机叶轮气动性能及噪声优化研究

基于CFD数值仿真优化设计方法,以某型高效离心通风机叶轮为研究对象,从气体流动机理出发,对前盘结构进行优化设计,旨在提高风机气动性能,降低风机气动噪声。结果表明,对该型离心通风机,通对对叶轮前盘优化,效率增加1.7%,全压增加2.1%,A计权声压级降低1.5dB(A)。通过样机制造及试验测试,优化后的机型全压效率为79.5%,A计权声压级为73.3dB(A),比A声级为7.6dB(A)。

The effect and mechanism of Tip60 regulating DNA on embryo development

Objective: To study the effect and mechanism of Tip60 regulating DNA on mouse embryo development, and to provide theoretical basis for the study of embryo development. Methods: mice embryos were randomly divided into two groups: control group and experimental group. The embryos of experimental group were given Tip60 inhibitor, the mice of control group were given the same dose of normal saline. The level of Tip60 in the two groups of mice embryos, the effect of Tip60 on p53-p21 pathway, the expression of DNA repair factor 53BP1 protein, the content of active oxygen, the effect of autophagy and apoptosis, and the development of embryos were analyzed. Results: the level of Tip60 in the experimental group was significantly lower than that in the control group;the p53-p21 pathway in the experimental group was activated, the DNA damage of the experimental group was greater, the expression of DNA repair factor was lower, the content of ROS in the experimental group was significantly higher than that in the control group;the autophagy and apoptosis of the experimental group were enhanced;the capsule of the control group was enhanced. The embryogenesis rate of the experimental group was (65.13 ± 4.85)%, and that of the experimental group was (29.36 ± 1.75)%, which was significantly lower than that of the control group. Conclusion: Tip60 can regulate DNA damage and repair by mediating p53-p21 pathway in early mouse embryo development. Inhibition of Tip60 can increase the active oxygen, induce autophagy and over expression of apoptosis in mouse embryo cells, and inhibit the development of embryo to a certain extent. It has important guiding significance to measure the level of Tip60 during embryo culture.

Optimal Control Policy of M/G/1 Queueing System with Delayed Randomized Multiple Vacations Under the Modified Min(N,D)-Policy Control

Based on the number of customers and the server’s workload,this paper proposes a modified Min(N,D)-policy and discusses an M/G/1 queueing model with delayed randomized multiple vacations under such a policy.Applying the well-known stochastic decomposition property of the steady-state queue size,the probability generating function of the steady-state queue length distribution is obtained.Moreover,the explicit expressions of the expected queue length and the additional queue length distribution are derived by some algebraic manipulations.Finally,employing the renewal reward theorem,the explicit expression of the long-run expected cost per unit time is given.Furthermore,we analyze the optimal policy for economizing the expected cost and compare the optimal Min(N,D)-policy with the optimal N-policy and the optimal D-policy by using numerical examples.

Molecular collisions:From near-cold to ultra-cold

In the past two decades,the revolutionary technologies of creating cold and ultracold molecules have provided cutting-edge experiments for studying the fundamental phenomena of collision physics.To a large degree,the recent explosion of interest in the molecular collisions has been sparked by dramatic progress of experimental capabilities and theoretical methods,which permit molecular collisions to be explored deep in the quantum mechanical limit.Tremendous experimental advances in the field have already been achieved,and the authors,from an experimental perspective,provide a review of these studies for exploring the nature of molecular collisions occurring at temperatures ranging from the Kelvin to the nanoKelvin regime,as well as for applications of producing ultracold molecules.

Simultaneous Zeeman deceleration of polyatomic free radical with lithium atoms

Chemistry in the ultracold regime enables fully quantum-controlled interactions between atoms and molecules,leading to the discovery of the hidden mechanisms in chemical reactions which are usually curtained by thermal averaging in the high temperature.Recently a couple of diatomic molecules have been cooled to ultracold regime based on laser cooling techniques,but the chemistry associated with these simple molecules is highly limited.In comparison,free radicals play a major role in many important chemical reactions,but yet to be cooled to submillikelvin temperature.Here we propose a novel method of decelerating CH_(3),the simplest polyatomic free radical,with lithium atoms simultaneously by travelling wave magnetic decelerator.This scheme paves the way towards co-trapping CH_(3)and lithium,so that sympathetical cooling can be used to preparing ultracold free radical sample.

Distributed exact Grover’s algorithm

Distributed quantum computation has gained extensive attention.In this paper,we consider a search problem that includes only one target item in the unordered database.After that,we propose a distributed exact Grover’s algorithm(DEGA),which decomposes the original search problem into■n/2■parts.Specifically,(i)our algorithm is as exact as the modified version of Grover’s algorithm by Long,which means the theoretical probability of finding the objective state is 100%;(ii)the actual depth of our circuit is 8(n mod 2)+9,which is less than the circuit depths of the original and modified Grover’s algorithms,1+8■π/4√2^(n)■and 9+8■π/4√2^(n)-1/2■,respectively.It only depends on the parity of n,and it is not deepened as n increases;(iii)we provide particular situations of the DEGA on MindQuantum(a quantum software)to demonstrate the practicality and validity of our method.Since our circuit is shallower,it will be more resistant to the depolarization channel noise.

Quantum simulation of a general anti-PT-symmetric Hamiltonian with a trapped ion qubit

Non-Hermitian systems satisfying parity-time(PT)symmetry have aroused considerable interest owing to their exotic features.Anti-PT symmetry is an important counterpart of the symmetry,and has been studied in various classical systems.Although a Hamiltonian with anti-PT symmetry only differs from its PT-symmetric counterpart in a global phase,the information and energy exchange between systems and environment are different under them.It is also suggested theoretically that anti-PT symmetry is a useful concept in the context of quantum information storage with qubits coupled to a bosonic bath.So far,the observation of anti-PT symmetry in individual quantum systems remains elusive.Here,we implement an anti-PT-symmetric Hamiltonian of a single qubit in a single trapped ion by a designed microwave and optical control-pulse sequence.We characterize the anti-PT phase transition by mapping out the eigenvalues at different ratios between coupling strengths and dissipation rates.The full information of the quantum state is also obtained by quantum state tomography.Our work allows quantum simulation of genuine open-system feature of an anti-PT-symmetric system,which paves the way for utilizing non-Hermitian properties for quantum information processing.

Biodegradation of 3,5-dimethyl-2,4-dichlorophenol in saline wastewater by newly isolated Penicillium sp.yz11-22N2

In this study,the performance of 3,5-dimethyl-2,4-dichlorophenol(DCMX) degradation by a screened strain was investigated.18 S r DNA and the neighbor-joining method were used for identification of the isolated strain.The results of phylogenetic analysis and scanning electron micrographs showed that the most probable identity of the screened strain should be Penicillium sp.Growth characteristics of Penicillium sp.and degradation processes of DCMX were examined.Fourier transform infrared spectroscopy of the inoculated DCMX solution was recorded,which supported the capacity of DCMX degradation by the screened Penicillium sp.Under different salinity conditions,the highest growth rate and removal efficiency for DCMX were obtained at p H 6.0.The removal efficiency decreased from 100%to 66% when the DCMX concentration increased from 5 to 60 mg/L,respectively.Using a Box–Behnken design,the maximum DCMX removal efficiency was determined to be 98.4%.With acclimation to salinity,higher removal efficiency could be achieved.The results demonstrate that the screened Penicillium sp.has the capability for degradation of DCMX.