312 recombinant inbred lines （RILs） in F9 from a cross between a overwintering cold-tolerant germplasm resource Glutinous rice 89-1（Gr 89-1） and a cold-sensitive variety Shuhui 527 was used for quantitative trait ...312 recombinant inbred lines （RILs） in F9 from a cross between a overwintering cold-tolerant germplasm resource Glutinous rice 89-1（Gr 89-1） and a cold-sensitive variety Shuhui 527 was used for quantitative trait locus （QTL） analysis. The scores of percent ratooning germinability （PRG） and overwintering germinability （POG） were evaluated. The overwintering germination rate of axillary buds was scored to represent the overwintering germinability. Two significant QTLs （qPRG-4 and qPRG-7） on chromosomes 4 and 7 were detected and explained 8.3 and 7.2% of the total phenotypic variation, respectively. Three significant QTLs （qPOG-2, qPOG-3 and qPOG-7） were identified and mapped on chromosomes 2, 3, and 7, respectively. These QTLs contributed 9.6, 6.7, and 17.8% of phenotypic variations, respectively. A comparative analysis using SSR markers closely linked to the three QTLs for the overwintering revealed cold-tolerant individuals, which harbour the Glutinous rice 89-1 alleles at RM7110, RM250, RM418, and RM232, had a high percent overwintering germinability, while cold-sensitive individuals, which carry Shuhui 527 alleles at these loci, had a low percent overwintering germinability in the F2 population of Shuhui 527/Glutinous rice 89-1. This study demonstrated the utility of these SSR markers for selection of overwintering germinability genotypes.展开更多
Microbial bioelectrochemical system(BES)is a promising sustainable technology for the electrical energy recovery and the treatment of recalcitrant and toxic pollutants.In microbial BESs,the conversion of harmful pollu...Microbial bioelectrochemical system(BES)is a promising sustainable technology for the electrical energy recovery and the treatment of recalcitrant and toxic pollutants.In microbial BESs,the conversion of harmful pollutants into harmless products can be catalyzed by microorganisms at the anode(Type I BES),chemical catalysts at the cathode(Type II BES)or microorganisms at the cathode(Type III BES).The application of synthetic biology in microbial BES can improve its pollutant removing capability.Synthetic biology techniques can promote EET kinetics,which is helpful for microbial anodic electro-respiration,expediting pollutant removing not only at the anode but also at the cathode.They offer tools to promote biofilm development on the electrode,enabling more microorganisms residing on the electrode for subsequent catalytic reactions,and to overexpress the pollutant removing-related genes directly in microorganisms,contributing to the pollutant decomposition.In this work,based on the summarized aspects mentioned above,we describe the major synthetic biology strategies in designing and improving the pollutant removing capabilities of microbial BES.Lastly,we discuss challenges and perspectives for future studies in the area.展开更多
The Hexi Corridor in northwest China has for millennia served as a crossroads for interactions between East Asia,Central Asia and lands further west,while also connecting the Chinese heartland and the northern Mongoli...The Hexi Corridor in northwest China has for millennia served as a crossroads for interactions between East Asia,Central Asia and lands further west,while also connecting the Chinese heartland and the northern Mongolian Steppe and Tibetan Plateau(TP)to the south(Fig.1a).Previous studies have revealed the details of this cross-continental material and cultural transmission as early as the Bronze Age onwards.Materials and technologies exchanged along the ancient Silk Road included East Asian millets and painted pottery,West Asian wheat and barley,bronze metal-lurgy,domesticated sheep,horses,and cattle[1].To date.展开更多
To quantitatively determine tylosin and tilmicosin in edible animal tissues,a time-resolved fluoroimmunoassay(TRFIA) has been developed and validated.For this purpose,desmycosin-O-carboxymethoxylamine-BSA was fixed on...To quantitatively determine tylosin and tilmicosin in edible animal tissues,a time-resolved fluoroimmunoassay(TRFIA) has been developed and validated.For this purpose,desmycosin-O-carboxymethoxylamine-BSA was fixed onto microtiter plates,standards and samples were loaded and,finally,diluted europium-labeled anti-tylosin antibodies were added.Results show that the limit of detection for tylosin was 0.03 ng mL-1 and that for tilmicosin was 0.05 ng mL-1.The recoveries were 73.6% to 120.5%,with coefficients of variation below 15.6% in various biological matrices spiked with tylosin and tilmicosin at concentrations of 50-200 ngg-1.There was good correlation(R2>0.99) between the TRFIA,an enzyme-linked immunosorbent assay and high performance liquid chromatography data.In conclusion,the new TRFIA is applicable to the detection of tylosin and tilmicosin and is an effective and economical method that will enable high-throughput sample screening.The method is expected to be widely applicable.展开更多
Microplastics are ubiquitous in the global environment.As a typical emerging pollutant,its potential health hazards have been widely concerning.In this brief paper,we introduce the source,identification,toxicity,and h...Microplastics are ubiquitous in the global environment.As a typical emerging pollutant,its potential health hazards have been widely concerning.In this brief paper,we introduce the source,identification,toxicity,and health hazard of microplastics in the human.The literature review shows that microplastics are frequently detected in environmental and human samples.Humans are potentially exposed to microplastics through oral intake,inhalation,and skin contact.We summarize the toxic effects of microplastics in experimental models like cells,organoids,and animals.These effects consist of oxidative stress,DNA damage,organ dysfunction,metabolic disorder,immune response,neurotoxicity,as well as reproductive and developmental toxicity.In addition,the epidemiological evidence suggests that a variety of chronic diseases may be related to microplastics exposure.Finally,we put forward the gaps in toxicity research of microplastics and their future development directions.This review will be helpful to the understanding of the exposure risk and potential health hazards of microplastics.展开更多
基金supported by the Rice Key Project of Chongqing,China (CSTC2011AB1076)the Foundation Project of Chongqing Education Commission (KJ110623)+1 种基金the Doctor Foundation of Chongqing Normal University,China (10LR028)the Plants Breeding Project of Chongqing,China (CSTC2010AA1033)
文摘312 recombinant inbred lines （RILs） in F9 from a cross between a overwintering cold-tolerant germplasm resource Glutinous rice 89-1（Gr 89-1） and a cold-sensitive variety Shuhui 527 was used for quantitative trait locus （QTL） analysis. The scores of percent ratooning germinability （PRG） and overwintering germinability （POG） were evaluated. The overwintering germination rate of axillary buds was scored to represent the overwintering germinability. Two significant QTLs （qPRG-4 and qPRG-7） on chromosomes 4 and 7 were detected and explained 8.3 and 7.2% of the total phenotypic variation, respectively. Three significant QTLs （qPOG-2, qPOG-3 and qPOG-7） were identified and mapped on chromosomes 2, 3, and 7, respectively. These QTLs contributed 9.6, 6.7, and 17.8% of phenotypic variations, respectively. A comparative analysis using SSR markers closely linked to the three QTLs for the overwintering revealed cold-tolerant individuals, which harbour the Glutinous rice 89-1 alleles at RM7110, RM250, RM418, and RM232, had a high percent overwintering germinability, while cold-sensitive individuals, which carry Shuhui 527 alleles at these loci, had a low percent overwintering germinability in the F2 population of Shuhui 527/Glutinous rice 89-1. This study demonstrated the utility of these SSR markers for selection of overwintering germinability genotypes.
基金the National Key Research and Development Program of China(2021YFA0910300).
文摘Microbial bioelectrochemical system(BES)is a promising sustainable technology for the electrical energy recovery and the treatment of recalcitrant and toxic pollutants.In microbial BESs,the conversion of harmful pollutants into harmless products can be catalyzed by microorganisms at the anode(Type I BES),chemical catalysts at the cathode(Type II BES)or microorganisms at the cathode(Type III BES).The application of synthetic biology in microbial BES can improve its pollutant removing capability.Synthetic biology techniques can promote EET kinetics,which is helpful for microbial anodic electro-respiration,expediting pollutant removing not only at the anode but also at the cathode.They offer tools to promote biofilm development on the electrode,enabling more microorganisms residing on the electrode for subsequent catalytic reactions,and to overexpress the pollutant removing-related genes directly in microorganisms,contributing to the pollutant decomposition.In this work,based on the summarized aspects mentioned above,we describe the major synthetic biology strategies in designing and improving the pollutant removing capabilities of microbial BES.Lastly,we discuss challenges and perspectives for future studies in the area.
基金supported by the National Key Research and Development Program(2020YFE0201600)the National Natural Science Foundation of China(32070576,32271186,and 32270667)+14 种基金the National Social Science Fund of China(19VJX074)the Scientific and Technology Committee of Shanghai Municipality(18490750300)Shanghai Municipal Science and Technology Major Project(2017SHZDZX01)the Major Project of National Social Science Foundation of China granted to ChuanChao Wang(21&ZD285)Sheng Han(20&ZD212)the Natural Science Foundation of Fujian Province of China(2023J06013)Major Special Project of Philosophy and Social Sciences Research of the Ministry of Education(2022JZDZ023)the Major Project of National Social Science Foundation of China(2021MZD014)the"Double First Class University Plan"Key Construction Project of Xiamen University(0310/X2106027)Nanqiang Outstanding Young Talents Program of Xiamen University(X2123302)European Research Council(ERC)grant(ERC-2019-ADG-883700-TRAM)China Postdoctoral Science Foundation(KLH3149007)the Natural Science Foundation of Fujian Province of China(2023J06013)Open Research Fund of State Key Laboratory ofGenetic Engineering at Fudan University(SKLGE-2310)Research and Propagation Fund for History and Culture of Gansu Province(2023ZD007)。
文摘The Hexi Corridor in northwest China has for millennia served as a crossroads for interactions between East Asia,Central Asia and lands further west,while also connecting the Chinese heartland and the northern Mongolian Steppe and Tibetan Plateau(TP)to the south(Fig.1a).Previous studies have revealed the details of this cross-continental material and cultural transmission as early as the Bronze Age onwards.Materials and technologies exchanged along the ancient Silk Road included East Asian millets and painted pottery,West Asian wheat and barley,bronze metal-lurgy,domesticated sheep,horses,and cattle[1].To date.
基金supported by the Scientific and Technological Research Project of Chongqing (CSTC2011ggB10009,cstc2012pt-kyys10002,cstc2012cx-rkxB10002)the National Natural Science Foundation of China(81202438)the Natural Science Foundation of the Chongqing Education Committee (KJ110605)
文摘To quantitatively determine tylosin and tilmicosin in edible animal tissues,a time-resolved fluoroimmunoassay(TRFIA) has been developed and validated.For this purpose,desmycosin-O-carboxymethoxylamine-BSA was fixed onto microtiter plates,standards and samples were loaded and,finally,diluted europium-labeled anti-tylosin antibodies were added.Results show that the limit of detection for tylosin was 0.03 ng mL-1 and that for tilmicosin was 0.05 ng mL-1.The recoveries were 73.6% to 120.5%,with coefficients of variation below 15.6% in various biological matrices spiked with tylosin and tilmicosin at concentrations of 50-200 ngg-1.There was good correlation(R2>0.99) between the TRFIA,an enzyme-linked immunosorbent assay and high performance liquid chromatography data.In conclusion,the new TRFIA is applicable to the detection of tylosin and tilmicosin and is an effective and economical method that will enable high-throughput sample screening.The method is expected to be widely applicable.
基金supported by the National Natural Science Foundation of China(Grant nos.22241604 and 22125606).
文摘Microplastics are ubiquitous in the global environment.As a typical emerging pollutant,its potential health hazards have been widely concerning.In this brief paper,we introduce the source,identification,toxicity,and health hazard of microplastics in the human.The literature review shows that microplastics are frequently detected in environmental and human samples.Humans are potentially exposed to microplastics through oral intake,inhalation,and skin contact.We summarize the toxic effects of microplastics in experimental models like cells,organoids,and animals.These effects consist of oxidative stress,DNA damage,organ dysfunction,metabolic disorder,immune response,neurotoxicity,as well as reproductive and developmental toxicity.In addition,the epidemiological evidence suggests that a variety of chronic diseases may be related to microplastics exposure.Finally,we put forward the gaps in toxicity research of microplastics and their future development directions.This review will be helpful to the understanding of the exposure risk and potential health hazards of microplastics.
