Objective:To make full usage of resource and turn waste into treasure,the chemical constituents and bioactivity were firstly investigated on Damask rose(Rosa damascena)flower residue(DRFR).Methods:DPPH and ABTS experi...Objective:To make full usage of resource and turn waste into treasure,the chemical constituents and bioactivity were firstly investigated on Damask rose(Rosa damascena)flower residue(DRFR).Methods:DPPH and ABTS experiments were applied to assess the antioxidant activity of DRFR.Then,column chromatography was used to purify compounds from an antioxidation extract(DRFR-A),and the chemical structure was identified using NMR.The total phenolic acid content was measured by FolinCiocalteu colorimetric method,and the content of gallic acid of the indicator ingredient was detected by HPLC.Results:DRFR-A was found to show a high activity both on DPPH(IC5o:2.760μg/mL)and ABTS(ICso:2.258μg/mL)compared to positive control Vc,Ten compounds were isolated and identified as quercetin(1),kaempferol(2),gallic acid(3),protocatechuic acid(4),pyrogallic acid(5),2-phenylethyl 3,4,5-trihydroxybenzoate(6),methyl gallate(7),p-hydroxybenzoic acid(8),p-hydroxyphenethyl alcohol(9)and astragalin(10)from DRFR-A.Among them,pyrogallic acid,2-phenylethyl-3,4,5-trihydroxybenzoate,p-hydroxybenzoic acid and p-hydroxyphenethyl alcohol are obtained from the plant for the first time.The content of total phenolic acids and gallic acid,main ingredient in DRFR-A was determined as 63.73%and 24.67%,respectively.Conclusion:This study provides a reliable data and lays the foundation for the development and utilization of rose residue,and hence for the full utilization of rose resources.展开更多
Porous hybrid microspheres were fabricated by the synthesized calcium gluconate-g-poly（D,L-lactide） （CG-g- PDLLA） composites. These hybrid microspheres were treated with an alkaline solution for different period o...Porous hybrid microspheres were fabricated by the synthesized calcium gluconate-g-poly（D,L-lactide） （CG-g- PDLLA） composites. These hybrid microspheres were treated with an alkaline solution for different period of time to control the amount of generated carboxylate groups and remained CG on the surface. The microspheres were then incubated in a supersaturated simulated body fluid （1.5 SBF） solution for different time to investigate their biomimetic mineralization behavior. The depositions were found to have a fine cluster morphology, a similar crystal structure and chemical structure to natural hydroxyapatite, and a medium Ca/P of approximately 1.30. The effect of surface treating time on the structure and mineralization behavior of these microspheres has been discussed in detail. The results indicate that the nucleation and growth of apatite on the surface are influenced by the induced carboxylate groups and the remained CG. The hybrid CG-g- PDLLA microspheres have the potential as a novel alternative in bone tissue engineering.展开更多
基金The authors wish to thank associate professor Cheng-xue Pan from Zheng-zhou University for his assistance in the plants identification and authentications.This project was supported financially by the National Natural Science Foundation of China(Grant No.331/313400531021).
文摘Objective:To make full usage of resource and turn waste into treasure,the chemical constituents and bioactivity were firstly investigated on Damask rose(Rosa damascena)flower residue(DRFR).Methods:DPPH and ABTS experiments were applied to assess the antioxidant activity of DRFR.Then,column chromatography was used to purify compounds from an antioxidation extract(DRFR-A),and the chemical structure was identified using NMR.The total phenolic acid content was measured by FolinCiocalteu colorimetric method,and the content of gallic acid of the indicator ingredient was detected by HPLC.Results:DRFR-A was found to show a high activity both on DPPH(IC5o:2.760μg/mL)and ABTS(ICso:2.258μg/mL)compared to positive control Vc,Ten compounds were isolated and identified as quercetin(1),kaempferol(2),gallic acid(3),protocatechuic acid(4),pyrogallic acid(5),2-phenylethyl 3,4,5-trihydroxybenzoate(6),methyl gallate(7),p-hydroxybenzoic acid(8),p-hydroxyphenethyl alcohol(9)and astragalin(10)from DRFR-A.Among them,pyrogallic acid,2-phenylethyl-3,4,5-trihydroxybenzoate,p-hydroxybenzoic acid and p-hydroxyphenethyl alcohol are obtained from the plant for the first time.The content of total phenolic acids and gallic acid,main ingredient in DRFR-A was determined as 63.73%and 24.67%,respectively.Conclusion:This study provides a reliable data and lays the foundation for the development and utilization of rose residue,and hence for the full utilization of rose resources.
基金supported by the National Natural Science Foundation of China(Nos.51025314 and 21104089)the Fundamental Research Funds for the Central Universities(No.ZY1632)
文摘Porous hybrid microspheres were fabricated by the synthesized calcium gluconate-g-poly（D,L-lactide） （CG-g- PDLLA） composites. These hybrid microspheres were treated with an alkaline solution for different period of time to control the amount of generated carboxylate groups and remained CG on the surface. The microspheres were then incubated in a supersaturated simulated body fluid （1.5 SBF） solution for different time to investigate their biomimetic mineralization behavior. The depositions were found to have a fine cluster morphology, a similar crystal structure and chemical structure to natural hydroxyapatite, and a medium Ca/P of approximately 1.30. The effect of surface treating time on the structure and mineralization behavior of these microspheres has been discussed in detail. The results indicate that the nucleation and growth of apatite on the surface are influenced by the induced carboxylate groups and the remained CG. The hybrid CG-g- PDLLA microspheres have the potential as a novel alternative in bone tissue engineering.
