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Optimal cytokinin/auxin balance for indirect shoot organogenesis of Eucalyptus cloeziana and production of ex vitro rooted micro-cuttings 被引量:1
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作者 leandro silva de oliveira Gilvano Ebling Brondani +4 位作者 Letícia Vaz Molinari Rafaella Zanetti Dias Gustavo Leal Teixeira Antônio Natal Gonçalves Marcílio de Almeida 《Journal of Forestry Research》 SCIE CAS CSCD 2022年第5期1573-1584,共12页
Shoot organogenesis is critical for the shortening of long breeding cycles and circumvent the barrier of cloning mature Eucalyptus cloeziana trees.It enables large-scale production of plants from transformed tissues.T... Shoot organogenesis is critical for the shortening of long breeding cycles and circumvent the barrier of cloning mature Eucalyptus cloeziana trees.It enables large-scale production of plants from transformed tissues.This study evaluates the effect ofα-naphthaleneacetic acid(NAA),thidiazuron(TDZ)and benzylaminopurine(BAP)on the organogenesis of E.cloeziana from hypocotyls and cotyledonary leaves.In the induction stage,hypocotyls and cotyledonary leaves were established in a Murashige and Skoog(MS)culture medium supplemented with NAA or TDZ.Callus tissues were cultivated in a MS culture medium containing only BAP or different concentrations of BAP/NAA in the differentiation stage.Adventitious buds were multiplied in vitro and elongated in a WPM culture medium supplemented with 0.89μM BAP and 0.05μM NAA.Cotyledonary leaves exhibited the best in vitro regeneration.The induction of adventitious buds occurred only in calluses induced from TDZ.In the differentiation stage,4.4μM BAP treatment promoted an increase of adventitious bud regeneration.Micro-cuttings from regenerated shoots were acclimatized and rooted ex vitro in mini-incubators.The results confirm the establishment of an efficient protocol for the in vitro regeneration of E.cloeziana by indirect organogenesis,providing new insights regarding cloning of this species. 展开更多
关键词 MICROPROPAGATION Recalcitrant species Plant growth regulator Explants source
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Different culture conditions applied to in vitro shoot multiplication of two Eucalyptus benthamii explant sources
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作者 Natalia Pimentel Esposito-Polesi leandro silva de oliveira +2 位作者 Francisco Jose Benedini Baccarin Cristina Vieira de Almeida Marcilio de Almeida 《Journal of Forestry Research》 SCIE CAS CSCD 2020年第3期857-869,共13页
Eucalyptus adult material requires more successive subcultures in the in vitro multiplication phase for increased vigor and cellular activity. This study evaluated the endophytic manifestation and shoot multiplication... Eucalyptus adult material requires more successive subcultures in the in vitro multiplication phase for increased vigor and cellular activity. This study evaluated the endophytic manifestation and shoot multiplication of one 13-year-old Eucalyptus benthamii clone under different culture conditions and used canopy branches(CB) and trunk base material as explant sources. The culture media were wood plant medium(WPM), Murashige and Skoog medium(MS) and JADS(Correia and co-authors medium).Based on the results of the initial multiplication experiment, further tests examined sucrose concentrations and p H. Morphophysiology, dry mass production, endophyticmanifestation and histochemical were determined. Explant sources responded differently to MS and JADS media, but the WPM medium promoted homogeneous development.The responses were similar for both explant sources when sucrose concentrations varied. Shoots died in the absence of sucrose, showed high oxidation at 60 g L-1 and optimal development at 30 g L-1. Endophytes were more evident for shoots from the CB origin. Explant sources responded distinctively to treatment due to physiological and intrinsic genetic factors. Therefore, explant sources, different culture media, sucrose concentration and p H may determine micropropagation success and influence the presence and/or intensity of endophytic manifestation. 展开更多
关键词 Plant TISSUE CULTURE EXPLANT sources CULTURE media SUCROSE pH
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Mini-cutting technique for Khaya anthotheca: selection of suitable IBA concentration and nutrient solution for its vegetative propagation
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作者 Joamir Barbosa Filho Maria Angélica Di Carvalho +2 位作者 leandro silva de oliveira Enéas Ricardo Konzen Gilvano Ebling Brondani 《Journal of Forestry Research》 SCIE CAS CSCD 2018年第1期73-84,共12页
Khaya anthotheca is a hardwood species from Africa and recently introduced to Brazil. This species yields high-quality wood for diverse applications, but little has been done toward an effective propagation method for... Khaya anthotheca is a hardwood species from Africa and recently introduced to Brazil. This species yields high-quality wood for diverse applications, but little has been done toward an effective propagation method for large-scale production in nurseries. We evaluated the effects of different concentrations of macro and micronutrients(i.e., 100, 50 and 25% of the concentration of a referenced solution) combined with indole-3-butyric acid(IBA) at 0 and 2 g L-1 on the survival rate of mini-stumps and the survival and adventitious rooting of K. anthotheca mini-cuttings. The mini-stumps were grown from a source of seeds imported from Ghana, West Africa. The ministumps survived at a high rate(97%). Consistently high shoot yields were obtained from the mini-stumps(average of 1.8 per mini-stump). High survival percentage of minicuttings and adventitious rooting were observed when solutions with 100 and 50% of the nutrients concentration were combined with 2 g L-1 IBA. Histological sections analyzed through optical microscopy indicated the tissues from mini-cutting stems were juvenile, which might have stimulated adventitious rooting. These results have important implications for further work aimed at establishing propagation strategies for K. anthotheca, which are of prime importance for assisting breeding programs of this species. 展开更多
关键词 African mahogany Seminal mini-garden Adventitious rooting Juvenile tissue Mini-stump
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