Over the course of civilization,humans have increasingly expanded their freedom to live a better life.In comparison with the primitive society,our modern society has many more choices of life-supporting resources,such...Over the course of civilization,humans have increasingly expanded their freedom to live a better life.In comparison with the primitive society,our modern society has many more choices of life-supporting resources,such as yearround food supply,permanent shelters,diverse energy sources,and effective preventive and curing medicine.However,our society is currently still heavily relying on the resources provided by Mother Nature,which cannot meet the future global needs in terms of both quantity and quality under the pressure of population growth,natural resource reduction,and environmental deterioration.For example,the food sources originating from plants,animals,or microbes do not have the nutrition balance for optimal human health[1–3].Climate change and environmental deterioration threaten the food security[4–6].Increasingly,infectious diseases(e.g.,HIV/AIDS),genetic diseases(e.g.,cancer),and improper lifestyle-related disorders(e.g.,obesity)become more prevalent and remain challenging to be prevented,controlled,and cured.Conventional medical technologies and modern medicine development are also meeting the ceiling.展开更多
Development of CRISPR-based epigenome editing tools is important for the study and engineering of biological behavior.Here,we describe the design of a reporter system for quantifying the ability of CRISPR epigenome ed...Development of CRISPR-based epigenome editing tools is important for the study and engineering of biological behavior.Here,we describe the design of a reporter system for quantifying the ability of CRISPR epigenome editors to produce a stable gene repression.We characterize the dynamics of durable gene silencing and reactivation,as well as the induced epigenetic changes of this system.We report the creation of single-protein CRISPR constructs bearing combinations of three epigenetic editing domains,termed KAL,that can stably repress the gene expression.This system should allow for the development of novel epigenome editing tools which will be useful in a wide array of biological research and engineering applications.展开更多
基金UT-Battelle,LLC under Contract No.DE-AC05-00OR22725 with the U.S.Department of Energy.Oak Ridge National Laboratory is managed by UT-Battelle,LLC for the U.S.Department of Energy under Contract Number DE-AC05-00OR22725.+1 种基金L.S.Q.is supported by the Department of Defense and Li Ka Shing Foundation for the work.A.J.is supported by BBSRC grant EVO-ENGINE BB/P020615/1 and EPSRC-BBSRC(no.BB/M017982/1,WISB Center).Research in Cheng’s Lab is supported by the Tennessee Agricultural Experiment Station Hatch Project TEN00491.
文摘Over the course of civilization,humans have increasingly expanded their freedom to live a better life.In comparison with the primitive society,our modern society has many more choices of life-supporting resources,such as yearround food supply,permanent shelters,diverse energy sources,and effective preventive and curing medicine.However,our society is currently still heavily relying on the resources provided by Mother Nature,which cannot meet the future global needs in terms of both quantity and quality under the pressure of population growth,natural resource reduction,and environmental deterioration.For example,the food sources originating from plants,animals,or microbes do not have the nutrition balance for optimal human health[1–3].Climate change and environmental deterioration threaten the food security[4–6].Increasingly,infectious diseases(e.g.,HIV/AIDS),genetic diseases(e.g.,cancer),and improper lifestyle-related disorders(e.g.,obesity)become more prevalent and remain challenging to be prevented,controlled,and cured.Conventional medical technologies and modern medicine development are also meeting the ceiling.
基金supported by the Stanford School of Medicine Dean’s Postdoctoral Fellowship.A.E.I.was supported by Stanford ChEM-H Undergraduate Research Fellow and Bio-X REU programs.Y.G.was supported by the National Science Foundation Graduate Research Fellowship Program.L.S.Q.acknowledges support from the Pew Charitable Trusts,the Alfred P.Sloan Foundation,and the Li Ka Shing Foundationby the Li Ka Shing Foundation and partly supported by the National Institutes of Health Common Fund 4D Nucleome Program(U01 DK127405).
文摘Development of CRISPR-based epigenome editing tools is important for the study and engineering of biological behavior.Here,we describe the design of a reporter system for quantifying the ability of CRISPR epigenome editors to produce a stable gene repression.We characterize the dynamics of durable gene silencing and reactivation,as well as the induced epigenetic changes of this system.We report the creation of single-protein CRISPR constructs bearing combinations of three epigenetic editing domains,termed KAL,that can stably repress the gene expression.This system should allow for the development of novel epigenome editing tools which will be useful in a wide array of biological research and engineering applications.
