New concept for the development of supramolecular assemblies from intricate inter-actions between different classes of biomacromolecules(polysaccharides,proteins and lipids)is yet to come,due to their intrinsic chemic...New concept for the development of supramolecular assemblies from intricate inter-actions between different classes of biomacromolecules(polysaccharides,proteins and lipids)is yet to come,due to their intrinsic chemical and structural complex-ity and incompatibility.Herein,we report an interaction mechanism among multiple biomacromolecules,and the structural and digestive properties of their assemblies using amylose(AM),lauric acid(LA),andβ-lactoglobulin(βLG)as exemplars.AM,LA,andβLG interact to form a water-soluble ternary complex through van der Waals forces between AM and LA and high affinity binding between AM andβLG,which can further assemble into uniform-sized,semi-crystalline nanospheres under certain thermodynamic conditions.These nanospheres are substantially resis-tant to amylolysis,thus can be well utilized by gut microbiota,including increasing short-chain fatty acid levels and shaping bacterial communities.Illustrating the com-plexation of AM,LA,andβLG and their assemblies from disorder to order,this work offers potential rationale of assemblies for multiple biomacromolecules driven by non-covalent interactions and substantial potentials for supramolecular biomaterials development.展开更多
基金Natural Science Foundation of Tianjin Municipal Science and Technology Commission,Grant/Award Number:20ZYJDJC00040National Natural Science Foundation of China,Grant/Award Numbers:32030084,32202053,U22A20545。
文摘New concept for the development of supramolecular assemblies from intricate inter-actions between different classes of biomacromolecules(polysaccharides,proteins and lipids)is yet to come,due to their intrinsic chemical and structural complex-ity and incompatibility.Herein,we report an interaction mechanism among multiple biomacromolecules,and the structural and digestive properties of their assemblies using amylose(AM),lauric acid(LA),andβ-lactoglobulin(βLG)as exemplars.AM,LA,andβLG interact to form a water-soluble ternary complex through van der Waals forces between AM and LA and high affinity binding between AM andβLG,which can further assemble into uniform-sized,semi-crystalline nanospheres under certain thermodynamic conditions.These nanospheres are substantially resis-tant to amylolysis,thus can be well utilized by gut microbiota,including increasing short-chain fatty acid levels and shaping bacterial communities.Illustrating the com-plexation of AM,LA,andβLG and their assemblies from disorder to order,this work offers potential rationale of assemblies for multiple biomacromolecules driven by non-covalent interactions and substantial potentials for supramolecular biomaterials development.
