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Human Nail Clippings as a Source of DNA for Genetic Studies 被引量:1
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作者 Le Truong Hannah Lui Park +5 位作者 Seong Sil Chang Argyrios Ziogas Susan L. Neuhausen Sophia S. Wang leslie bernstein Hoda Anton-Culver 《Open Journal of Epidemiology》 2015年第1期41-50,共10页
Blood samples have traditionally been used as the main source of DNA for genetic analysis. How-ever, this source can be difficult in terms of collection, transportation, and long-term storage. In this study, we invest... Blood samples have traditionally been used as the main source of DNA for genetic analysis. How-ever, this source can be difficult in terms of collection, transportation, and long-term storage. In this study, we investigated whether human nail clippings could be used as a source of DNA for SNP genotyping, null-allele detection, and whole-genome amplification. From extracted nail DNA, we achieved amplicons up to a length of ~400 bp and >96% concordance for SNP genotyping and 100% concordance for null-allele detection compared to DNA derived from matched blood sam-ples. For whole-genome amplification, OmniPlex performed better than Multiple Displacement Amplification with a success rate of 89.3% and 76.8% for SNP genotyping and null-allele detection, respectively. Concordance was ~98% for both methods. When combined with OmniPlex whole-genome amplification, human nail clippings could potentially be used as an alternative to whole blood as a less invasive and more convenient source of DNA for genotyping studies. 展开更多
关键词 Single NUCLEOTIDE Polymorphism (SNP) NAIL Clippings GENOTYPING WHOLE Genome AMPLIFICATION (WGA)
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