Experiment was designed to assess alimentary security or any beneficial effect on calve of a multispecies multistrain probiotic (MMP). An experiment with 36 calves, two day old, was conducted to assess the influence o...Experiment was designed to assess alimentary security or any beneficial effect on calve of a multispecies multistrain probiotic (MMP). An experiment with 36 calves, two day old, was conducted to assess the influence of probiotic on growth and health indicators. The treatment period was extended to 45 d. Group 1 received one daily dose of MMP (1.1 × 109 CFU per calf) during 20 d. Group 2 was the untreated control. On a weekly basis, every calf in each group was weighed to determine weight gain. Forty five days after the beginning of the experiment, blood samples were obtained from seven animals from Group 1 and six from Group 2, and peripheral blood neutrophils separated in order to determine metabolic and microbicidal activity. There was a significant increase in H2O2 production and NBT reduction test in MMP treated calves. The MMP not only lacks adverse effects when supplied as food additive, but showed health benefits. The prevention of infection and the highly significant increase of phagocytic activity in peripheral blood leukocytes seen in calves strongly suggest an efficient connection between the MMP and the immune system.展开更多
The aims of this work were: To achieve a simple and low cost propagation of potential probiotic agents using plain whey as a culture medium, study the diversity of the members of the bacterial community (MC) in plain ...The aims of this work were: To achieve a simple and low cost propagation of potential probiotic agents using plain whey as a culture medium, study the diversity of the members of the bacterial community (MC) in plain whey and to evaluate the probiotic capacity of this MC. After a systematic selection of agents according to their growing capacity in whey, the constituted MC was considered as a unit. Biochemical characterization of the lactic acid bacteria were performed using the API system. Molecular characterization of the lactic acid bacteria was realized using AFLPTM DNA-fingerprinting, partial 16S rDNA sequence analysis and PCR-denaturing gradient gel electrophoresis (PCR-DGGE). The physiological characterization of yeast was determined with the automated microplate method Allev/Biolomics and using yeast characterization system based on standard taxonomic criteria. The identification molecular was realized by PCR-fingerprinting. The resistance of MC to pH and bile salts were evaluated. The MC was composed of agents from different separated Dominium like Bacteria (Lactobacillum) and Eukaria (yeast). They are multispecies and also multistrain assuring high biodiversity. The MC grew at low pH and different concentrations bile salts.展开更多
文摘Experiment was designed to assess alimentary security or any beneficial effect on calve of a multispecies multistrain probiotic (MMP). An experiment with 36 calves, two day old, was conducted to assess the influence of probiotic on growth and health indicators. The treatment period was extended to 45 d. Group 1 received one daily dose of MMP (1.1 × 109 CFU per calf) during 20 d. Group 2 was the untreated control. On a weekly basis, every calf in each group was weighed to determine weight gain. Forty five days after the beginning of the experiment, blood samples were obtained from seven animals from Group 1 and six from Group 2, and peripheral blood neutrophils separated in order to determine metabolic and microbicidal activity. There was a significant increase in H2O2 production and NBT reduction test in MMP treated calves. The MMP not only lacks adverse effects when supplied as food additive, but showed health benefits. The prevention of infection and the highly significant increase of phagocytic activity in peripheral blood leukocytes seen in calves strongly suggest an efficient connection between the MMP and the immune system.
文摘The aims of this work were: To achieve a simple and low cost propagation of potential probiotic agents using plain whey as a culture medium, study the diversity of the members of the bacterial community (MC) in plain whey and to evaluate the probiotic capacity of this MC. After a systematic selection of agents according to their growing capacity in whey, the constituted MC was considered as a unit. Biochemical characterization of the lactic acid bacteria were performed using the API system. Molecular characterization of the lactic acid bacteria was realized using AFLPTM DNA-fingerprinting, partial 16S rDNA sequence analysis and PCR-denaturing gradient gel electrophoresis (PCR-DGGE). The physiological characterization of yeast was determined with the automated microplate method Allev/Biolomics and using yeast characterization system based on standard taxonomic criteria. The identification molecular was realized by PCR-fingerprinting. The resistance of MC to pH and bile salts were evaluated. The MC was composed of agents from different separated Dominium like Bacteria (Lactobacillum) and Eukaria (yeast). They are multispecies and also multistrain assuring high biodiversity. The MC grew at low pH and different concentrations bile salts.
