Osteoarthritis is a complex degenerative disease that affects the entire joint tissue.Currently,non-surgical treatments for osteoarthritis focus on relieving pain.While end-stage osteoarthritis can be treated with art...Osteoarthritis is a complex degenerative disease that affects the entire joint tissue.Currently,non-surgical treatments for osteoarthritis focus on relieving pain.While end-stage osteoarthritis can be treated with arthroplasty,the health and financial costs associated with surgery have forced the search for alternative non-surgical treatments to delay the progression of osteoarthritis and promote cartilage repair.Unlike traditional treatment,the gene therapy approach allows for long-lasting expression of therapeutic proteins at specific sites.In this review,we summarize the history of gene therapy in osteoarthritis,outlining the common expression vectors(non-viral,viral),the genes delivered(transcription factors,growth factors,inflammation-associated cytokines,non-coding RNAs)and the mode of gene delivery(direct delivery,indirect delivery).We highlight the application and development prospects of the gene editing technology CRISPR/Cas9 in osteoarthritis.Finally,we identify the current problems and possible solutions in the clinical translation of gene therapy for osteoarthritis.展开更多
基金supported by the National Natural Science Foundation of China(NSFC)to ZYH(Grants No.92049101,81972097,and 81702185)This research was also supported by Sichuan Science and Technology Program to ZYH(Grant No.2022YFH0101)+1 种基金This work was also supported by Guangdong Basic and Applied Basic Research Foundation(Grant No.2021A1515220030)Shenzhen Science and Technology Program(Grant No.RCYX20210609103902019)。
文摘Osteoarthritis is a complex degenerative disease that affects the entire joint tissue.Currently,non-surgical treatments for osteoarthritis focus on relieving pain.While end-stage osteoarthritis can be treated with arthroplasty,the health and financial costs associated with surgery have forced the search for alternative non-surgical treatments to delay the progression of osteoarthritis and promote cartilage repair.Unlike traditional treatment,the gene therapy approach allows for long-lasting expression of therapeutic proteins at specific sites.In this review,we summarize the history of gene therapy in osteoarthritis,outlining the common expression vectors(non-viral,viral),the genes delivered(transcription factors,growth factors,inflammation-associated cytokines,non-coding RNAs)and the mode of gene delivery(direct delivery,indirect delivery).We highlight the application and development prospects of the gene editing technology CRISPR/Cas9 in osteoarthritis.Finally,we identify the current problems and possible solutions in the clinical translation of gene therapy for osteoarthritis.
