Objective： The activation of hedgehog （HH） pathway is implicated in the development of human malignancies including hepatocellular carcinoma （HCC）. However, the clinical impact of HH activation in HCC patients is...Objective： The activation of hedgehog （HH） pathway is implicated in the development of human malignancies including hepatocellular carcinoma （HCC）. However, the clinical impact of HH activation in HCC patients is still unclear. This study was conducted to confirm whether the expression of HH pathway components was associated with HCC progression and clinical outcome. Methods： This study was a sample-expanded and prolonged follow up of one of our previous studies. It included 46 HCC patients who underwent surgical treatment from 2002 to 2005. The expression of sonic HH （SHIq）, patched-1 （PTCHI）, smoothened （SMOH） and glioma-associated oncogene-1 （GLI1） genes in tumor and adjacent normal tissues extracted from the patients were examined by reverse transcription- polymerase chain reaction （RT-PCR） to explore the relationship between these genes and the clinical prognosis of HCC. Results： The expression levels ofSHH, PTCH1, SMOH and GLI1 in HCC tissues were 60.87%, 50.00%, 32.61% and 54.35%, respectively. The expression levels of SHH-related molecules were relatively intense in cancer tissue, but insignificantly correlated with any clinicopathological factors of tumor. Transcriptional factor GLI1 was the only molecule associated with poor prognosis among the HCC patients. The expression of GLI1 gene in tumor tissues was significantly related with disease-free survival （DFS） （P=0.042） and overall survival （OS） （P=0.030）. The simultaneous expression of GLI1 in tumor and adjacent normal liver tissues correlated with DFS （P〈0.029） and OS （P〈0.025）. Conclusions： HH signaling activation is an important event in the development of human HCC. The expression of GLI1 in SHH pathway is possibly involved in HCC progression, which may be a useful prognostic indicator of HCC.展开更多
Objective：To evaluate the effectiveness and safety of the mobilization of peripheral blood hematopoietic stem cells by combining docetaxel with granulocyte colony-stimulating factor（G-CSF） in breast cancer patients...Objective：To evaluate the effectiveness and safety of the mobilization of peripheral blood hematopoietic stem cells by combining docetaxel with granulocyte colony-stimulating factor（G-CSF） in breast cancer patients.Methods：A total of 57 breast cancer patients were treated with docetaxel 120 mg/m2.When the white blood cell（WBC） count decreased to 1.0×109/L,patients were given G-CSF 5-g/kg daily by subcutaneous injection until the end of apheresis.Peripheral blood mononuclear cells（MNC） were isolated by Cobe Spectra Apheresis System.The percentage of CD34＋ cell was assayed by flow cytometry.Results：At a median 6 of days（range 3-8） after the administration of docetaxel,the median WBC count decreased to 1.08×109/L（range 0.20-2.31）.The median duration of G-CSF mobilization was 3 days（range 2-7）.The MNC collection was conducted 8-12 days（median 10 days） after docetaxel treatment.The median MNC was 5.35×108/kg（range 0.59-14.07）,the median CD34＋ cell count was 2.43×106/kg（range 0.16-16.69）.The CD34＋ cell count was higher than 1.00×106/kg in 47 of 57 cases（82.46%） and higher than 2.00×106/kg in 36 cases（63.16%）.The CD34＋ cell count was higher than 2.00×106/kg in 27 collections（23.68%）.The MNC count and the CD34＋ cell count were correlated with the bottom of WBC after docetaxel chemotherapy（r=0.364,0.502,P=0.005,0.000）.The CD34＋ cell count was correlated with the MNC count（r=0.597,P=0.000）.The mobilization and apheresis were well tolerated in all patients.Mild perioral numbness and numbness of hand or feet were observed in 3 cases.No serious adverse events were reported.Conclusion：Mobilization of peripheral blood hematopoietic stem cell by combining docetaxel with G-CSF was effective and safety in breast cancer patients.展开更多
AIM: To investigate whether IDH1R132 C mutant in combination with loss of p53 and activated Notch signaling promotes intrahepatic cholangiocarcinoma(ICC) development.METHODS: We applied hydrodynamic injection and slee...AIM: To investigate whether IDH1R132 C mutant in combination with loss of p53 and activated Notch signaling promotes intrahepatic cholangiocarcinoma(ICC) development.METHODS: We applied hydrodynamic injection and sleeping beauty mediated somatic integration to induce loss of p53(via sh P53), activation of Notch [via intracellular domain of Notch1(NICD)] and/or overexpression of IDH1R132 C mutant together with the sleeping beauty transposase into the mouse liver. Specifically, we co-expressed sh P53 and NICD(sh P53/NICD, n = 4), sh P53 and IDH1R132C(sh P53/IDH1R132 C, n = 3), NICD and IDH1R132C(NICD/IDH1R132 C, n = 4), as well as NICD, sh P53 and IDH1R132C(NICD/sh P53/IDH1R132 C, n = 9) in mice. Mice were monitored for liver tumor development and euthanized at various time points. Liver histology was analyzed by hematoxylin and eosin staining. Molecular features of NICD/sh P53/IDH1R132 C ICC tumor cells were characterized by Myc tag, Flag tag, Ki-67, p-Erk and p-AKT immunohistochemical staining. Desmoplastic reaction in tumor tissues was studied by Picro-Sirius red staining.RESULTS: We found that co-expression of sh P53/NICD, sh P53/IDH1R132 C or NICD/IDH1R132 C did not lead to liver tumor formation. In striking contrast, coexpression of NICD/sh P53/IDH1R132 C resulted in ICC development in mice(P < 0.01). The tumors could be identified as early as 12 wk post hydrodynamic injection. Tumors rapidly progressed, and by 18 wk post hydrodynamic injection, multiple cystic lesions could be identified on the liver surface. NICD/sh P53/IDH1R132 C liver tumors shared multiple histological features of human ICCs, including hyperplasia of irregular glands. Importantly, all tumor cells were positive for the biliary epithelial cell marker cytokeratin 19. Extensive collagen fibers could be visualized in tumor tissues using Sirus red staining, duplicating the desmoplastic reaction observed in human ICC. Tumors were highly proliferative and expressed ectopically injected genes. Together these studies supported that NICD/sh P53/IDH1R132 C liver tumors were indeed ICCs. Finally, no p-AKT or p-ERK positive staining was observed, suggesting that NICD/sh P53/IDH1R132 C driven ICC development was independent of AKT/m TOR and Ras/MAPK signaling cascades. CONCLUSION: We have generated a simple, nongermline murine ICC model with activated Notch, loss of p53 and IDH1R132 C mutant. The study supported the oncogenic potential of IDH1R132 C.展开更多
Due to outstanding mechanical properties, heat resistance, and relatively facile production, nanoclay reinforced epoxy composites (NCRE composites) have been suggested as candi- date materials for use on external surf...Due to outstanding mechanical properties, heat resistance, and relatively facile production, nanoclay reinforced epoxy composites (NCRE composites) have been suggested as candi- date materials for use on external surfaces of spacecraft residing in the low Earth orbit (LEO) environment. The resistance of the NCRE composites to bombardment by atomic oxygen (AO), a dominant component of the LEO environment, has been investigated. Four types of samples were used in this study. They were pure epoxy (0 wt% nanoclay con- tent), and NCRE composites with di erent loadings of nanoclay|1 wt%, 2 wt%, and 4 wt%. Etch depths decreased with increasing nanoclay content, and for the 4 wt% samples it ranged from 28% to 37% compared to that of pure epoxy. X-ray photoelectron spectroscopy (XPS) indicates that after AO bombardment, relative area of C-C/C-H peak decreased, while the area of the C-O, ketones peaks increased, and the oxidation degree of surfaces increased. New carbon-related component carbonates were detected on nanoclay contain- ing composite surfaces. Scanning electron microscopy indicates that aggregates formed on nanoclay-containing surfaces after AO bombardment. The sizes and densities of aggregates increased with nanoclay content. The combined erosion depths, XPS and SEM results indi- cate that although all the studied surfaces got eroded and oxidized after AO bombardment, the nanoclay containing composites showed better AO resistance compared to pure epoxy, because the produced aggregates on surface potentially act as a physical “shield”, e ectively retarding parts of the surface from further AO etching.展开更多
With the continuous development of industry and the progress of science and technology, energy resources consumption becomes increasingly serious. Under the sustainable development of environment, energy saving, envir...With the continuous development of industry and the progress of science and technology, energy resources consumption becomes increasingly serious. Under the sustainable development of environment, energy saving, environmental protection requirements improve continuously. As an energy-intensive industry occupying one third of the total energy consumption, the construction industry’s energy consumption problem becomes increasingly serious. In order to promote human ecological balance and sustainable development of society, combining the ecological concept into the architectural design is imminent. The so-called ecological architecture is based on the nature, guided by sustainable development, and combines the ecological theory into the architecture. This paper briefl y introduces the ecological architecture, discusses the application of ecological ideas in architectural design and its design principle, which plays an important role in the ecological construction’s integrating in architectural design.展开更多
Recent developments in 3D graphics technology have led to extensive processes on 3D meshes(e.g.,compression,simplification,transmission and watermarking),these processes unavoidably cause the visual perceptual degrada...Recent developments in 3D graphics technology have led to extensive processes on 3D meshes(e.g.,compression,simplification,transmission and watermarking),these processes unavoidably cause the visual perceptual degradation of the 3D objects.The existing mesh visual quality evaluation metrics either require topology constrain or fail to reflect the perceived visual quality.Meanwhile,for the 3D objects that are observed on 2D screens by the users,it is reasonable to apply image metric to assess the distortion caused by mesh simplification.We attempt to explore the efficiency of image metric for assessing the visual fidelity of the simplified 3D model in this paper.For this purpose,several latest and most effective image metrics,2D snapshots,number and pooling algorithms are involved in our study,and finally tested on the IEETA simplification database.The statistical data allow the researcher to select the optimal parameter for this image-based mesh visual quality assessment and provide a new perspective for the design and performance assessment of mesh simplification algorithms.展开更多
研究一类未知异构非线性多智能体的编队控制问题.首先,利用全格式动态线性化(full form dynamic linearization, FFDL)方法将未知非线性智能体转化为含有时变参数的数据模型,并给出时变参数的估计方法;然后,基于该数据模型设计一种分布...研究一类未知异构非线性多智能体的编队控制问题.首先,利用全格式动态线性化(full form dynamic linearization, FFDL)方法将未知非线性智能体转化为含有时变参数的数据模型,并给出时变参数的估计方法;然后,基于该数据模型设计一种分布式无模型自适应多智能体编队控制方案;最后,为验证所提出的无模型自适应编队控制方案的有效性,利用3台NAO机器人开发基于Python的多智能体编队控制实验平台.实验比较结果表明,通过所提出的控制方案可使3台机器人仅利用局部信息就能有效完成编队控制任务,控制性能优于基于PID的编队控制方法.展开更多
Chlamydia trachomatis （Ct） genital infection is the most common sexually transmitted disease （STD） in China and the US. The morbidity of Ct genital infection in China has increased from 32.48/100,000 in 2008 to 37...Chlamydia trachomatis （Ct） genital infection is the most common sexually transmitted disease （STD） in China and the US. The morbidity of Ct genital infection in China has increased from 32.48/100,000 in 2008 to 37.18/100,000 in 2015.[1] The major areas of Ct infections are concentrated in the Zhujiang Delta, Changjiang Delta, Minjiang Area, and West China. In these areas, the highest incidence of Ct infection reaches 615.99/100,000 citizens. In the US, there are 1,441,789 reported Ct, which include 627.2 females and 278.4 males per 100,000 population. It is now the most prevalent STD, with its rate increasing to 22% in males and 6% in females.[2] Ct genital infection can cause epididymitis, prostatitis, cervicitis, annexitis, infertility, and atopic pregnancy, which have been identified as the major public health problems.展开更多
基金supported by the Project 2009CB521700 of the National Basic Research Program of China ("973"Program)the Capital Development Grant (2007-2053)
文摘Objective： The activation of hedgehog （HH） pathway is implicated in the development of human malignancies including hepatocellular carcinoma （HCC）. However, the clinical impact of HH activation in HCC patients is still unclear. This study was conducted to confirm whether the expression of HH pathway components was associated with HCC progression and clinical outcome. Methods： This study was a sample-expanded and prolonged follow up of one of our previous studies. It included 46 HCC patients who underwent surgical treatment from 2002 to 2005. The expression of sonic HH （SHIq）, patched-1 （PTCHI）, smoothened （SMOH） and glioma-associated oncogene-1 （GLI1） genes in tumor and adjacent normal tissues extracted from the patients were examined by reverse transcription- polymerase chain reaction （RT-PCR） to explore the relationship between these genes and the clinical prognosis of HCC. Results： The expression levels ofSHH, PTCH1, SMOH and GLI1 in HCC tissues were 60.87%, 50.00%, 32.61% and 54.35%, respectively. The expression levels of SHH-related molecules were relatively intense in cancer tissue, but insignificantly correlated with any clinicopathological factors of tumor. Transcriptional factor GLI1 was the only molecule associated with poor prognosis among the HCC patients. The expression of GLI1 gene in tumor tissues was significantly related with disease-free survival （DFS） （P=0.042） and overall survival （OS） （P=0.030）. The simultaneous expression of GLI1 in tumor and adjacent normal liver tissues correlated with DFS （P〈0.029） and OS （P〈0.025）. Conclusions： HH signaling activation is an important event in the development of human HCC. The expression of GLI1 in SHH pathway is possibly involved in HCC progression, which may be a useful prognostic indicator of HCC.
基金supported by a grant from the Beijing Capital Development Foundation for Medical Sciences (No. 2007-2053)
文摘Objective：To evaluate the effectiveness and safety of the mobilization of peripheral blood hematopoietic stem cells by combining docetaxel with granulocyte colony-stimulating factor（G-CSF） in breast cancer patients.Methods：A total of 57 breast cancer patients were treated with docetaxel 120 mg/m2.When the white blood cell（WBC） count decreased to 1.0×109/L,patients were given G-CSF 5-g/kg daily by subcutaneous injection until the end of apheresis.Peripheral blood mononuclear cells（MNC） were isolated by Cobe Spectra Apheresis System.The percentage of CD34＋ cell was assayed by flow cytometry.Results：At a median 6 of days（range 3-8） after the administration of docetaxel,the median WBC count decreased to 1.08×109/L（range 0.20-2.31）.The median duration of G-CSF mobilization was 3 days（range 2-7）.The MNC collection was conducted 8-12 days（median 10 days） after docetaxel treatment.The median MNC was 5.35×108/kg（range 0.59-14.07）,the median CD34＋ cell count was 2.43×106/kg（range 0.16-16.69）.The CD34＋ cell count was higher than 1.00×106/kg in 47 of 57 cases（82.46%） and higher than 2.00×106/kg in 36 cases（63.16%）.The CD34＋ cell count was higher than 2.00×106/kg in 27 collections（23.68%）.The MNC count and the CD34＋ cell count were correlated with the bottom of WBC after docetaxel chemotherapy（r=0.364,0.502,P=0.005,0.000）.The CD34＋ cell count was correlated with the MNC count（r=0.597,P=0.000）.The mobilization and apheresis were well tolerated in all patients.Mild perioral numbness and numbness of hand or feet were observed in 3 cases.No serious adverse events were reported.Conclusion：Mobilization of peripheral blood hematopoietic stem cell by combining docetaxel with G-CSF was effective and safety in breast cancer patients.
基金supported by the National Key Research and Development Program of(No.2021YFA1500601 and No.2018YFA0208703)the National Natural Science Foundation of China(No.21973010 and No.21973092)+3 种基金the Instrument Developing Project of the Chinese Academy of Sciences(No.YZ201504)the CAS Projects for Young Scientists in Basic Research(No.YSBR-007)the Dalian Institute of Chemical Physics Innovation Foundation(DICP I202205)LiaoNing Revitalization Talents Program(No.XLYC1907032).
基金supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (No.XDB17000000)the National Natural Science Foundation of China (NSFC Center for Chemical Dynamics (No.21688102)+4 种基金the National Natural Science Foundation of China (No.21673232, No.21873099, No.21922306)the International Partnership Program of Chinese Academy of Sci-ences (No.121421KYSB20170012)supported by the National Natural Science Foundation of China (No.21973010)supported by the National Natural Science Foundation of China (No.21773236)supported by the Natural Science Research Project of Education Department of Anhui Province (No.KJ2019A0521).
基金Supported by Grants from National Institutes of HealthNo.R01CA136606(in part+5 种基金to Chen X)UCSF Liver CenterNo.P30DK026743China Scholarship CouncilcontractNo.201206010086(to Ding N)and No.201306590021(to Li XL)
文摘AIM: To investigate whether IDH1R132 C mutant in combination with loss of p53 and activated Notch signaling promotes intrahepatic cholangiocarcinoma(ICC) development.METHODS: We applied hydrodynamic injection and sleeping beauty mediated somatic integration to induce loss of p53(via sh P53), activation of Notch [via intracellular domain of Notch1(NICD)] and/or overexpression of IDH1R132 C mutant together with the sleeping beauty transposase into the mouse liver. Specifically, we co-expressed sh P53 and NICD(sh P53/NICD, n = 4), sh P53 and IDH1R132C(sh P53/IDH1R132 C, n = 3), NICD and IDH1R132C(NICD/IDH1R132 C, n = 4), as well as NICD, sh P53 and IDH1R132C(NICD/sh P53/IDH1R132 C, n = 9) in mice. Mice were monitored for liver tumor development and euthanized at various time points. Liver histology was analyzed by hematoxylin and eosin staining. Molecular features of NICD/sh P53/IDH1R132 C ICC tumor cells were characterized by Myc tag, Flag tag, Ki-67, p-Erk and p-AKT immunohistochemical staining. Desmoplastic reaction in tumor tissues was studied by Picro-Sirius red staining.RESULTS: We found that co-expression of sh P53/NICD, sh P53/IDH1R132 C or NICD/IDH1R132 C did not lead to liver tumor formation. In striking contrast, coexpression of NICD/sh P53/IDH1R132 C resulted in ICC development in mice(P < 0.01). The tumors could be identified as early as 12 wk post hydrodynamic injection. Tumors rapidly progressed, and by 18 wk post hydrodynamic injection, multiple cystic lesions could be identified on the liver surface. NICD/sh P53/IDH1R132 C liver tumors shared multiple histological features of human ICCs, including hyperplasia of irregular glands. Importantly, all tumor cells were positive for the biliary epithelial cell marker cytokeratin 19. Extensive collagen fibers could be visualized in tumor tissues using Sirus red staining, duplicating the desmoplastic reaction observed in human ICC. Tumors were highly proliferative and expressed ectopically injected genes. Together these studies supported that NICD/sh P53/IDH1R132 C liver tumors were indeed ICCs. Finally, no p-AKT or p-ERK positive staining was observed, suggesting that NICD/sh P53/IDH1R132 C driven ICC development was independent of AKT/m TOR and Ras/MAPK signaling cascades. CONCLUSION: We have generated a simple, nongermline murine ICC model with activated Notch, loss of p53 and IDH1R132 C mutant. The study supported the oncogenic potential of IDH1R132 C.
基金the National Natu- ral Science Foundation of China (No.21473015 and No.41574101)the Fundamental Research Funds for the Central Universities (No.3132018233).
文摘Due to outstanding mechanical properties, heat resistance, and relatively facile production, nanoclay reinforced epoxy composites (NCRE composites) have been suggested as candi- date materials for use on external surfaces of spacecraft residing in the low Earth orbit (LEO) environment. The resistance of the NCRE composites to bombardment by atomic oxygen (AO), a dominant component of the LEO environment, has been investigated. Four types of samples were used in this study. They were pure epoxy (0 wt% nanoclay con- tent), and NCRE composites with di erent loadings of nanoclay|1 wt%, 2 wt%, and 4 wt%. Etch depths decreased with increasing nanoclay content, and for the 4 wt% samples it ranged from 28% to 37% compared to that of pure epoxy. X-ray photoelectron spectroscopy (XPS) indicates that after AO bombardment, relative area of C-C/C-H peak decreased, while the area of the C-O, ketones peaks increased, and the oxidation degree of surfaces increased. New carbon-related component carbonates were detected on nanoclay contain- ing composite surfaces. Scanning electron microscopy indicates that aggregates formed on nanoclay-containing surfaces after AO bombardment. The sizes and densities of aggregates increased with nanoclay content. The combined erosion depths, XPS and SEM results indi- cate that although all the studied surfaces got eroded and oxidized after AO bombardment, the nanoclay containing composites showed better AO resistance compared to pure epoxy, because the produced aggregates on surface potentially act as a physical “shield”, e ectively retarding parts of the surface from further AO etching.
基金supported by the National Natural Science Foundation of China (No.21473015, No.21203016, No.41574101, No.21673232, No.21773213)the Foundation for the Author of National Excellent Doctoral Dissertation of China (No.201222)
基金V. ACKNOWLEDGMENTS This work was supported by the Chinese Academy of Sciences, the Ministry of Science and Technology, and the National Natural Science Foundation of China.
文摘With the continuous development of industry and the progress of science and technology, energy resources consumption becomes increasingly serious. Under the sustainable development of environment, energy saving, environmental protection requirements improve continuously. As an energy-intensive industry occupying one third of the total energy consumption, the construction industry’s energy consumption problem becomes increasingly serious. In order to promote human ecological balance and sustainable development of society, combining the ecological concept into the architectural design is imminent. The so-called ecological architecture is based on the nature, guided by sustainable development, and combines the ecological theory into the architecture. This paper briefl y introduces the ecological architecture, discusses the application of ecological ideas in architectural design and its design principle, which plays an important role in the ecological construction’s integrating in architectural design.
文摘Recent developments in 3D graphics technology have led to extensive processes on 3D meshes(e.g.,compression,simplification,transmission and watermarking),these processes unavoidably cause the visual perceptual degradation of the 3D objects.The existing mesh visual quality evaluation metrics either require topology constrain or fail to reflect the perceived visual quality.Meanwhile,for the 3D objects that are observed on 2D screens by the users,it is reasonable to apply image metric to assess the distortion caused by mesh simplification.We attempt to explore the efficiency of image metric for assessing the visual fidelity of the simplified 3D model in this paper.For this purpose,several latest and most effective image metrics,2D snapshots,number and pooling algorithms are involved in our study,and finally tested on the IEETA simplification database.The statistical data allow the researcher to select the optimal parameter for this image-based mesh visual quality assessment and provide a new perspective for the design and performance assessment of mesh simplification algorithms.
文摘研究一类未知异构非线性多智能体的编队控制问题.首先,利用全格式动态线性化(full form dynamic linearization, FFDL)方法将未知非线性智能体转化为含有时变参数的数据模型,并给出时变参数的估计方法;然后,基于该数据模型设计一种分布式无模型自适应多智能体编队控制方案;最后,为验证所提出的无模型自适应编队控制方案的有效性,利用3台NAO机器人开发基于Python的多智能体编队控制实验平台.实验比较结果表明,通过所提出的控制方案可使3台机器人仅利用局部信息就能有效完成编队控制任务,控制性能优于基于PID的编队控制方法.
基金This work was supported by a grant from the Natural Science Foundation of China(No.31370211).
文摘Chlamydia trachomatis （Ct） genital infection is the most common sexually transmitted disease （STD） in China and the US. The morbidity of Ct genital infection in China has increased from 32.48/100,000 in 2008 to 37.18/100,000 in 2015.[1] The major areas of Ct infections are concentrated in the Zhujiang Delta, Changjiang Delta, Minjiang Area, and West China. In these areas, the highest incidence of Ct infection reaches 615.99/100,000 citizens. In the US, there are 1,441,789 reported Ct, which include 627.2 females and 278.4 males per 100,000 population. It is now the most prevalent STD, with its rate increasing to 22% in males and 6% in females.[2] Ct genital infection can cause epididymitis, prostatitis, cervicitis, annexitis, infertility, and atopic pregnancy, which have been identified as the major public health problems.
