The basal ganglia is a critical subcortical nuclei in several parallel and closed circuits,which mainly modulate thalamus-cortex activity.Dysfunction in any one of these loops connecting cortex can lead to movement an...The basal ganglia is a critical subcortical nuclei in several parallel and closed circuits,which mainly modulate thalamus-cortex activity.Dysfunction in any one of these loops connecting cortex can lead to movement and/or mood disorders.To understand cellular complexity,differential gene expression and transcriptional regulatory network of human basal ganglia under normal condition,we performed single cell RNA sequencing of 2424 cells sampled from the basal ganglia of human postmortem brains including caudate,putamen,substantia nigra and globus pallidus.展开更多
With the advent of single cell technology and its rapid applications in the biomedical field,it becomes possible to identify,categorize and characterize each single cell in the brain.Shanghai Brain Bank is accumulatin...With the advent of single cell technology and its rapid applications in the biomedical field,it becomes possible to identify,categorize and characterize each single cell in the brain.Shanghai Brain Bank is accumulating donorbrains very fast(estimately 80 brains till now)from people with normal aging or various neurodegenerative diseases.The basal ganglia is critical for movement control,behavior,cognition,emotion and reward.Many neurodegenerative diseases are related to the abnormality or aging of human basal ganglia.Single nuclei transcriptomic profiles of both normal and diseased human basal ganglia are still sparse.In our study,we systematically examined different nuclei(caudate,putamen,globus pallidus,and substantia nigra)in human basal ganglia from 6 control and 5 diseased donors(2 psychiatric diseases,2 PD and 1 AD)using combinatory indexing single nuclei RNA sequencing.Gene expression was measured on a total of 22309 control and 42590 diseased single cells.展开更多
为降低以中密度纤维板(MDF)为表板的三聚氰胺浸渍纸贴面实木复合门扇制作和使用过程中的变形率,提高其结构稳定性,设计制作2组共6种不同表板结构的门扇进行对照测试。通过对翘曲度和表板含水率的测量分析,结果表明:双层和双贴面MDF表板...为降低以中密度纤维板(MDF)为表板的三聚氰胺浸渍纸贴面实木复合门扇制作和使用过程中的变形率,提高其结构稳定性,设计制作2组共6种不同表板结构的门扇进行对照测试。通过对翘曲度和表板含水率的测量分析,结果表明:双层和双贴面MDF表板结构均能增强门扇抗变形能力,对比常规门扇,测试中的双贴面4.5 mm MDF和5.7 mm MDF表板门扇7 d翘曲度均值分别降低71%和66%,单贴面双层3 mm普通和防潮MDF表板门扇降低29%和50%;单贴面4.5 mm MDF表板门扇7 d翘曲度均值升高25%;7 d观测期内表板含水率分布差异和含水率变化速度与门扇变形呈现相关性,测试中双贴面5.7 mm MDF表板门扇两项数据综合最佳。展开更多
文摘The basal ganglia is a critical subcortical nuclei in several parallel and closed circuits,which mainly modulate thalamus-cortex activity.Dysfunction in any one of these loops connecting cortex can lead to movement and/or mood disorders.To understand cellular complexity,differential gene expression and transcriptional regulatory network of human basal ganglia under normal condition,we performed single cell RNA sequencing of 2424 cells sampled from the basal ganglia of human postmortem brains including caudate,putamen,substantia nigra and globus pallidus.
文摘With the advent of single cell technology and its rapid applications in the biomedical field,it becomes possible to identify,categorize and characterize each single cell in the brain.Shanghai Brain Bank is accumulating donorbrains very fast(estimately 80 brains till now)from people with normal aging or various neurodegenerative diseases.The basal ganglia is critical for movement control,behavior,cognition,emotion and reward.Many neurodegenerative diseases are related to the abnormality or aging of human basal ganglia.Single nuclei transcriptomic profiles of both normal and diseased human basal ganglia are still sparse.In our study,we systematically examined different nuclei(caudate,putamen,globus pallidus,and substantia nigra)in human basal ganglia from 6 control and 5 diseased donors(2 psychiatric diseases,2 PD and 1 AD)using combinatory indexing single nuclei RNA sequencing.Gene expression was measured on a total of 22309 control and 42590 diseased single cells.
文摘为降低以中密度纤维板(MDF)为表板的三聚氰胺浸渍纸贴面实木复合门扇制作和使用过程中的变形率,提高其结构稳定性,设计制作2组共6种不同表板结构的门扇进行对照测试。通过对翘曲度和表板含水率的测量分析,结果表明:双层和双贴面MDF表板结构均能增强门扇抗变形能力,对比常规门扇,测试中的双贴面4.5 mm MDF和5.7 mm MDF表板门扇7 d翘曲度均值分别降低71%和66%,单贴面双层3 mm普通和防潮MDF表板门扇降低29%和50%;单贴面4.5 mm MDF表板门扇7 d翘曲度均值升高25%;7 d观测期内表板含水率分布差异和含水率变化速度与门扇变形呈现相关性,测试中双贴面5.7 mm MDF表板门扇两项数据综合最佳。
