Comparative mitochondrial genome analysis of Cynoglossidae(Teleost:Pleuronectiformes)and phylogenetic implications

Generally,a teleostean group(e.g.,family or genus)owns one type or a set of similar mitochondrial gene arrangement.It is interesting,however,that four different types of gene arrangement have been found in the mitochondrial genome(mitogenome)of Cynoglossidae species.So far,the possible mechanisms of mitogenomic gene rearrangement and its potential implications have aroused widespread attention and caused lots of controversy.Here,a total of 21 Cynoglossidae mitogenomes and a newly sequenced mitogenome of Cynoglossus puncticpes(Pleuronectiformes:Cynoglossidae)were compared.The length ranges from 16417 bp to 18369 bp,which is mainly caused by the length heteroplasmy of control region(CR).Further analysis reveals that the difference of tandem repeats acts as a determining factor resulting in the length heterogeneity.Like most gene rearrangements of Cynoglossinae mitogenomes,tRNA-Gln gene encoded by the L-strand has translocated to the H-strand(Q inversion),accompanied by the translocation of CR in C.puncticpes mitogenome.The typical IQM order(tRNA-Ile-Gln-Met)changed to QIM order.Tandem duplication/random loss and mitochondrial recombination were accepted as the most possible models to account for the rearrangements in C.puncticpes mitogenome.Phylogenetic trees showed a strong correlation between the gap spacer in the rearranged QIM area and phylogeny,which provides a fresh idea for phylogenetic studies in future.

Two complete mitogenomes of Ocypodoidea(Decapoda: Brachyura), Cleistostoma dilatatum(Camptandriidae) and Euplax sp.(Macrophthalmidae) and its phylogenetic implications

Complete mitochondrial genomes(mitogenomes) can provide useful information for phylogenetic relationships,gene rearrangement, and molecular evolution. In the present study, two newly sequenced mitogenomes of Ocypodoidea(Cleistostoma dilatatum and Euplax sp.) were reported for the first time, which are 15 444 bp and16 129 bp in length, respectively. Cleistostoma dilatatum is the first species in the family Camptandriidae whose complete mitogenome was sequenced. Each mitogenome contains an entire set of 37 genes and a putative control region, but their gene arrangements are largely different. Tandem duplication and random loss model is proposed to account for their gene arrangements. Comparative genomic analyses of 19 mitogenomes clustering in one branch reveal that 18 of them shared the same gene rearrangement, while that of C. dilatatum mitogenome was consistent with the ancestral gene arrangement of Brachyura. The dN/dS ratio analysis shows that all PCGs are evolving under purifying selection. Phylogenetic analyses show that all Macrophalmidae species cluster together as a group, and then form a sister clade with Camptandriidae. Moreover, the polyphyly of three superfamilies(Ocypodoidea, Eriphioidea, and Grapsoidea) is reconfirmed. These findings help to confirm the phylogenetic position of Camptandriidae, as well as provide new insights into the phylogeny of Brachyura.

湿壁塔实验台控制系统的研发

随着二氧化碳排放问题的日益严峻,加速二氧化碳的减排计划已经刻不容缓。其中,对于二氧化碳吸附剂的研究是减排的重中之重。为了准确高效地研究二氧化碳吸附剂的传质特性,开发一套湿壁塔实验台控制系统,通过PLC对湿壁塔实验台的温度、压力和流速等参数进行数据采集与精确控制。详细介绍实验装置的工作原理与实验流程,以及组态软件的参数设定、远程控制、实时监控和报警等功能。该系统能够简化实验流程,提高实验效率,为吸附剂的研究提供可靠的理论和实践基础。

一种基于CommView的自定义解码器开发方案

针对通信协议的多样性,每次制定一种新协议,均需要相应的报文解码器验证协议的正确性,这就需要一种报文引擎,提供自定义协议的解码接口,而CommView满足自定义解码显示要求,因此提出了一种基于CommView的自定义解码器开发方案。方案以CommView开发自定义解码器为实例,调用CommView报文引擎,以实现IECGOOSE报文的解码、结构层次树显示为实例,介绍了具体的自定义解码器的开发流程。实际证明通过CommView开发自定义解码器,能够有效提高网络通信协议的开发调试效率。

Clonality and allelotype analyses of focal nodular hyperplasia compared with hepatocellular adenoma and carcinoma

AIM: To identify clonality and genetic alterations in focal nodular hyperplasia (FNH) and the nodules derived from it. METHODS: Twelve FNH lesions were examined. Twelve hepatocellular adenomas (HCAs) and 22 hepatocellular carcinomas (HCCs) were used as references. Nodules of different types were identified and isolated from FNH by microdissection. An X-chromosome inactivation assay was employed to describe their clonality status. Loss of heterozygosity (LOH) was detected, using 57 markers, for genetic alterations.RESULTS: Nodules of altered hepatocytes (NAH), the putative precursors of HCA and HCC, were found in all the FNH lesions. Polyclonality was revealed in 10 FNH lesions from female patients, and LOH was not detected in any of the six FNH lesions examined, the results apparently showing their polyclonal nature. In contrast, monoclonality was demonstrated in all the eight HCAs and in four of the HCCs from females, and allelic imbalances were found in the HCAs (9/9) and HCCs (15/18), with chromosomal arms 11p, 13q and 17p affected in the former, and 6q, 8p, 11p, 16q and 17p affected in the latter lesions in high frequencies (≥ 30%). Monoclonality was revealed in 21 (40%) of the 52 microdissected NAH, but was not found in any of the f ive ordinary nodules. LOH was found in all of the 13 NAH tested, being highly frequent at six loci on 8p, 11p, 13q and 17p. CONCLUSION: FNH, as a whole, is polyclonal, but some of the NAH lesions derived from it are already neoplastic and harbor similar allelic imbalances as HCAs.

Liver cell adenoma:A case report with clonal analysis and literature review

我们向它的 clonality 地位，病原的因素和鉴别诊断与特殊尊重在一个 33 岁的女病人汇报肝房间腺瘤(LCA ) 的一个案例。案例被组织病理学说，免疫组织化学和 clonality 试金在雄激素受体焦点在女体的纸巾和多型性在激活马赛克主义基于 X-chromosomal 检验。从中国和另外的国家的报导案例的 clinicopathological 特征被比较。损害是球形的，缩放在它的最大的尺寸的 2 厘米。组织学地，它由在绳索，其大多数是 two-cell-thick 并且由窦状隙分开了安排的房间组成。焦点的丰满的变化和过多的糖原贮积被观察。肿瘤房间在形状圆或多角形，类似于包围实质的房间。有丝分裂没被发现。没有门道，中央静脉或小导管在损害以内被发现。肿瘤组织为 cytokeratin (CK ) 显示出阳性反应 18，然而并非为 CK19， vimentin，雌激素和孕酮受体。Monoclonality 为损害被表明，证实 LCA 的诊断。Clonality 分析对它从焦点的榴状的增生的区别有用。

Primary malignant melanoma of the liver: A case report

Primary malignant melanoma of the liver is an exceedingly rare tumor. Only 12 cases have been reported in the worldwide literature. We present a case of isolated malignant melanoma of the liver occurring in a 36-year-old Chinese male patient. Comprehensive dermatologic and ophthalmologic examinations revealed no evidence of a cutaneous or ocular primary lesion. Other lesions in brain, respiratory tract, lung, gastrointestinal tract and anus, were not demonstrated by serial position emission tomography (PET). Microscopic examination of the resected specimen revealed a malignant melanoma, which was confi rmed by immunohistochemical staining for HMB-45, S-100 protein, melanoma-pan and vimentin. Moreover, electron microscopy demonstrated melanosomes in tumor cell cytoplasm. Our case shows that primary malignant melanoma may occur in the liver and should be considered when the histopathological appearance is not typical for other hepatic neoplasm.

Comparative mitochondrial genome analysis of Varunidae and its phylogenetic implications

Complete mitochondrial genomes(mitogenomes)can indicate phylogenetic relationships,as well as useful information for gene rearrangement mechanisms and molecular evolution.Currently,the phylogenetic location of the genus Varuna(Brachyura:Varunidae)has not been well resolved mainly because of limited representatives(only two extant species).Here,we determined a new mitogenome of this genus(Varuna litterata)and added the published mitogenomes to reconstruct the phylogeny of Varunidae.The 16368-bp mitogenome contains the entire set of 37 genes and a putative control region.The characteristics of this newly sequenced mitogenome were described and compared with the other 15 Varunidae mitogenomes.All 16 analyzed mitogenomes have identical gene order and similar molecular features.The sliding window and genetic distance analyses demonstrate highly variable nucleotide diversity,with comparatively low variability of COI and COII,and high variability of ND6.The nonsynonymous/synonymous substitution rates(dN/dS ratio)analysis shows that all 13 PCGs are under purifying selection and ATP8 gene evolves under the least selective pressure.Twelve tRNA genes,two rRNAs,one PCG,and the putative control region are found to be rearranged with respect to the pancrustacean ground pattern gene order.Tandem duplication/random loss model is adopted to explain the large-scale gene rearrangement events occurring in Varunidae mitogenomes.Phylogenetic analyses show that all Varunidae species are placed into one group,and form a sister clade with Macrophthalmidae.Nevertheless,the phylogenetic relationships within Varunidae are not completely consistent based on the two different datasets used in this study.These findings will contribute to a better understanding of gene rearrangement and molecular evolution in Varunidae mitogenomes,as well as provide insights into the phylogenetic studies of Brachyura.

Comparative mitochondrial genome analysis of Sesarmidae and its phylogenetic implications

Here,we sequenced the complete mitogenome of Parasesarma eumolpe(Brachyura:Grapsoidea:Sesarmidae)for the first time.The characteristics of this newly sequenced mitogenome were described and compared with other Sesarmidae species.The 15646-bp mitogenome contains 13 protein-coding genes(PCGs),two ribosomal RNA genes(r RNAs),22 transfer RNA genes(t RNAs),and an A-T rich region.All of the PCGs are initiated by the start codon ATN and terminated by the standard TAN codon or an incomplete T.The pairwise Ka/Ks ratio analysis shows that all 13 PCGs are under purifying selection,whereas the ATP8 gene is an outlier,with pairwise comparison values ranging from neutral selection(0.000)to positive selection(1.039).The gene arrangement of P.eumolpe compared with ancestral Decapoda shows the translocation of two t RNAs(t RNA-His and t RNA-Gln),which is identical to other Sesarmidae species.Phylogenetic analyses show that all Sesarmidae species are placed into one group,and the polyphyly of Eriphioidea,Ocypodoidea,and Grapsoidea is well supported.The relationship between gaps in the QIM region and the phylogeny of Sesarmidae is analyzed.It is obvious that both the G5(the gap between Q and I)and G6(the gap between I and M)decrease progressively with the evolution process.These results will help to better understand the genomic evolution within Sesarmidae and provide insights into the phylogeny of Brachyura.

The mechanism of slope instability due to rainfall-induced structural decay of earthquake-damaged loess

Natural loess slopes are characterized by a strong geological structure,which is an important factor in maintaining slope stability.The magnitude and duration of the earthquake may disturb the soil structure at different levels degrees,locally changing the arrangement between soil particles.The process of rainfall humidification weakens the cementation between soil particles,and the disturbance and humidification change the structural state of the soil,which in turn causes sliding of the slope along with the decay of soil mechanical properties.As slope instability is often the result of a series of post-earthquake ripple effects,it is of great scientific significance to study the mechanism of slope instability due to the structural decay of earthquake-damaged loess exacerbated by rainfall.In this paper,the impact of structural decay of loess on slope stability is simulated by GEOSTUDIO software under three conditions:pre-earthquake rainfall,post-earthquake rainfall and earthquake,taking the landslide in Buzi Village,Min County,Gansu Province as an example.The comparative analysis of the calculation results shows that the structural properties of the slope without earthquake disturbance are influenced by infil-tration amount.When it is fully saturated,the structural properties are similar to those of saturated soil,and the safety factor is reduced by 12.9%.In addition,the earthquake intensity and duration have different degrees of structural damage to the soil.When the structure is fully damaged,it is similar to that of remodelled soil,and the safety factor is reduced by 45.84%.Notably,the process of the earthquake and the following humidification generates the most serious damage to the loess structure,with a reduction in the safety factor of up to 56.15%.The quantitative analysis above obviously illustrates that the post-earthquake rainfall causes the most severe damage to structural loess slopes,and the resulting landslide hazard should not be underestimated.

全膝关节置换与单髁膝关节置换治疗膝关节骨关节炎术后患者皮肤感觉的变化

目的比较全膝关节置换术(TKA)与单髁膝关节置换术(UKA)治疗膝关节骨关节炎术后患者皮肤感觉的变化。方法选取2020年3月至2022年3月于本院拟行膝关节置换术治疗的62例膝关节骨关节炎患者(68个膝关节)作为研究对象,根据骨关节炎累及膝关节间室的不同分为TKA组(n=44)和UKA组(n=18)。TKA组行TKA治疗,UKA组行UKA治疗,比较两组切口周围皮肤感觉分级、术后困扰评分情况及术后困扰评分、切口周围皮肤感觉改变面积、切口长度,分析两组切口周围皮肤感觉改变面积、切口长度与术后困扰评分的相关性。结果两组切口周围皮肤感觉分级、术后困扰评分比较差异均无统计学意义。TKA组切口长度明显长于UKA组,切口周围皮肤感觉改变面积明显大于UKA组,差异有统计学意义(P<0.05)。TKA组切口周围皮肤感觉改变面积与术后困扰评分无相关性;UAK组切口周围皮肤感觉改变面积与术后困扰评分呈负相关(r<0,P<0.05),UKA组切口长度与术后困扰评分无相关性。结论延长切口长度会导致两种术式切口周围皮肤感觉面积发生改变,但TKA术后感觉影响较小,而UKA术后更易因损伤局部神经引起患者不适。

Huayu capsule enhances limb-catching capability of rats with experimental open traumatic brain injury

BACKGROUND:It is hard to cure the open traumatic brain injury(TBI),especially for the brain functional recovery after brain injury.In this regard,traditional Chinese medicine(TCM)has a wide prospect.OBJECTIVE:To observe the effect of Huayu capsule on limb-catching capability of rat models of open TBI,and investigate its possible mechanism.DESIGN:Randomized and controlled study.SETTING:Grade 3 Pharmacological Laboratory of TCM,State Administration of TCM,Chengdu University of TCM.MATERIALS:This study was performed from October 2005 to January 2006.Fifty Sprague-Dawley rats of either gender,aged 3 months old,weighing from 190 to 220 g,were involved in this study.Huayu capsule was made and supplied by the Department of TCM Processing of Chengdu University of TCM,Lot No.050121;Xuefuzhuyu oral liquid was manufactured by Jilin Aodong Yanbian Pharmaceutical Industry Co.,Ltd.,Lot No.050406.METHODS:Open right parietal lobe TBI rat models were made as described in references.The involved rat models were randomized into 5 groups according to gender and body mass:model group,high-,middle-,low-dose Huayu capsule groups and Xuefuzhuyu oral liquid group,with 10 rats in each.Rats in the model group were administrated with distilled water of 5 mL/kg;Rats in the high-,middle-and low-dose Huayu capsule groups were administrated with 1.030,0.515,0.258 g/kg raw herbs;Rats in the Xuefuzhuyu oral liquid group were administrated with Xuefuzhuyu oral liquid of 5 mL/kg,intragastrically once a day for 7 days successively for all after recovering consciousness from anesthetization.① One hour after administration on the 6th day,rats in each group were placed on a 100 cm fine straight iron wire paralleling to the ground and 20 cm above the operational table.The time of the rats keeping on the wire was counted and it indicated the nerve-muscle catching capability.The longer the remained time,the better the nerve-muscle catching capability.② Twenty-four hours after the administration on the 7th day,the samples of the whole brain were carefully taken out and stained by toluidine blue for observing the morphology of cells in the injured brain tissue.③ The nerve cells in 4 visual fields from 4 directions(upper,lower,left,right)of injured area of brain tissue were counted.The amount was the total number of the four visual fields.The nerve cells in the injured brain tissue were measured by the same way.④ On the basis of nerve cell counting,the content of Nissl's body in the corresponding nerve cells was measured.MAIN OUTCOME MEASURES:①Nerve-muscle catching capability.② Histopathomorphological examination of the injured areas in brain.③Measurement of nerve cells and macrophages in the injured areas of brain.④Measurement of content of Nissl's body in the injured areas of brain.RESULTS:All the 50 rats were involved in the final analysis.①The catching time of rats in the high-and middle-dose Huayu capsule groups as well as Xuefuzhuyu oral liquid group was extended to(23.6±10.12),(18.6±8.17)and(22.6±9.43)s,respectively,which was significantly higher than that in the model group(12.1±4.15)s,P < 0.05-0.01.② The injured areas of brain tissue of rats in the Huayu capsule-treated groups and Xuefuzhuyu oral liquid group were decreased to different extents.The nerve cells adjacent to brain injured area were increased.③ The number of macrophages around the brain injured area of rats in the high-and middle-dose Huayu capsule groups as well as Xuefuzhuyu oral liquid group was 63.9±7.99,59.7±7.41 and 62.9±7.37,respectively,which was significantly larger than that in the model group(49.2± 8.00,P < 0.01).The number of nerve cells adjacent to brain injured area of rats in the high-,middle-,and low-dose Huayu capsule groups as well as Xuefuzhuyu oral liquid group was 86.2±25.93,93.5±31.79,92.1±14.54 and 125.2±34.25,respectively,which was significantly larger than that in the model group(62.5±16.98,P < 0.05-0.01).④ The total area,the total and integral absorbance,the average graydegree of Nissl's body in the cytoplasm of nerve cells of rats in the Huayu capsule-treated groups and Xuefuzhuyu oral liquid group were all significantly increased(P < 0.05-0.01).CONCLUSION:Huayu capsule at different doses can promote the limb-catching capability of rat models of open TBI to different extent.This promoting effect may be related to increasing macrophages in the injured area,lessening the apoptosis of nerve cells and increasing the content of Nissl's body in the nerve cells.

Formation and Compression Behavior of Two-Phase Bulk Metallic Glasses with a Minor Addition of Aluminum

A remarkable enhancement in room-temperature compressive deformability is realized by the minor-addition of 1.5 at.%Al in ZrTi-based bulk metallic glass.Two amorphous phases are observed by transmission electron microscopy in the Al-containing alloys and this explains the improvement of compression deformability.The studies suggest that phase separation might occur in glass forming alloys with a negative enthalpy of mixing.

Structural Relaxation of Pd_(39)Ni_(10)Cu_(30)P_(21) Bulk Metallic Glass Under High Pressure

The Pd_(39)Ni_(10)Cu_(30)P_(21) bulk metallic glass is isothermally relaxed under various pressures.The degree of the structural relaxation is evaluated in terms of the enthalpy recovery behaviours involved in the irreversible glass transition processes by using a temperature-modulated differential scanning calarimetry technique.A roughly linear increase of the recovery enthalpy is observed within the experimental pressure range from 2.67 to 4.45 GPa,which reflects the release of the frozen-in enthalpy in the as-quenched glass with increasing relaxation pressure.The pressure dependence of the time scale of the enthalpy recovery processes is also exhibited.

一种用于目标跟踪边界框回归的光滑IoU损失

边界框回归分支是深度目标跟踪器的关键模块,其性能直接影响跟踪器的精度.评价精度的指标之一是交并比(Intersection over union,IoU).基于IoU的损失函数取代了l_(n)-norm损失成为目前主流的边界框回归损失函数,然而IoU损失函数存在2个固有缺陷:1)当预测框与真值框不相交时IoU为常量0,无法梯度下降更新边界框的参数;2)在IoU取得最优值时其梯度不存在,边界框很难收敛到IoU最优处.揭示了在回归过程中IoU最优的边界框各参数之间蕴含的定量关系,指出在边界框中心处于特定位置时存在多种尺寸不同的边界框使IoU损失最优的情况,这增加了边界框尺寸回归的不确定性.从优化两个统计分布之间散度的视角看待边界框回归问题,提出了光滑IoU(Smooth-IoU,SIoU)损失,即构造了在全局上光滑(即连续可微)且极值唯一的损失函数,该损失函数自然蕴含边界框各参数之间特定的最优关系,其唯一取极值的边界框可使IoU达到最优.光滑性确保了在全局上梯度存在使得边界框更容易回归到极值处,而极值唯一确保了在全局上可梯度下降更新参数,从而避开了IoU损失的固有缺陷.提出的光滑损失可以很容易取代IoU损失集成到现有的深度目标跟踪器上训练边界框回归,在LaSOT、GOT-10k、TrackingNet、OTB2015和VOT2018测试基准上所取得的结果,验证了光滑IoU损失的易用性和有效性.

Effect of Huangqi Xiaoke Recipe combined with alprostadil on qi and yin deficiency type diabetic nephropathy and its effect on vascular endothelium and oxidative stress

Objective: To observe the clinical efficacy of Huangqi Xiaoke Decoction combined with alprostadil on qi and yin deficiency type diabetic nephropathy (DN) and its effect on vascular endothelium and oxidative stress. Methods: A total of 72 patients with qi and yin deficiency type DN who were admitted to the diabetes specialist ward and outpatient department of Baoan District Hospital of Shenzhen from January 2017 to July 2018 were enrolled. The patients were divided into the control group and the observation group according to the random number method. 36 cases in each group. Both groups were treated with conventional treatments such as hypoglycemic, hypotensive, lipid-lowering and anti-platelet aggregation. The control group was treated with alprostadil on the basis of conventional treatment. The observation group was given orally on the basis of the control group. Both groups were treated once a day for a total of 4 weeks. Compare the clinical syndrome scores and clinical efficacy of the two groups before and after treatment;fasting blood glucose (FPG), glycosylated hemoglobin (HbA1C), blood lipids (TC, TG, LDL, HDL), serum creatinine (SCr), blood urea nitrogen (BUN Endothelin-1 (ET-1), nitric oxide (NO), von Willebrand factor (vWF);glutathione peroxidase (GSH-Px), superoxide disproportionation The level of enzyme (SOD), malondialdehyde (MDA). Results:The total effective rate of TCM syndromes in observation group was 94.44%, which was significantly different from 72.22% in the control group. The scores of TCM syndromes in the observation group were significantly lower than those in the control group;Compared with the control group, the HDL-C of the observation group did not change much, there was no statistical difference;FBG, HbA1C, SCr, BUN, TC, TG, LDL-C, ET-1, vWF, MDA Significantly decreased, NO, GSH-Px, SOD increased significantly, with significant difference. Conclusion: Huangqi Xiaoke Decoction combined with alprostadil in the treatment of qi and yin deficiency type DN has significant curative effect, which can not only lower blood sugar, regulate blood lipids, improve renal function and clinical symptoms, but also inhibit oxidative stress and protect endothelial function.

Research progress in pharmacological and clinical application of Jingui Shenqi pill

Objective:To provide ideas for more scientific and rational application of Jingui Shenqi pill in the treatment of diseases.Methods:We searched and analyzed the literatures about the composition principle,pharmacology and clinical researches of Jingui Shenqi pill.Conclusion:The pharmacology mechanism researches about Jingui Shenqi pill were extensive and in-depth.Combining the pharmacology with the clinical research should become a new direction of Jingui Shenqi pill,and can also provide reference for clinical use of this prescription.

基于DRL的巨型星座星地测控链路规划算法

针对巨型星座的星地测控链路规划问题,提出了一种基于深度强化学习的智能规划调度算法。该方法考虑了卫星对于测控站的资源竞争关系和连接关系,设计了环境状态,决策智能体通过感知卫星状态,结合动作选择策略,生成卫星对于测控站的分配方案,并根据反馈的奖励值进行策略的迭代优化。将本算法应用于巨型星座系统的星地测控链路规划任务,仿真结果表明所提出的智能算法可以将测控站天线利用率提升到98%以上,同时有效地降低了天线的切换次数。另外,训练好的模型可以根据未来时刻的星地可视窗口,在30s内快速生成星地测控链路规划方案。

调节性T细胞联合血清外泌体ZFPL1在非小细胞肺癌诊断中的临床意义

目的探讨调节性T细胞(Treg)联合血清外泌体ZFPL1在非小细胞肺癌诊断中的价值。方法收集2022年1月至2022年10月收治的非小细胞肺癌患者67例为观察组,选择同期健康体检者61例为对照组。采用流式细胞仪测定Treg细胞标志物CD3^(+)CD8^(+)和CD4^(+)Foxp3^(+)水平;采用实时荧光PCR(qPCR)测定血清外泌体ZFPL1水平,分析Treg及血清外泌体ZFPL1表达水平与临床病理特征的关系;绘制受试者特征工作曲线(ROC)评价Treg联合血清外泌体ZFPL1诊断非小细胞肺癌的效能。结果观察组Treg细胞标志物CD3^(+)CD8^(+)水平为0.48±0.11,低于对照组的0.59±0.16(P<0.05);观察组血清外泌体ZFPL1水平和Treg细胞标志物CD4^(+)Foxp3^(+)水平分别为3.62(1.44,7.62)和0.76±0.06,高于对照组的0.91(0.17,3.43)和0.67±0.19,差异均有统计学意义(P<0.05)。CD4^(+)Foxp3^(+)及血清外泌体ZFPL1水平与分期和病理类型有关(P<0.05)。Treg细胞标志物CD3^(+)CD8^(+)、CD4^(+)Foxp3^(+)及血清外泌体ZFPL1联合检测诊断非小细胞肺癌患者的效能高于单一指标(P<0.05)。结论Treg细胞标志物CD4^(+)Foxp3^(+)和血清外泌体ZFPL1在非小细胞肺癌中呈高表达,CD3^(+)CD8^(+)水平呈低表达,能准确地实现术前分期,不同指标联合测定能提高诊断效能。

复配添加剂对石蜡物理性能影响的研究

沿用《几种常见添加剂对石蜡物理性能影响的研究》一文的研究方法和结论,选取6种性质较为突出的添加剂进行两两组合,设计出3组复配添加剂对石蜡物理性能影响的研究试验。根据试验结果绘制数学模型图,并根据数学模型图颜色深浅、疏密度等因素,判断其对石蜡物理性能的影响趋势,从而得出复配添加剂对石蜡物理性能影响的结论:复配添加剂对石蜡的改性基本符合各单剂的改性规律特点,同时它们之间又发生相互作用,既有协同效应,也有对抗效应,并不是单纯加和而已。根据以上规律,可以对各添加剂加以巧妙利用,进行优化组合、搭配,根据生产的实际需求选取适合的复配添加剂,以满足石蜡、特种蜡产品生产的特殊要求。