目的探讨绝经后女性血常规指标与骨髓脂肪含量、骨密度相关性。方法选取72名绝经后女性(年龄55~79岁),测量受试者身高、体重、红细胞计数、白细胞计数、血小板计数,计算体质量指数(body mass index,BMI),运用磁共振波谱成像(magnetic re...目的探讨绝经后女性血常规指标与骨髓脂肪含量、骨密度相关性。方法选取72名绝经后女性(年龄55~79岁),测量受试者身高、体重、红细胞计数、白细胞计数、血小板计数,计算体质量指数(body mass index,BMI),运用磁共振波谱成像(magnetic resonance spectroscopy,MRS)扫描L_(3)椎体骨髓脂肪含量(fat fraction,FF),通过双能X线吸收检测(dual-energy X-ray absorptiometry,DXA)扫描获取受试者腰椎(L_(1~4))、左股骨颈及全身骨密度(bone mineral density,BMD)值。评价MRS扫描可重复性,分析红细胞计数、白细胞计数、血小板计数与FF值及BMD值相关性,运用多元逐步回归分析FF值的独立相关因素。结果L_(3)椎体FF值测量可重复性变异系数(CV)为2.86%。红细胞计数、白细胞计数、血小板计数与FF呈负相关(P<0.01);红细胞计数、白细胞计数、血小板计数与腰椎(L_(1~4))骨密度、左股骨颈骨密度无相关性,与全身骨密度呈负相关(P<0.05)。进一步多元逐步回归分析结果表明:校正了年龄、身高、体重、BMI后,红细胞计数、白细胞计数、血小板计数是影响FF的独立负性相关因素(P<0.05,R^(2)=0.518)。结论血常规指标(红细胞计数、白细胞计数、血小板计数)与FF及绝经后女性骨密度有一定的相关性,对绝经后女性骨质疏松症的早期诊断与防治有一定指导作用。展开更多
Objective： To explore the regulating effects of cefodizime on cytokines expression of neutrophil response to Klebsiella pneumoniae （Kle. p） treatment. Methods： We detected the types and expression of cytokines sec...Objective： To explore the regulating effects of cefodizime on cytokines expression of neutrophil response to Klebsiella pneumoniae （Kle. p） treatment. Methods： We detected the types and expression of cytokines secreted by neutrophils by cDNA array and RT-PCR. We also analyzed the changes of signal transduction in this process by detecting the expression of toll like receptor 4 （TLR4） and the inhibitor factor of κBα （I-κBα） expressed by neutrophils. The activity of NF-κB DNA-binding in neutrophils was measured by electrophoretic mobility shift assay （EMSA）. Results： Cefodizime increased the neutrophils production of TNF-α, IL-β3 and the mRNA expression of TLR4 in the early stage of Kle. p stimulation in mice, which seemed corresponding to the enhanced NF-κB DNA-binding activity. Conclusion： Cefodizime regulates the cytokines expression of neutrophils through the LPS-TLR4-NF-κB pathway by affecting the expression of TLR4 mRNA and the DNA binding activities of NF-κB in mice with the challenge of Kle. p.展开更多
文摘目的探讨绝经后女性血常规指标与骨髓脂肪含量、骨密度相关性。方法选取72名绝经后女性(年龄55~79岁),测量受试者身高、体重、红细胞计数、白细胞计数、血小板计数,计算体质量指数(body mass index,BMI),运用磁共振波谱成像(magnetic resonance spectroscopy,MRS)扫描L_(3)椎体骨髓脂肪含量(fat fraction,FF),通过双能X线吸收检测(dual-energy X-ray absorptiometry,DXA)扫描获取受试者腰椎(L_(1~4))、左股骨颈及全身骨密度(bone mineral density,BMD)值。评价MRS扫描可重复性,分析红细胞计数、白细胞计数、血小板计数与FF值及BMD值相关性,运用多元逐步回归分析FF值的独立相关因素。结果L_(3)椎体FF值测量可重复性变异系数(CV)为2.86%。红细胞计数、白细胞计数、血小板计数与FF呈负相关(P<0.01);红细胞计数、白细胞计数、血小板计数与腰椎(L_(1~4))骨密度、左股骨颈骨密度无相关性,与全身骨密度呈负相关(P<0.05)。进一步多元逐步回归分析结果表明:校正了年龄、身高、体重、BMI后,红细胞计数、白细胞计数、血小板计数是影响FF的独立负性相关因素(P<0.05,R^(2)=0.518)。结论血常规指标(红细胞计数、白细胞计数、血小板计数)与FF及绝经后女性骨密度有一定的相关性,对绝经后女性骨质疏松症的早期诊断与防治有一定指导作用。
基金the Natural Science Foundation of GuangdongProvince (5300983)
文摘Objective： To explore the regulating effects of cefodizime on cytokines expression of neutrophil response to Klebsiella pneumoniae （Kle. p） treatment. Methods： We detected the types and expression of cytokines secreted by neutrophils by cDNA array and RT-PCR. We also analyzed the changes of signal transduction in this process by detecting the expression of toll like receptor 4 （TLR4） and the inhibitor factor of κBα （I-κBα） expressed by neutrophils. The activity of NF-κB DNA-binding in neutrophils was measured by electrophoretic mobility shift assay （EMSA）. Results： Cefodizime increased the neutrophils production of TNF-α, IL-β3 and the mRNA expression of TLR4 in the early stage of Kle. p stimulation in mice, which seemed corresponding to the enhanced NF-κB DNA-binding activity. Conclusion： Cefodizime regulates the cytokines expression of neutrophils through the LPS-TLR4-NF-κB pathway by affecting the expression of TLR4 mRNA and the DNA binding activities of NF-κB in mice with the challenge of Kle. p.
