Using homology cloning method, a heat shock transcription factor （Hsf） like gene, ZmHsf-like, was cloned from maize （Zea mays） leaves. Sequence analyses showed that the open reading frame （ORF） of the gene ZmHsf...Using homology cloning method, a heat shock transcription factor （Hsf） like gene, ZmHsf-like, was cloned from maize （Zea mays） leaves. Sequence analyses showed that the open reading frame （ORF） of the gene ZmHsf-like is 1 404 bp long, encoding 467 amino acids. The sequence of amino acids encoded by ZmHsf-like contains the most conserved and typical DNA-binding domain of Hsf family. By bombardment into onion epidermis, we ifrstly found that the ZmHsf-like was subcellular-located in nucleus. NucPred analysis revealed there is a classic NLS of KKRR peptide in protein. Real-time PCR showed that ZmHsf-like gene expressed in leaves, stems and roots of maize seedlings under normal growth conditions, and the highest expression level was in roots, lower in leaves and the lowest in stems. The ZmHsf-like gene expression could be up-regulated by heat shock, PEG, ABA, and H2O2 in different degrees, among which the heat shock and ABA worked more efifciently. Obvious differences of the peak value and its corresponding time point of ZmHsf-like gene expression were observed among treatments. Experiments with inhibitor further suggested that the up-regulation ZmHsf-like gene expression of heat shock was H2O2-dependent while the induction of ZmHsf-like with PEG did not depend on the existence of H2O2. These results pointed out that ZmHsf-like gene probably regulates responsive reactions to abiotic stresses especially heat shock and drought through different signal transduction pathways.展开更多
中国巡天空间望远镜(China Space Survey Telescope,CSST)是中国载人航天工程规划建设的以大规模天文巡天为主任务的2m口径空间光学望远镜,兼具高空间分辨率与大视场特性,性能优异。暂现源是CSST的重要科学目标之一.CSST作为空间望远镜...中国巡天空间望远镜(China Space Survey Telescope,CSST)是中国载人航天工程规划建设的以大规模天文巡天为主任务的2m口径空间光学望远镜,兼具高空间分辨率与大视场特性,性能优异。暂现源是CSST的重要科学目标之一.CSST作为空间望远镜,与地面望远镜的图像噪声组成有着明显不同。因此,不能直接套用先前地基巡天项目的暂现源探测方案,需要针对性开发专属的探测方法.CSST的暂现源探测方法以CSST仿真数据为基础进行开发。验证,旨在尽可能准确地识别CSST主巡天数据中的暂现源,方法以图像相减法为基础,首先获得观测图像所对应残差图像,并利用滤波方法给出残差图像泊松噪声分布;然后使用渐变阈值间接统一背景噪声水平,对残差图像进行目标源测光得到候选源列表;最后,通过判断候选源与当地泊松噪声的偏差对真实暂现源进行筛选,结合原始图像信息与观测图像测光分类信息后,进一步对候选源列表的假源进行剔除,最终输出暂现源星表。方法在共计20000个暂现源的多轮仿真测试中,能够以平均95.9%的准确率对观测图像中的暂现源进行筛选,相较于先前工作,还更加全面且定量地给出了测试所得的不同亮度暂现源所对应的探测率,测试结论验证了CSST暂现源探测方法的可行性、泛化能力与稳定性。该方法也为空间望远镜暂现源探测任务提供了理论与编程基础.展开更多
基金supported by grants from the Key Project of Application Basic Research of Hebei Province,China (12965517D)the Youth Fund of Hebei Academy of Agriculture and Forestry Sciences,China (A09110103).
文摘Using homology cloning method, a heat shock transcription factor （Hsf） like gene, ZmHsf-like, was cloned from maize （Zea mays） leaves. Sequence analyses showed that the open reading frame （ORF） of the gene ZmHsf-like is 1 404 bp long, encoding 467 amino acids. The sequence of amino acids encoded by ZmHsf-like contains the most conserved and typical DNA-binding domain of Hsf family. By bombardment into onion epidermis, we ifrstly found that the ZmHsf-like was subcellular-located in nucleus. NucPred analysis revealed there is a classic NLS of KKRR peptide in protein. Real-time PCR showed that ZmHsf-like gene expressed in leaves, stems and roots of maize seedlings under normal growth conditions, and the highest expression level was in roots, lower in leaves and the lowest in stems. The ZmHsf-like gene expression could be up-regulated by heat shock, PEG, ABA, and H2O2 in different degrees, among which the heat shock and ABA worked more efifciently. Obvious differences of the peak value and its corresponding time point of ZmHsf-like gene expression were observed among treatments. Experiments with inhibitor further suggested that the up-regulation ZmHsf-like gene expression of heat shock was H2O2-dependent while the induction of ZmHsf-like with PEG did not depend on the existence of H2O2. These results pointed out that ZmHsf-like gene probably regulates responsive reactions to abiotic stresses especially heat shock and drought through different signal transduction pathways.
文摘中国巡天空间望远镜(China Space Survey Telescope,CSST)是中国载人航天工程规划建设的以大规模天文巡天为主任务的2m口径空间光学望远镜,兼具高空间分辨率与大视场特性,性能优异。暂现源是CSST的重要科学目标之一.CSST作为空间望远镜,与地面望远镜的图像噪声组成有着明显不同。因此,不能直接套用先前地基巡天项目的暂现源探测方案,需要针对性开发专属的探测方法.CSST的暂现源探测方法以CSST仿真数据为基础进行开发。验证,旨在尽可能准确地识别CSST主巡天数据中的暂现源,方法以图像相减法为基础,首先获得观测图像所对应残差图像,并利用滤波方法给出残差图像泊松噪声分布;然后使用渐变阈值间接统一背景噪声水平,对残差图像进行目标源测光得到候选源列表;最后,通过判断候选源与当地泊松噪声的偏差对真实暂现源进行筛选,结合原始图像信息与观测图像测光分类信息后,进一步对候选源列表的假源进行剔除,最终输出暂现源星表。方法在共计20000个暂现源的多轮仿真测试中,能够以平均95.9%的准确率对观测图像中的暂现源进行筛选,相较于先前工作,还更加全面且定量地给出了测试所得的不同亮度暂现源所对应的探测率,测试结论验证了CSST暂现源探测方法的可行性、泛化能力与稳定性。该方法也为空间望远镜暂现源探测任务提供了理论与编程基础.
