Objective: This study was performed to evaluate whether implantation of mesenchymal stem cell (MSC) would reduce left ventricular remodelling from the molecular mechanisms compared with angiotensin-converting enzyme i...Objective: This study was performed to evaluate whether implantation of mesenchymal stem cell (MSC) would reduce left ventricular remodelling from the molecular mechanisms compared with angiotensin-converting enzyme inhibitors (ACEIs) perindopril into ischemic myocardium after acute myocardial infarction. Methods: Forty rats were divided into four groups: control, MSC, ACEI, MSC+ACEI groups. Bone marrow stem cell derived rat was injected immediately into a zone made ischemic by coronary artery ligation in MSC group and MSC+ACEI group. Phosphate-buffered saline (PBS) was injected into control group. Perindopril was administered p.o. to ACEI group and MSC+ACEI group. Six weeks after implantation, the rats were killed and heart sample was collected. Fibrillar collagen was observed by meliorative Masson’s trichome stain. Western Blotting was employed to evaluate the protein expression of matrix metalloproteinase (MMP)-2, matrix metalloproteinase (MMP)-9 in infarction zone. The transcriptional level of MMP2, MMP9 and tissue inhibitor of matrix metalloproteinase (TIMP)-1 in infarction area was detected by reverse transcriptase PCR (RT-PCR) analysis. Results: The fibrillar collagen area, the protein expression of MMP2, MMP9 and the transcriptional level of MMP2, MMP9 mRNA in infarction zone reduced in MSC group, ACEI group, and MSC+ACEI group. No significant difference was detected in the expression of TIMP1 mRNA among the 4 groups. Conclusion: Both MSC and ACEI could reduce infarction remodelling by altering collagen metabolism.展开更多
The number of intensive livestock and poultry farms is expected to increase substantially in future because of consumer demand.Unfortunately,such demand also results in a great deal of manure being generated,which thr...The number of intensive livestock and poultry farms is expected to increase substantially in future because of consumer demand.Unfortunately,such demand also results in a great deal of manure being generated,which threatens the environment if it is not properly managed.Concurrent developments in biotransformation of these wastes with the black soldier fly(BSF),Hermetia illucens(L.)(Diptera:Stratiomydiae),demonstrates such concerns can be abated,while also producing products(e.g.,protein,chitin,biodiesel,and fertilizer)of value.In this review,we analyzed the factors influencing black soldier fly larvae(BSFL)conversion processes,the role of microorganisms,and the mechanisms used by BSFL when converting livestock and poultry manure into these valuable products.The effects of BSFL conversion technology on reducing the hazards of such materials and their associated pathogens are reviewed,and the economics of livestock and poultry manure conversion by BSF larvae is discussed.展开更多
目的探讨耳内镜下分离前下皮瓣修补鼓膜前下象限边缘性穿孔的临床疗效和应用价值。方法对资料完整的13例鼓膜前下象限边缘性穿孔患者的临床资料进行回顾性分析。对比患者手术前后纯音听阈和耳内镜结果,计算气骨导差及气骨导差改善值。...目的探讨耳内镜下分离前下皮瓣修补鼓膜前下象限边缘性穿孔的临床疗效和应用价值。方法对资料完整的13例鼓膜前下象限边缘性穿孔患者的临床资料进行回顾性分析。对比患者手术前后纯音听阈和耳内镜结果,计算气骨导差及气骨导差改善值。结果术后3个月复查,耳内镜下所有患者鼓膜完整。平均气导为(16. 0±15. 64) d B,骨导为(12. 16±11. 92) d B,气骨导差为(3. 83±3. 73) d B,术后3个月的气骨导差明显小于术前(P <0. 05)。结论耳内镜下分离前下皮瓣修补鼓膜前下象限穿孔,具有简单,微创,愈合率高的特点,值得临床推广。展开更多
文摘Objective: This study was performed to evaluate whether implantation of mesenchymal stem cell (MSC) would reduce left ventricular remodelling from the molecular mechanisms compared with angiotensin-converting enzyme inhibitors (ACEIs) perindopril into ischemic myocardium after acute myocardial infarction. Methods: Forty rats were divided into four groups: control, MSC, ACEI, MSC+ACEI groups. Bone marrow stem cell derived rat was injected immediately into a zone made ischemic by coronary artery ligation in MSC group and MSC+ACEI group. Phosphate-buffered saline (PBS) was injected into control group. Perindopril was administered p.o. to ACEI group and MSC+ACEI group. Six weeks after implantation, the rats were killed and heart sample was collected. Fibrillar collagen was observed by meliorative Masson’s trichome stain. Western Blotting was employed to evaluate the protein expression of matrix metalloproteinase (MMP)-2, matrix metalloproteinase (MMP)-9 in infarction zone. The transcriptional level of MMP2, MMP9 and tissue inhibitor of matrix metalloproteinase (TIMP)-1 in infarction area was detected by reverse transcriptase PCR (RT-PCR) analysis. Results: The fibrillar collagen area, the protein expression of MMP2, MMP9 and the transcriptional level of MMP2, MMP9 mRNA in infarction zone reduced in MSC group, ACEI group, and MSC+ACEI group. No significant difference was detected in the expression of TIMP1 mRNA among the 4 groups. Conclusion: Both MSC and ACEI could reduce infarction remodelling by altering collagen metabolism.
基金the National Key R&D Program of China(2018YFD0500203 and 2018YFF0213503)the National Natural Science Foundation of China(31770136)。
文摘The number of intensive livestock and poultry farms is expected to increase substantially in future because of consumer demand.Unfortunately,such demand also results in a great deal of manure being generated,which threatens the environment if it is not properly managed.Concurrent developments in biotransformation of these wastes with the black soldier fly(BSF),Hermetia illucens(L.)(Diptera:Stratiomydiae),demonstrates such concerns can be abated,while also producing products(e.g.,protein,chitin,biodiesel,and fertilizer)of value.In this review,we analyzed the factors influencing black soldier fly larvae(BSFL)conversion processes,the role of microorganisms,and the mechanisms used by BSFL when converting livestock and poultry manure into these valuable products.The effects of BSFL conversion technology on reducing the hazards of such materials and their associated pathogens are reviewed,and the economics of livestock and poultry manure conversion by BSF larvae is discussed.
文摘目的探讨耳内镜下分离前下皮瓣修补鼓膜前下象限边缘性穿孔的临床疗效和应用价值。方法对资料完整的13例鼓膜前下象限边缘性穿孔患者的临床资料进行回顾性分析。对比患者手术前后纯音听阈和耳内镜结果,计算气骨导差及气骨导差改善值。结果术后3个月复查,耳内镜下所有患者鼓膜完整。平均气导为(16. 0±15. 64) d B,骨导为(12. 16±11. 92) d B,气骨导差为(3. 83±3. 73) d B,术后3个月的气骨导差明显小于术前(P <0. 05)。结论耳内镜下分离前下皮瓣修补鼓膜前下象限穿孔,具有简单,微创,愈合率高的特点,值得临床推广。
