miR-140-5p调控Nrf2影响结肠癌细胞5-FU耐药性

目的观察miR-140-5p对结肠癌耐药细胞株SW480/5-FU及其亲本细胞株SW480 5-FU敏感性的影响及其与核因子NF-E2相关因子2(NF-E2-related factor 2,Nrf2)的关系,探讨miR-140-5p调控结肠癌细胞5-FU耐药的可能机制。方法用结肠癌细胞株SW480构建结肠癌耐药细胞株SW480/5-FU,qRT-PCR法检测两株细胞中miR-140-5p的表达,CCK-8法和细胞集落形成实验评估miR-140-5p对两株细胞5-FU敏感性的影响;采用qRT-PCR法、Western blot法双荧光素酶报告基因验证miR-140-5p与Nrf2的关系,CCK-8法、细胞集落形成实验探讨miR-140-5p调控结肠癌细胞5-FU敏感性与Nrf2的关系。结果成功构建结肠癌耐药细胞株SW480/5-FU,其miR-140-5p表达水平显著低于亲本细胞株SW480(P<0.05);CCK-8法、细胞集落形成实验结果显示,miR-140-5p可增强SW480/5-FU细胞对5-FU的敏感性,而降低SW480对5-FU的耐药性;qRT-PCR法、Westren blot法、双荧光素酶报告基因实验共同证实miR-140-5p可结合并抑制Nrf2的表达;CCK-8、细胞集落形成实验表明,Nrf2过表达能逆转miR-140-5p对结肠癌耐药细胞SW480/5-FU的5-FU敏感性调控结果。结论 miR-140-5p可能通过结合并抑制Nrf2表达,增加结肠癌耐药细胞株SW480/5-FU对5-FU的敏感性。

Surviving Flying Tigers and Their Enduring Spirit:an Unforgettable Meeting with Survivors

On the evening of September 4,on behalf of General Fang Fenghui,chief of the PLA General Staff,I met some members of the legendary Flying Tigers of World WarⅡ.An Exciting Conversation Decorative lanterns illuminated Chang*an Avenue,greeting a