Cell wall is the first physical barrier to aluminum(Al)toxicity.Modification of cell wall properties to change its binding capacity to Al is one of the major strategies for plant Al resistance;nevertheless,how it is r...Cell wall is the first physical barrier to aluminum(Al)toxicity.Modification of cell wall properties to change its binding capacity to Al is one of the major strategies for plant Al resistance;nevertheless,how it is regulated in rice remains largely unknown.In this study,we show that exogenous application of putrescines(Put)could significantly restore the Al resistance of art1,a rice mutant lacking the central regulator Al RESISTANCE TRANSCRIPTION FACTOR 1(ART1),and reduce its Al accumulation particularly in the cell wall of root tips.Based on RNA-sequencing,yeast-onehybrid and electrophoresis mobility shift assays,we identified an R2R3 MYB transcription factor OsMYB30 as the novel target in both ART1-dependent and Put-promoted Al resistance.Furthermore,transient dual-luciferase assay showed that ART1 directly inhibited the expression of OsMYB30,and in turn repressed Os4CL5-dependent 4-coumaric acid accumulation,hence reducing the Al-binding capacity of cell wall and enhancing Al resistance.Additionally,Put repressed OsMYB30 expression by eliminating Alinduced H2O2accumulation,while exogenous H2O2promoted OsMYB30 expression.We concluded that ART1 confers Put-promoted Al resistance via repression of OsMYB30-regulated modification of cell wall properties in rice.展开更多
PbO_2/activated carbon（AC） hybrid supercapacitor in H_2SO_4 with a carbon foam current collector is studied.The PbO_2/AC hybrid is designed with electrodeposited PbO_2 thin film as positive electrode to match with A...PbO_2/activated carbon（AC） hybrid supercapacitor in H_2SO_4 with a carbon foam current collector is studied.The PbO_2/AC hybrid is designed with electrodeposited PbO_2 thin film as positive electrode to match with AC negative electrode.The discharge curve shows capacitive characteristics between 1.88 V and 0.65 V.The hybrid system exhibits excellent energy and power performance,with specific energy of 43.6 Wh/kg at a power density of 654.2 W/kg.The use of carbon foam current collector ensures stability of the PbO_2 electrode in H_2SO_4 environment.After 2600 deep cycles at 15 C high rate of charge/discharge,the capacity remains nearly unchanged from its initial value.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.31210103907)Guangdong Laboratory for Lingnan Modern Agriculture(Grant No.NT2021010)+1 种基金Research Program for Ecological Civilization and Innovation of Environmental Science and Technology in Zhejiang University,111 Project(Grant No.B14027)Grantin-Aid for Specially Promoted Research(JSPS KAKENHI Grant No.21H05034 to J.F.M.)。
文摘Cell wall is the first physical barrier to aluminum(Al)toxicity.Modification of cell wall properties to change its binding capacity to Al is one of the major strategies for plant Al resistance;nevertheless,how it is regulated in rice remains largely unknown.In this study,we show that exogenous application of putrescines(Put)could significantly restore the Al resistance of art1,a rice mutant lacking the central regulator Al RESISTANCE TRANSCRIPTION FACTOR 1(ART1),and reduce its Al accumulation particularly in the cell wall of root tips.Based on RNA-sequencing,yeast-onehybrid and electrophoresis mobility shift assays,we identified an R2R3 MYB transcription factor OsMYB30 as the novel target in both ART1-dependent and Put-promoted Al resistance.Furthermore,transient dual-luciferase assay showed that ART1 directly inhibited the expression of OsMYB30,and in turn repressed Os4CL5-dependent 4-coumaric acid accumulation,hence reducing the Al-binding capacity of cell wall and enhancing Al resistance.Additionally,Put repressed OsMYB30 expression by eliminating Alinduced H2O2accumulation,while exogenous H2O2promoted OsMYB30 expression.We concluded that ART1 confers Put-promoted Al resistance via repression of OsMYB30-regulated modification of cell wall properties in rice.
基金the financial support of this research work from Natural Science Foundation of China(No 20663005)
文摘PbO_2/activated carbon（AC） hybrid supercapacitor in H_2SO_4 with a carbon foam current collector is studied.The PbO_2/AC hybrid is designed with electrodeposited PbO_2 thin film as positive electrode to match with AC negative electrode.The discharge curve shows capacitive characteristics between 1.88 V and 0.65 V.The hybrid system exhibits excellent energy and power performance,with specific energy of 43.6 Wh/kg at a power density of 654.2 W/kg.The use of carbon foam current collector ensures stability of the PbO_2 electrode in H_2SO_4 environment.After 2600 deep cycles at 15 C high rate of charge/discharge,the capacity remains nearly unchanged from its initial value.
