米格列醇+格列吡嗪治疗2型糖尿病患者的临床疗效及对血糖水平的影响评价

目的探讨米格列醇+格列吡嗪治疗2型糖尿病患者的临床疗效及对血糖水平的影响。方法选取2021年10月-2023年10月福建中医药大学附属人民医院收治的84例2型糖尿病的患者为研究对象,依据随机数表法分为两组,各42例。对照组给予格列吡嗪单药服,观察组给予米格列醇+格列吡嗪共治,对比两组临床疗效、血糖控制情况、胰岛功能以及用药安全性。结果观察组治疗总有效率(95.24%)高于对照组(80.95%),差异有统计学意义(χ^(2)=4.086,P=0.043)。用药后,观察组各项血糖控制指标、胰岛功能指标均低于对照组,差异有统计学意义(P均<0.05)。两组不良反应发生率比较,差异无统计学意义(P>0.05)。结论米格列醇+格列吡嗪治疗可明显提升2型糖尿病患者的用药疗效,更有效调节机体血糖及胰岛功能,同时保障用药安全性。

营养液浓度动态调控和液位高度对水培叶用莴苣生长及品质的影响

以半结球叶用莴苣品种富兰德里为试材,在深液流水培(DFT)模式下研究了营养液浓度动态调控和液位高度对叶用莴苣生长及品质的影响。结果表明:随着营养液浓度调控幅度的提高,叶用莴苣生长指标和可溶性蛋白、可溶性糖、VC等品质指标均呈先上升后下降的变化趋势,以每8 d提高0.36 mS · cm^(-1)处理的效果最好,叶片长、叶片宽、叶绿素a含量、总叶绿素含量、根系活力、地上部和地下部鲜质量及干质量、可溶性蛋白含量、可溶性糖含量、VC含量均显著高于其他处理,其中地上部鲜质量较其他处理提高了22.3%~57.9%;不同营养液液位高度对叶用莴苣生长和品质的影响存在显著差异,12~16 cm液位高度处理的植株生长和品质总体上优于其他处理,4 cm液位高度处理的效果较差。

设施蓝莓园区发展策略研究——以云南蒙自市3家园区为例

贯彻落实习近平总书记重要指示精神和党中央、国务院决策部署,加快现代设施农业发展,为设施农业园区发展带来了大好机遇与政策指引。本研究以云南蒙自市设施蓝莓园区为例,探索实践“基层党组织+农户+龙头企业+国有公司”多种利益联结与运营管理模式,典型发展模式有红河佳裕“兜底增收”模式、云科创“孵化家庭农场”模式、柒瑞结农场“合作种植”模式;总结了园区发展存在的主要问题,即生产基础薄弱、产品生产存在盲目性、专业技术人才缺乏、运营管理不足。基于此,可以通过加大政策扶持、加强产销衔接、注重人才培养、提升园区运营管理能力等方式解决现有园区发展问题。可以预期,随着设施农业园区基础设施建设、人才培养、管理制度等方面的完善与创新,未来农业园区将成为农业农村发展的重要载体,将支持现代农业快速、高质量发展。

The Impact of Bias Row Noise to Photometric Accuracy:Case Study Based on a Scientific CMOS Detector

We tested a new model of CMOS detector manufactured by the Gpixel Inc,for potential space astronomical application.In laboratory,we obtain some bias images under the typical application environment.In these bias images,clear random row noise pattern is observed.The row noise also contains some characteristic spatial frequencies.We quantitatively estimated the impact of this feature to photometric measurements,by making simulated images.We compared different bias noise types under strict parameter control.The result shows the row noise will significantly deteriorate the photometric accuracy.It effectively increases the readout noise by a factor of2-10.However,if it is properly removed,the image quality and photometric accuracy will be significantly improved.

Gut microbiota dysbiosis in Chinese children with type 1 diabetes mellitus:An observational study

BACKGROUND Gut microbiota dysbiosis is reportedly actively involved in autoimmune diseases such as type 1 diabetes mellitus(T1DM).However,the alterations in the gut microbiota and their correlation with fasting blood glucose(FBG)in Chinese children with T1DM remain unclear.AIM To investigate alterations in the gut microbiota in Chinese children with T1DM and their associations with clinical indicators.METHODS Samples from 51 children with T1DM and 47 age-matched and gender-matched healthy controls were obtained,to explore the structural and functional alterations in the fecal microbiota.The V3-V4 regions of the 16S rRNA gene were sequenced on a MiSeq instrument,and the association with FBG were analyzed.RESULTS We found that the bacterial diversity was significantly increased in the T1DMassociated fecal microbiota,and changes in the microbial composition were observed at different taxonomic levels.The T1DM-reduced differential taxa,such as Bacteroides vulgatus ATCC8482,Bacteroides ovatus,Bacteroides xylanisolvens,and Flavonifractor plautii,were negatively correlated with FBG,while the T1DMenriched taxa,such as Blautia,Eubacterium hallii group,Anaerostipes hadrus,and Dorea longicatena,were positively correlated with FBG.Bacteroides vulgatus ATCC8482,Bacteroides ovatus,the Eubacterium hallii group,and Anaerostipes hadrus,either alone or in combination,could be used as noninvasive diagnostic biomarkers to discriminate children with T1DM from healthy controls.In addition,the functional changes in the T1DM-associated fecal microbiota also suggest that these fecal microbes were associated with altered functions and metabolic activities,such as glycan biosynthesis and metabolism and lipid metabolism,which might play vital roles in the pathogenesis and development of T1DM.CONCLUSION Our present comprehensive investigation of the T1DM-associated fecal microbiota provides novel insights into the pathogenesis of the disease and sheds light on the diagnosis and treatment of T1DM.

Establishment of a pattern recognition metabolomics model for the diagnosis of hepatocellular carcinoma

BACKGROUND Early diagnosis of hepatocellular carcinoma may help to ensure that patients have a chance for long-term survival;however,currently available biomarkers lack sensitivity and specificity.AIM To characterize the serum metabolome of hepatocellular carcinoma in order to develop a new metabolomics diagnostic model and identifying novel biomarkers for screening hepatocellular carcinoma based on the pattern recognition method.METHODS Ultra-performance liquid chromatography-mass spectroscopy was used to characterize the serum metabolome of hepatocellular carcinoma(n=30)and cirrhosis(n=29)patients,followed by sequential feature selection combined with linear discriminant analysis to process the multivariate data.RESULTS The concentrations of most metabolites,including proline,were lower in patients with hepatocellular carcinoma,whereas the hydroxypurine levels were higher in these patients.As ordinary analysis models failed to discriminate hepatocellular carcinoma from cirrhosis,pattern recognition analysis was used to establish a pattern recognition model that included hydroxypurine and proline.The leaveone-out cross-validation accuracy and area under the receiver operating characteristic curve analysis were 95.00%and 0.90[95%Confidence Interval(CI):0.81-0.99]for the training set,respectively,and 78.95%and 0.84(95%CI:0.67-1.00)for the validation set,respectively.In contrast,forα-fetoprotein,the accuracy and area under the receiver operating characteristic curve were 65.00%and 0.69(95%CI:0.52-0.86)for the training set,respectively,and 68.42%and 0.68(95%CI:0.41-0.94)for the validation set,respectively.The Z test revealed that the area under the curve of the linear discriminant analysis model was significantly higher than the area under the curve ofα-fetoprotein(P<0.05)in both the training and validation sets.CONCLUSION Hydroxypurine and proline might be novel biomarkers for hepatocellular carcinoma,and this disease could be diagnosed by the metabolomics model based on pattern recognition.

Characteristics of Climate Change in the Lake Basin Area of Gangcha County

This paper mainly was based on the average temperature,precipitation,humidity,and wind direction of Gangcha county from 1960 to 2013.By using wavelet analysis and Mann-Kendall(M-K)mutation analysis,specifically analyzed the climate change characteristics in the lake basin area of Gangcha county.The result showed that the climatic change in the lake basin area of Gangcha county is noticeable.The average temperature,average minimum temperature,average maximum temperature,and evaporation showed an increasing trend.But the evaporation in the study area was higher than precipitation.The average relative humidity showed a decreasing trend.And the sunshine and the average wind speed percentage showed a significant decreasing trend.Utilizing the Morelet wavelet,The time series of annual mean temperature,annual evaporation and annual sunshine percentage all have quasi3a and quasi4A periods,and the annual mean precipitation has quasi2–3A,quasi2–5A and quasi2–6A periods,which appear in 1996–2005,1962–1978 and 1978–1996 respectively.The mean annual relative humidity has obvious quasi2–7A and quasi3A time series,which appear from 1960 to 1996,1997 to 2005 and after 2008,respectively.The annual mean wind speed has quasi3–4A and quasi5A time series characteristics,which appear in 1964–1967,1984–1995,after 2009 and 1971–1983,respectively.Through Mann-Kendall(M-K)mutation analysis,it is found that the mutations of evaporation and the average speed of wind are significant.The mutation of evaporation started in 2004,and the mutation of average started in 2003.

Correction to“Gut microbiota dysbiosis in Chinese children with type 1 diabetes mellitus:An observational study”

Liu X,Cheng YW,Shao L,Sun SH,Wu J,Song QH,Zou HS,Ling ZX.Gut microbiota dysbiosis in Chinese children with type 1 diabetes mellitus:An observational study.World J Gastroenterol 2021;27(19):2394-2414[PMID:34040330 DOI:10.3748/wjg.v27.i19.2394].In this article,the affiliation of the first author was wrong.Xia Liu,the first author,belonged to Department of Intensive Care Unit,The First Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou,310003,China.

温室果菜自动落蔓疏蔓系统设计与试验

为了提高设施果菜落蔓及疏蔓作业机械化、自动化程度,减轻劳动强度,提高作业效率,提出了融合落蔓及疏蔓功能的系统方案,并对关键部件进行设计且制作了样机。系统主要包括吊蔓轴、落蔓器、落蔓传动系统和疏蔓传动系统,通过吊蔓轴转动和水平移动实现落蔓和疏蔓。重点对吊蔓轴3种铰支约束状态下进行了静力学分析,结果表明:1/3处铰支约束的最大形变量不超过2mm,满足日光温室8m长种植系统落蔓需求。对落蔓器不同动摩擦因数进行仿真分析表明:随着动摩擦因数增大,最大工作角度逐渐减小,到达稳定工作状态时间变长,能够完成落蔓作业。温室生产试验结果表明:系统落蔓速度为3.47~6.91mm/s、疏蔓速度为3.35~6.81mm/s、疏蔓宽度为263mm时,可满足温室落蔓和疏蔓作业要求,实现了落蔓、疏蔓的自动化管理,提升了温室果菜生产自动化水平。

工业互联网在白酒行业的应用探讨

古井通过信息化建设,建立了一个工业互联网平台,完成了从供应商采购、酿酒生产、灌装到售后的全程质量管控,并且可以追溯环节的产品质量相关信息,完成过程监控和非正常告警控制功能,从而有效地完成白酒质量安全性的顺向追踪、逆向溯源和风险监管,确保质量安全性工作主体负责地落到实处。

成渝地区双城经济圈新能源汽车产业协同发展的思考与研究——以成都市为例

成渝双城经济圈的形成,使成渝区域经济协调发展成为一个重大课题。实现"成渝双城经济圈"的目标,必须以科学、合理的方式推进区域内的工业合作。虽然新能源汽车行业是促进两地经济发展的亮点,但仍然存在着行业结构同质化、区域间的恶性竞争、基础设施薄弱等问题。因此,本文从新的地域主义角度出发,就两地新能源产业的协调发展问题,提出了相应的政策建议。

Sulfate-assisted Ni/Fe-based electrodes for anion exchange membrane saline splitting

Saline water electrolysis is an appealing strategy for hydrogen production,attracting more attention in recent years.NiFe-based electrodes exhibit promise as catalysts for saline water electrolysis.Nevertheless,they suffer from the inferior service life of the oxygen evolution reaction(OER).Herein,we report an oxygen-evolution electrode consisting of a sulfate-modulated nickel-iron hydroxide(NiFeOOH)affording as the catalytic active layer and Fe-Ni_(3)S_(2) as the corrosion-proof layer.The developed electrode only requires overpotentials of 220 and 292 mV to deliver the current density of 10 and 500 mA·cm^(−2),respectively.More importantly,it presents long-term stability exceeding 140 and 100 h in 1 M KOH at high current densities of 500 and 1000 mA·cm^(−2),respectively,as well as 120 h for saline water electrolysis at 100 mA·cm^(−2).Experimental results reveal that the generated sulfate plays an indispensable role in improving stability and corrosion resistance.We assembled and tested an anion exchange membrane electrolyzer with Pt/C and NiFeS/NIF as the cathode and anode,respectively,under industrial conditions.This overall water-splitting electrolyzer achieves an impressive energy conversion efficiency of 75%±0.5%.This report offers fresh insights into the design of stable NiFe-based electrodes,which may further promote its practical applications for saline water electrolysis.

HiFAST:An HIdata calibration and imaging pipeline for FAST

The Five-hundred-meter Aperture Spherical radio Telescope(FAST)has the largest aperture and a 19-beam L-band receiver,making it powerful for investigating the neutral hydrogen atomic gas(HI)in the universe.We present HiFAST(https://hifast.readthedocs.io),a dedicated,modular,and self-contained calibration and imaging pipeline for processing the H?data of FAST.The pipeline consists of frequency-dependent noise diode calibration,baseline fitting,standing wave removal using an FFT-based method,flux density calibration,stray radiation correction,and gridding to produce data cubes.These modules can be combined as needed to process the data from most FAST observation modes:tracking,drift scanning,On-The-Fly mapping,and most of their variants.With HiFAST,the root-mean-square(RMS)noises of the calibrated spectra from all 19 beams were only slightly(~5%)higher than the theoretical expectation.The results for the extended source M33 and the point sources are consistent with the results from Arecibo.The moment maps(0,1 and 2)of M33 agree well with the results from the Arecibo Galaxy Environment Survey(AGES)with a fractional difference of less than 10%.For a common sample of 221 sources with signal-to-noise ratio S/N>10 from the Arecibo Legacy Fast ALFA(ALFALFA)survey,the mean value of fractional difference in the integrated flux density,Sint,between the two datasets is approximately 0.005%,with a dispersion of 15.4%.Further checks on the integrated flux density of 23 sources with seven observations indicate that the variance in the flux density of the source with luminous objects(Sint>2.5 Jy km s-1)is less than 5%.Our tests suggest that the FAST telescope,with the efficient,precise,and user-friendly pipeline HiFAST,will yield numerous significant scientific findings in the investigation of the H?in the universe.

网络靶标理论、关键技术与中医药应用

中医药强调辨证论治、方剂配伍,具有整体调节的特点。“网络靶标”理论突破了“单靶标”还原分析研究模式的局限,强调以疾病或证候生物网络为靶标的综合效应来表征中药方剂的整体调节机制,成为中医药网络药理学这一新兴学科的核心理论。基于网络靶标原创理论,本课题组历经20余年,发展出一套以高精度智能计算为核心,融合实验、临床等研究方法的中西医药分子网络导航系统——UNIQ系统,为从信息和系统的角度解读中医药科学原理、促进中医药精准诊疗与精准研发提供了有力支撑。目前,UNIQ系统已应用于中药上市前研发和上市后评价、病证生物标志物发现、智能诊疗设备研制,为网络药理学从“分析”迈向“证据”、从基础走向应用提供了有效范例,也为人工智能、大数据时代的中医药守正创新提供了新思路和新方法。

中国农业产能国际合作展望

产能国际合作是中国农业现代化"十三五"时期优化农业对外合作布局的主要领域之一,也被列入《共同推进"一带一路"建设农业合作的愿景与行动》。分析了农业产能国际合作内涵,总结了中国农业产能国际合作现状、产能国内供需形势以及国际合作形式。产能合作已经成为中国经济外交的新高地,也是对外开放升级的新支点。

脑动脉瘤介入栓塞术围术期患者行快速康复外科护理的应用价值

目的探讨脑动脉瘤介入栓塞术中采取快速康复外科护理在围术期的干预效果及其对并发症发生情况的影响。方法选取2018年1月~2019年12月我院60例脑动脉瘤介入栓塞术患者,根据护理方法的不同分为两组,对照组采取常规干预,观察组采取快速康复外科护理干预。观察两组住院时间、下床时间、住院费用、并发症发生率、复发颅内出血率及护理满意度。结果观察组住院时间、下床时间及住院费用均少于对照组;对照组的护理满意度低于观察组,差异均具有统计学意义(P<0.05);观察组复发颅内出血率与对照组的差异无统计学意义(P>0.05)。结论脑动脉瘤介入栓塞术中行快速康复外科护理可以缩短患者的下床时间及住院时间,减少患者并发症的发生,对患者的预后有着较好的影响,有利于患者的恢复。

永生化人肝细胞HepZJ的临床前急性毒性评价

背景:肝细胞移植、肝脏组织工程与生物人工肝的研究为肝衰竭患者带来福音,然而截至目前仍未发现十分合适的种子细胞。课题组前期自行构建人永生化肝细胞系HepZJ作为新的种子细胞,并进行了临床前安全性评价研究。目的:对新型永生化人肝细胞系HepZJ进行急性毒性研究,为HepZJ应用于临床可能发生的毒副反应以及设计安全剂量提供参考依据。方法:在HepZJ细胞急性毒性研究中,将低、中、高(5×10~6,5×10~7,8.25×10~7)3个剂量组的HepZJ细胞悬液和空白对照溶液经尾静脉单次注射进入SD大鼠体内,观察大鼠的临床症状和体质量变化,注射结束后第1天以及第14天剖检进行血液学相关检验、大体病理和免疫组化检查,综合分析该细胞系的毒副反应和安全剂量。结果与结论:在HepZJ细胞急性毒性研究中,高剂量组大鼠注射结束时出现1只死亡,与对照组比较,血常规、凝血功能和血清生化部分指标差异有显著性意义(P <0.05);14 d后各项检查差异无显著性意义(P> 0.05)。低剂量组和中剂量组大鼠与对照组在临床症状、体质量变化、血液指标和大体病理形态上差异均无显著性意义。结果表明,永生化人肝细胞系HepZJ单次注射安全剂量为5×10~7/只,无明显毒副反应,剂量过大时可出现明显毒副反应,包括炎症应激、凝血障碍和肝功能损伤等,甚至死亡。因此,只有准确控制HepZJ的使用剂量,才能保证该细胞的临床应用安全性。

基于异步通信的综合模块化车辆电子系统软件重构

随着综合模块化车辆电子系统的逐步应用,需要进一步研究其中的系统管理及软件重构技术,以提高车辆的故障处理能力及系统可靠性水平。研究联合标准化航空电子系统架构委员会(ASAAC)航空电子系统标准中的系统管理技术,以此为基础针对车辆电子系统的特点设计及实现一种基于异步通信的系统管理及软件重构方案,给出系统管理配置的实例及系统管理软件、模块管理软件的详细说明,并在桌面和嵌入式环境下完成了试验验证。研究和试验结果表明,该方案可以有效地降低多处理模块ASAAC系统管理所使用的通信负荷,较好地应对应用软件异常退出、模块断网或断电、软件重复运行等车辆电子系统中的常见故障,能在这些有故障的情况下保证系统应用软件运行的可靠性和完备性。

基于3D打印的仪表罩壳注塑模具随形冷却水路设计

以典型产品仪表罩壳为研究对象,借助Moldflow模流仿真软件,进行产品随形冷却水路的设计,对比分析传统冷却水路与三维(3D)打印随形冷却水路设计方案的冷却分析结果,以此评估随形冷却水路在实际应用中的优势,为随形冷却水路的设计提供理论性的参考指导,并为该产品在实际生产中提供优化的模具冷却设计方案。结果表明,与传统冷却水路相比,随形冷却水路方案的冷却效率最多可提高31. 6%,产品表面温度均匀性最多可提高44. 6%。

一种新的抗生素联合用药方案清除细胞支原体污染的研究

目的支原体污染是细胞培养过程中最为常见也较为棘手的问题,因此,解决细胞支原体污染问题存在必要性和迫切性。但是目前可用的商品化去除支原体试剂选择少,价格贵,本研究拟探索一种操作简单,成本低且去除支原体效果佳的抗生素联合用药方案。方法选取四种抗生素药物联合用药:红霉素、氯霉素、泰乐菌素和环丙沙星,连续12天处理培养支原体污染的C3A肝癌细胞。首先通过倒置显微镜观察加药处理后,C3A细胞内颗粒、形态等变化;加药处理C3A细胞后通过CCK-8法检测其活率。其次,进行支原体聚合酶链式反应(PCR)检测和荧光染色,评价抗生素联合用药方案去除支原体的有效性。最后,实时荧光定量PCR(qPCR)方法检测C3A细胞主要功能基因角蛋白(CK18)、白蛋白(ALB)、氨甲酰磷酸合成酶l(CPS1)和细胞色素P450酶系1A2(CYP1A2)等功能基因的表达恢复情况。结果经抗生素联合用药处理后,细胞形态更为规整,胞内颗粒显著减少,形态透亮折光度增强等特征变化,对C3A细胞活率无明显影响,抗生素联合用药组PCR电泳结果显示没有支原体DNA条带,同时荧光结果表明没有出现其余点状或者串点的支原体荧光,支原体去除后功能基因CK18、ALB、CPS1和CYP1A2相对表达量均升高。结论新的抗生素联合用药方案能够达到支原体去除效果,C3A细胞活率维持较好,去除支原体后细胞主要功能基因表达可得到恢复提升。