Although putrescine(PUT)has been linked to plants'responses to cadmium(Cd)stress,the exact mechanism is still elusive.In the current investigation,the endogenous PUT concentration in rice roots was enhanced by Cd ...Although putrescine(PUT)has been linked to plants'responses to cadmium(Cd)stress,the exact mechanism is still elusive.In the current investigation,the endogenous PUT concentration in rice roots was enhanced by Cd stress.Exogenous PUT increased root cell wall hemicellulose levels,which in turn increased its Cd binding capacity,concurrently decreasing the transcription level of genes such as OsNRAMP1 and OsCd1 that are responsible for root Cd absorption.As a result。展开更多
Background: The diagnosis of drug-induced autoimmune hepatitis(DIAIH) and its differentiation from idiopathic autoimmune hepatitis(AIH) is challenging. This study aimed to differentiate DIAIH from AIH by comparing the...Background: The diagnosis of drug-induced autoimmune hepatitis(DIAIH) and its differentiation from idiopathic autoimmune hepatitis(AIH) is challenging. This study aimed to differentiate DIAIH from AIH by comparing the biochemical changes, histological features, and frequencies of CD4~+Foxp3~+CD25+/-regulatory T cells(Tregs) in liver tissues or peripheral blood lymphocytes.Methods: A total of 15 DIAIH patients and 24 AIH patients who underwent liver biopsies at initial presentation were enrolled in this study. The liver histological changes were assessed by HE staining. The phenotypic recognition and distribution of CD4~+Foxp3~+CD25+/-Tregs in liver tissues were evaluated by single/double immunostains in serial sections. The CD4~+Foxp3~+CD25+/-Tregs in peripheral blood were analyzed by flow cytometry.Results: The median values of ALT and AST were 404.50 U/L and 454.10 U/L in DIAIH patients and309.50 U/L and 315.00 U/L in AIH patients, respectively. More importantly, for the first time we found that patients with DIAIH had higher levels of serum ALT and AST, more severe degree of lobular inflammation,higher frequencies of zone 3 necrosis and higher number of lobular CD4~+Foxp3~+CD25~-Tregs compared with AIH(P < 0.05). Furthermore, there were positive correlations in DIAIH between the degree of lobular inflammation and either the AST/ALT level or the number of lobular CD4~+Foxp3~+CD25~-Tregs(P < 0.05).However, the frequency of peripheral blood CD4~+Foxp3~+CD25+/-Tregs were not significantly different between DIAIH and AIH.Conclusions: The differences of ALT, AST and the number of lobular CD4~+Foxp3~+CD25~-Tregs between patients with DIAIH and those with AIH are clinically helpful in differentiating these two diseases in their early stage.展开更多
主从区块链是一种面向领域的、采用高效密码学原理进行大数据可信化通信及存储的新型信息处理技术.随着领域数据规模的指数级增长,现有主从区块链系统存在的查询效率低、溯源时间长等问题愈发严重.针对这些问题,提出一种面向主从区块链...主从区块链是一种面向领域的、采用高效密码学原理进行大数据可信化通信及存储的新型信息处理技术.随着领域数据规模的指数级增长,现有主从区块链系统存在的查询效率低、溯源时间长等问题愈发严重.针对这些问题,提出一种面向主从区块链的多级索引构建方法(multi-level index construction method for master-slave blockchain,MSMLI).首先,MSMLI引入权重矩阵,基于主链结构将整个主从区块链进行分片,并对各个分片进行权重赋值;其次,针对每个分片内的主区块链,提出基于跳跃一致性哈希的主链索引构建方法(master chain index construction method based on jump consistent Hash,JHMI),输入节点关键值和索引槽位数量,输出主链索引;最后,引入布隆过滤器,改进基于列的选择函数,对各个主区块对应的从属区块链构建2级复合索引.在3种约束条件和2类数据集上的实验结果表明,MSMLI对比现有方法,平均能够缩减9.28%的索引构建时间,提升12.07%的查询效率,同时降低24.4%的内存开销.展开更多
Mobile Edge Computing(MEC)is a technology for the fifth-generation(5G)wireless communications to enable User Equipment(UE)to offload tasks to servers deployed at the edge of network.However,taking both delay and energ...Mobile Edge Computing(MEC)is a technology for the fifth-generation(5G)wireless communications to enable User Equipment(UE)to offload tasks to servers deployed at the edge of network.However,taking both delay and energy consumption into consideration in the 5G MEC system is usually complex and contradictory.Non-orthogonal multiple access(NOMA)enable more UEs to offload their computing tasks to MEC servers using the same spectrum resources to enhance the spectrum efficiency for 5G,which makes the problem even more complex in the NOMA-MEC system.In this work,a system utility maximization model is present to NOMA-MEC system,and two optimization algorithms based on Newton method and greedy algorithm respectively are proposed to jointly optimize the computing resource allocation,SIC order,transmission time slot allocation,which can easily achieve a better trade-off between the delay and energy consumption.The simulation results prove that the proposed method is effective for NOMA-MEC systems.展开更多
BACKGROUND Low-grade myofibroblastic sarcoma(LGMS)is a rare spindle cell sarcoma espe-cially in the pancreas,with myofibroblastic differentiation.Hitherto,only a few cases have been reported.CASE SUMMARY Herein,we rep...BACKGROUND Low-grade myofibroblastic sarcoma(LGMS)is a rare spindle cell sarcoma espe-cially in the pancreas,with myofibroblastic differentiation.Hitherto,only a few cases have been reported.CASE SUMMARY Herein,we report a case involving the discovery of a pancreatic mass detected during a routine physical examination.Subsequent imaging and pathological tests of the patient led to the diagnosis of LGMS of the pancreas.Following surgical intervention,the patient experienced recurrence and metastasis.Conventional treatment is not effective for postoperative recurrent pancreatic LGMS with multiple metastases.After communicating with the patients and their families,informed consent was obtained for the treatment of anlotinib combined with pembrolizumab.Evaluation of imaging and clinical symptoms post-treatment revealed a relatively favorable response to the combination of anlotinib and pembrolizumab.CONCLUSION Based on the comprehensive literature review,our report aimed to provide evidence for a better understanding of the disease characteristics,diagnostic criteria,imaging findings,and identification of LGMS.And explore novel treatment strategies for this disease.展开更多
Ras-associated protein-1(Rap1), a small GTPase in the Ras-related protein family, is an important regulator of basic cellular functions(e.g., formation and control of cell adhesions and junctions), cellular migration,...Ras-associated protein-1(Rap1), a small GTPase in the Ras-related protein family, is an important regulator of basic cellular functions(e.g., formation and control of cell adhesions and junctions), cellular migration, and polarization. Through its interaction with other proteins, Rap1 plays many roles during cell invasion and metastasis in different cancers. The basic function of Rap1 is straightforward; it acts as a switch during cellular signaling transduction and regulated by its binding to either guanosine triphosphate(GTP) or guanosine diphosphate(GDP). However, its remarkably diverse function is rendered by its interplay with a large number of distinct Rap guanine nucleotide exchange factors and Rap GTPase activating proteins. This review summarizes the mechanisms by which Rap1 signaling can regulate cell invasion and metastasis, focusing on its roles in integrin and cadherin regulation, Rho GTPase control, and matrix metalloproteinase expression.展开更多
AIM： To investigate the regulatory mechanism of glycogen synthase kinase 3β（GSK3β） in epithelialmesenchymal transition（EMT） process after proliferative vitreoretinopathy（PVR） induction. METHODS： Experimenta...AIM： To investigate the regulatory mechanism of glycogen synthase kinase 3β（GSK3β） in epithelialmesenchymal transition（EMT） process after proliferative vitreoretinopathy（PVR） induction. METHODS： Experimental PVR was induced by intravitreal injection of retinal pigment epithelium（RPE） cells in the eyes of rabbits. A PI3 K/Akt inhibitor（wortmannin） and a GSK3β inhibitor（Li Cl） were also injected at different time during PVR progress. Electroretinogram（ERG）, ocular fundus photographs, and B-scan ultrasonography were used to observe the PVR progress. Western blot test on the extracted retina were performed at 1, 2, 4 wk. The expression of the mesenchymal marker vimentin was determined by immunohistochemistry. Toxicity of wortmannin and Li Cl were evaluated by ERG and Td Tmediated d UTP nick-end labeling（TUNEL） assay. The vitreous was also collected for metabolomic analysis. RESULTS： Experimental PVR could significantly lead to EMT, along with the suppressed expression of GSK3β and the activation of Wnt/β-catenin and PI3 K/Akt pathways. It was verified that upregulating the expression of GSK3β could effectively inhibit EMT process by suppressing Wnt/β-catenin and PI3 K/Akt pathways. CONCLUSION： GSK3β effectively inhibits EMT via the Wnt/β-catenin and PI3 K/Akt pathways. GSK3β may be regarded as a promising target of experimental PVR inhibition.展开更多
基金supported by the Hainan Yazhou Bay Seed Laboratory Project,China(Grant No.B21HJ0220)the National Natural Science Foundation of China(Grant No.42020104004)+3 种基金the Field Frontier Program of the Institute of Soil Science,China(Grant No.ISSASIP2215)the Agricultural Science and Technology Independent Innovation Fund Project of Jiangsu Province,China(Grant No.CX(21)2034)the Key Research and Development Project of Jiangsu Province,China(Grant No.BE2021717)the Special Fund Project of Soil Pollution Prevention and Control(Xinyi Agriculture and Rural Bureau,New Collection List(2021)1468)。
文摘Although putrescine(PUT)has been linked to plants'responses to cadmium(Cd)stress,the exact mechanism is still elusive.In the current investigation,the endogenous PUT concentration in rice roots was enhanced by Cd stress.Exogenous PUT increased root cell wall hemicellulose levels,which in turn increased its Cd binding capacity,concurrently decreasing the transcription level of genes such as OsNRAMP1 and OsCd1 that are responsible for root Cd absorption.As a result。
基金supported by a grant from the National Natural Science Foundation of China(81270544)
文摘Background: The diagnosis of drug-induced autoimmune hepatitis(DIAIH) and its differentiation from idiopathic autoimmune hepatitis(AIH) is challenging. This study aimed to differentiate DIAIH from AIH by comparing the biochemical changes, histological features, and frequencies of CD4~+Foxp3~+CD25+/-regulatory T cells(Tregs) in liver tissues or peripheral blood lymphocytes.Methods: A total of 15 DIAIH patients and 24 AIH patients who underwent liver biopsies at initial presentation were enrolled in this study. The liver histological changes were assessed by HE staining. The phenotypic recognition and distribution of CD4~+Foxp3~+CD25+/-Tregs in liver tissues were evaluated by single/double immunostains in serial sections. The CD4~+Foxp3~+CD25+/-Tregs in peripheral blood were analyzed by flow cytometry.Results: The median values of ALT and AST were 404.50 U/L and 454.10 U/L in DIAIH patients and309.50 U/L and 315.00 U/L in AIH patients, respectively. More importantly, for the first time we found that patients with DIAIH had higher levels of serum ALT and AST, more severe degree of lobular inflammation,higher frequencies of zone 3 necrosis and higher number of lobular CD4~+Foxp3~+CD25~-Tregs compared with AIH(P < 0.05). Furthermore, there were positive correlations in DIAIH between the degree of lobular inflammation and either the AST/ALT level or the number of lobular CD4~+Foxp3~+CD25~-Tregs(P < 0.05).However, the frequency of peripheral blood CD4~+Foxp3~+CD25+/-Tregs were not significantly different between DIAIH and AIH.Conclusions: The differences of ALT, AST and the number of lobular CD4~+Foxp3~+CD25~-Tregs between patients with DIAIH and those with AIH are clinically helpful in differentiating these two diseases in their early stage.
文摘主从区块链是一种面向领域的、采用高效密码学原理进行大数据可信化通信及存储的新型信息处理技术.随着领域数据规模的指数级增长,现有主从区块链系统存在的查询效率低、溯源时间长等问题愈发严重.针对这些问题,提出一种面向主从区块链的多级索引构建方法(multi-level index construction method for master-slave blockchain,MSMLI).首先,MSMLI引入权重矩阵,基于主链结构将整个主从区块链进行分片,并对各个分片进行权重赋值;其次,针对每个分片内的主区块链,提出基于跳跃一致性哈希的主链索引构建方法(master chain index construction method based on jump consistent Hash,JHMI),输入节点关键值和索引槽位数量,输出主链索引;最后,引入布隆过滤器,改进基于列的选择函数,对各个主区块对应的从属区块链构建2级复合索引.在3种约束条件和2类数据集上的实验结果表明,MSMLI对比现有方法,平均能够缩减9.28%的索引构建时间,提升12.07%的查询效率,同时降低24.4%的内存开销.
文摘Mobile Edge Computing(MEC)is a technology for the fifth-generation(5G)wireless communications to enable User Equipment(UE)to offload tasks to servers deployed at the edge of network.However,taking both delay and energy consumption into consideration in the 5G MEC system is usually complex and contradictory.Non-orthogonal multiple access(NOMA)enable more UEs to offload their computing tasks to MEC servers using the same spectrum resources to enhance the spectrum efficiency for 5G,which makes the problem even more complex in the NOMA-MEC system.In this work,a system utility maximization model is present to NOMA-MEC system,and two optimization algorithms based on Newton method and greedy algorithm respectively are proposed to jointly optimize the computing resource allocation,SIC order,transmission time slot allocation,which can easily achieve a better trade-off between the delay and energy consumption.The simulation results prove that the proposed method is effective for NOMA-MEC systems.
文摘BACKGROUND Low-grade myofibroblastic sarcoma(LGMS)is a rare spindle cell sarcoma espe-cially in the pancreas,with myofibroblastic differentiation.Hitherto,only a few cases have been reported.CASE SUMMARY Herein,we report a case involving the discovery of a pancreatic mass detected during a routine physical examination.Subsequent imaging and pathological tests of the patient led to the diagnosis of LGMS of the pancreas.Following surgical intervention,the patient experienced recurrence and metastasis.Conventional treatment is not effective for postoperative recurrent pancreatic LGMS with multiple metastases.After communicating with the patients and their families,informed consent was obtained for the treatment of anlotinib combined with pembrolizumab.Evaluation of imaging and clinical symptoms post-treatment revealed a relatively favorable response to the combination of anlotinib and pembrolizumab.CONCLUSION Based on the comprehensive literature review,our report aimed to provide evidence for a better understanding of the disease characteristics,diagnostic criteria,imaging findings,and identification of LGMS.And explore novel treatment strategies for this disease.
基金supported by grants from the National Natural Science Foundation of China(Grant No.31271504 and 31471310)the Shenzhen Science and Technology Innovation Committee,China(Grant No.JCYJ2013040 1144744187)
文摘Ras-associated protein-1(Rap1), a small GTPase in the Ras-related protein family, is an important regulator of basic cellular functions(e.g., formation and control of cell adhesions and junctions), cellular migration, and polarization. Through its interaction with other proteins, Rap1 plays many roles during cell invasion and metastasis in different cancers. The basic function of Rap1 is straightforward; it acts as a switch during cellular signaling transduction and regulated by its binding to either guanosine triphosphate(GTP) or guanosine diphosphate(GDP). However, its remarkably diverse function is rendered by its interplay with a large number of distinct Rap guanine nucleotide exchange factors and Rap GTPase activating proteins. This review summarizes the mechanisms by which Rap1 signaling can regulate cell invasion and metastasis, focusing on its roles in integrin and cadherin regulation, Rho GTPase control, and matrix metalloproteinase expression.
基金Supported by the National Natural Science Foundation of China(No.81371039)Shanghai Natural Science Foundation(No.18ZR1440200)
文摘AIM： To investigate the regulatory mechanism of glycogen synthase kinase 3β（GSK3β） in epithelialmesenchymal transition（EMT） process after proliferative vitreoretinopathy（PVR） induction. METHODS： Experimental PVR was induced by intravitreal injection of retinal pigment epithelium（RPE） cells in the eyes of rabbits. A PI3 K/Akt inhibitor（wortmannin） and a GSK3β inhibitor（Li Cl） were also injected at different time during PVR progress. Electroretinogram（ERG）, ocular fundus photographs, and B-scan ultrasonography were used to observe the PVR progress. Western blot test on the extracted retina were performed at 1, 2, 4 wk. The expression of the mesenchymal marker vimentin was determined by immunohistochemistry. Toxicity of wortmannin and Li Cl were evaluated by ERG and Td Tmediated d UTP nick-end labeling（TUNEL） assay. The vitreous was also collected for metabolomic analysis. RESULTS： Experimental PVR could significantly lead to EMT, along with the suppressed expression of GSK3β and the activation of Wnt/β-catenin and PI3 K/Akt pathways. It was verified that upregulating the expression of GSK3β could effectively inhibit EMT process by suppressing Wnt/β-catenin and PI3 K/Akt pathways. CONCLUSION： GSK3β effectively inhibits EMT via the Wnt/β-catenin and PI3 K/Akt pathways. GSK3β may be regarded as a promising target of experimental PVR inhibition.
