目的通过动物模型来探讨外源性注射右美托咪定在大鼠脑缺血再灌注损伤过程中的保护作用,以及对铁死亡通路蛋白的影响。方法选择8周龄健康SPF级雄性SD大鼠45只,体质量约220 g。随机分为假手术组、模型组和右美托咪定组。改良Longa线栓法...目的通过动物模型来探讨外源性注射右美托咪定在大鼠脑缺血再灌注损伤过程中的保护作用,以及对铁死亡通路蛋白的影响。方法选择8周龄健康SPF级雄性SD大鼠45只,体质量约220 g。随机分为假手术组、模型组和右美托咪定组。改良Longa线栓法构建模型。24 h后取血肿周围组织原位末端标记法(TUNEL)染色检测神经元形态和凋亡率,氯代三苯基四氮唑(TTC)染色测定脑梗死体积,普鲁士蓝染色检测铁沉积量,酶联免疫吸附分析(ELISA)法检测丙二醛(MDA)、谷胱甘肽(GSH)和谷胱甘肽还原酶4(GPX4),Western blot法检测环氧合酶-2(COX-2)、血红素转运蛋白(HPC1)、转铁蛋白受体(TfR)和铁输出蛋白(FPN1)。结果与假手术组相比,模型组神经元凋亡率[(18.6±1.6)%vs(2.3±0.4)%]、脑梗死体积[(42.7±5.7)%vs(3.1±0.5)%]、铁沉积量[(35.1±4.4)%vs(2.6±0.3)%]、血清MDA水平[(42.52±6.63)mmol/L vs(15.63±3.26)mmol/L]、组织COX-2(0.79±0.13 vs 0.41±0.06)、HPC1(0.62±0.08 vs 0.34±0.03)和TfR蛋白(0.53±0.07 vs 0.24±0.03)相对表达量均明显升高,而血清GSH[(18.65±3.52)μmol/L vs(30.21±5.62)μmol/L]和GPX4水平[(24.52±4.65)μmol/L vs(39.63±6.03)μmol/L]及组织FPN1蛋白(0.36±0.03 vs 0.54±0.05)相对表达量明显降低(P<0.05)。与模型组相比,右美托咪定组神经元凋亡率[(9.1±1.1)%vs(18.6±2.3)%]、脑梗死体积[(22.3±3.2)%vs(42.7±6.1)%]、铁沉积量[(12.6±2.1)%vs(35.1±4.3)%]、血清MDA水平[(28.65±4.21)mmol/L vs(42.52±6.63)mmol/L]、组织COX-2(0.60±0.08 vs 0.79±0.13)、HPC1(0.55±0.06 vs 0.62±0.08)和TfR蛋白(0.41±0.05 vs 0.53±0.07)相对表达量明显降低,而血清GSH[(25.52±3.69)μmol/L vs(18.65±3.52)μmol/L]和GPX4水平[(33.26±5.25)μmol/L vs(24.52±4.65)μmol/L]及组织FPN1蛋白(0.42±0.04 vs 0.36±0.03)表达量明显升高(P<0.05)。结论右美托咪定可能通过影响铁死亡通路蛋白表达,进而参与大鼠脑缺血再灌注损伤的保护作用。展开更多
The secondary bud burst can cause around 10%-20%yield losses in black currants,an economically important crop in parts of Europe,Asia and North America.The metabolism of reactive oxygen species(ROS)has been linked to ...The secondary bud burst can cause around 10%-20%yield losses in black currants,an economically important crop in parts of Europe,Asia and North America.The metabolism of reactive oxygen species(ROS)has been linked to bud dormancy and its early release(secondary bud burst)in several fruit crops.But the relationship between ROS metabolism and the secondary bud burst is still not well understood in black currants.In the present study,two black currant cultivars(Adelinia and Heifeng)with opposing tendency of exhibiting the secondary bud burst were sprayed with abscisic acid(ABA)and gibberellic acid(GA_(3))to either inhibit or induce the secondary bud burst.The results showed that ABA inhibited the secondary bud burst by reducing the contents of ROS(H_(2)O_(2),O_(2)-·)in buds;decreasing the activities of superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT);and increasing the contents of oxidized glutathione(GSSG)and ascorbic acid(AsA).GA_(3) effectively induced the secondary bud burst by increasing ROS contents;increasing the activities of several antioxidant enzymes,such as SOD,POD,CAT,glutathione reductase(GR),ascorbate peroxidase(APX)and the contents of reduced glutathione(GSH);and decreasing the contents of AsA.The experimental results showed that GA_(3) treatment increased the content of ROS,accelerated the metabolism of reactive oxygen species,and promoted the second burst of black currants.However,ROS metabolism was at a low level under ABA treatment,and the buds remained dormant.These results suggested that ROS metabolism might play an important role in the two black currants of the secondary bud burst.展开更多
The excitation functions were measured in the reaction of 27Al+27Al at incident energies from 114MeV to 127MeV in steps of 200keV. The detection angles were continuously covered from 10o to 57° in the laboratory ...The excitation functions were measured in the reaction of 27Al+27Al at incident energies from 114MeV to 127MeV in steps of 200keV. The detection angles were continuously covered from 10o to 57° in the laboratory system. The energy autocorrelation functions of the dissipative fragments were analyzed by using different approaches. The nonself-averaging oscillations in the excitation functions were considered due to the angular momentum coherence and damping of the coherent nuclear rotation. The damping results from a quantum chaotic motion.展开更多
Measurements of the total reaction cross section for^(12-16)C,^(14-17)N and^(16-18)O on carbon target at intermediate energies were performed on RIBLL of HIRFL.A larger enhancement ofσR for^(15)C was observed than th...Measurements of the total reaction cross section for^(12-16)C,^(14-17)N and^(16-18)O on carbon target at intermediate energies were performed on RIBLL of HIRFL.A larger enhancement ofσR for^(15)C was observed than that for its neighbors.The analysis of these data indicates possible existence of an anomalous nuclear structure in^(15)C.A new quantity was suggested as the criterion to distinguish anomalous structure nuclei from normal nuclei.展开更多
Fragments produced in the reaction of 60 MeV/n^(18)O on Be have been measured experimentally.The isotopic distribution of fragmentation reaction is well reproduced by a modified statistical abrasion-ablation model It ...Fragments produced in the reaction of 60 MeV/n^(18)O on Be have been measured experimentally.The isotopic distribution of fragmentation reaction is well reproduced by a modified statistical abrasion-ablation model It is predicted that the isotopic distribution shifts toward the neutron rich side for neutron rich projectile,but this isospin effect decreases with the increase of the atomic number difference Z-Z_(A)and disappears at last,where Z and Z_(A)are atomic numbers of the isotopic distribution and the projectile respectively.展开更多
The experimental measurement of the total interaction cross sections of N,C,B,and Be isotopes on 12C target at medium energies is described.A new formula that gives a good description of the energy-range relationship ...The experimental measurement of the total interaction cross sections of N,C,B,and Be isotopes on 12C target at medium energies is described.A new formula that gives a good description of the energy-range relationship of energetic ions over a wide energy range is presented.The energy calibration of detectors is made by a Monte Carlo simulation method based on this new formula.展开更多
The excitation functions of dissipative products have been measured in the reaction 27Al+27Al at incident energies from 114 to 127MeV in steps of 200keV. Detection angles vary continuously from 10.4° to 57.4°...The excitation functions of dissipative products have been measured in the reaction 27Al+27Al at incident energies from 114 to 127MeV in steps of 200keV. Detection angles vary continuously from 10.4° to 57.4° in laboratory system. An angular coherent width more than 40° in center of mass is obtained. The results from the analysis of excitation functions with nonself-averaging are characterized by a quasi-periodic long-range energy structure embedded in the disorder Ericson fluctuation.展开更多
文摘目的通过动物模型来探讨外源性注射右美托咪定在大鼠脑缺血再灌注损伤过程中的保护作用,以及对铁死亡通路蛋白的影响。方法选择8周龄健康SPF级雄性SD大鼠45只,体质量约220 g。随机分为假手术组、模型组和右美托咪定组。改良Longa线栓法构建模型。24 h后取血肿周围组织原位末端标记法(TUNEL)染色检测神经元形态和凋亡率,氯代三苯基四氮唑(TTC)染色测定脑梗死体积,普鲁士蓝染色检测铁沉积量,酶联免疫吸附分析(ELISA)法检测丙二醛(MDA)、谷胱甘肽(GSH)和谷胱甘肽还原酶4(GPX4),Western blot法检测环氧合酶-2(COX-2)、血红素转运蛋白(HPC1)、转铁蛋白受体(TfR)和铁输出蛋白(FPN1)。结果与假手术组相比,模型组神经元凋亡率[(18.6±1.6)%vs(2.3±0.4)%]、脑梗死体积[(42.7±5.7)%vs(3.1±0.5)%]、铁沉积量[(35.1±4.4)%vs(2.6±0.3)%]、血清MDA水平[(42.52±6.63)mmol/L vs(15.63±3.26)mmol/L]、组织COX-2(0.79±0.13 vs 0.41±0.06)、HPC1(0.62±0.08 vs 0.34±0.03)和TfR蛋白(0.53±0.07 vs 0.24±0.03)相对表达量均明显升高,而血清GSH[(18.65±3.52)μmol/L vs(30.21±5.62)μmol/L]和GPX4水平[(24.52±4.65)μmol/L vs(39.63±6.03)μmol/L]及组织FPN1蛋白(0.36±0.03 vs 0.54±0.05)相对表达量明显降低(P<0.05)。与模型组相比,右美托咪定组神经元凋亡率[(9.1±1.1)%vs(18.6±2.3)%]、脑梗死体积[(22.3±3.2)%vs(42.7±6.1)%]、铁沉积量[(12.6±2.1)%vs(35.1±4.3)%]、血清MDA水平[(28.65±4.21)mmol/L vs(42.52±6.63)mmol/L]、组织COX-2(0.60±0.08 vs 0.79±0.13)、HPC1(0.55±0.06 vs 0.62±0.08)和TfR蛋白(0.41±0.05 vs 0.53±0.07)相对表达量明显降低,而血清GSH[(25.52±3.69)μmol/L vs(18.65±3.52)μmol/L]和GPX4水平[(33.26±5.25)μmol/L vs(24.52±4.65)μmol/L]及组织FPN1蛋白(0.42±0.04 vs 0.36±0.03)表达量明显升高(P<0.05)。结论右美托咪定可能通过影响铁死亡通路蛋白表达,进而参与大鼠脑缺血再灌注损伤的保护作用。
基金Supported by Academic Backbone Project of Northeast Agricultural University(20XG04)the National Key R&D Program of China(2018YFD1000200)Heilongjiang Province Postdoctoral Startup Fund(LBH-Q17029)。
文摘The secondary bud burst can cause around 10%-20%yield losses in black currants,an economically important crop in parts of Europe,Asia and North America.The metabolism of reactive oxygen species(ROS)has been linked to bud dormancy and its early release(secondary bud burst)in several fruit crops.But the relationship between ROS metabolism and the secondary bud burst is still not well understood in black currants.In the present study,two black currant cultivars(Adelinia and Heifeng)with opposing tendency of exhibiting the secondary bud burst were sprayed with abscisic acid(ABA)and gibberellic acid(GA_(3))to either inhibit or induce the secondary bud burst.The results showed that ABA inhibited the secondary bud burst by reducing the contents of ROS(H_(2)O_(2),O_(2)-·)in buds;decreasing the activities of superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT);and increasing the contents of oxidized glutathione(GSSG)and ascorbic acid(AsA).GA_(3) effectively induced the secondary bud burst by increasing ROS contents;increasing the activities of several antioxidant enzymes,such as SOD,POD,CAT,glutathione reductase(GR),ascorbate peroxidase(APX)and the contents of reduced glutathione(GSH);and decreasing the contents of AsA.The experimental results showed that GA_(3) treatment increased the content of ROS,accelerated the metabolism of reactive oxygen species,and promoted the second burst of black currants.However,ROS metabolism was at a low level under ABA treatment,and the buds remained dormant.These results suggested that ROS metabolism might play an important role in the two black currants of the secondary bud burst.
基金the National Natural Science Foundation (No.19775057), and by the Chinese Academyof Sciences and the Laboratori Nazionale del
文摘The excitation functions were measured in the reaction of 27Al+27Al at incident energies from 114MeV to 127MeV in steps of 200keV. The detection angles were continuously covered from 10o to 57° in the laboratory system. The energy autocorrelation functions of the dissipative fragments were analyzed by using different approaches. The nonself-averaging oscillations in the excitation functions were considered due to the angular momentum coherence and damping of the coherent nuclear rotation. The damping results from a quantum chaotic motion.
基金Supported by the National Natural Science Foundation of China for Distinguished Young Scholar under Grand No.19625513National Natural Science Foundation of China under Grant No.19675059+1 种基金Shanghai Science,Technology Development Fund under Grant No.96XD14011Major State Basic Research Development Program(G200077400).
文摘Measurements of the total reaction cross section for^(12-16)C,^(14-17)N and^(16-18)O on carbon target at intermediate energies were performed on RIBLL of HIRFL.A larger enhancement ofσR for^(15)C was observed than that for its neighbors.The analysis of these data indicates possible existence of an anomalous nuclear structure in^(15)C.A new quantity was suggested as the criterion to distinguish anomalous structure nuclei from normal nuclei.
基金Supported by the National Natural Science Foundation of China for Distinguished Young Scholar under Grand No.19625513National Natural Science Foundation of China under Grant No.19675059Shanghai Science and Technology Development Fund under Grant No.96XD14011.
文摘Fragments produced in the reaction of 60 MeV/n^(18)O on Be have been measured experimentally.The isotopic distribution of fragmentation reaction is well reproduced by a modified statistical abrasion-ablation model It is predicted that the isotopic distribution shifts toward the neutron rich side for neutron rich projectile,but this isospin effect decreases with the increase of the atomic number difference Z-Z_(A)and disappears at last,where Z and Z_(A)are atomic numbers of the isotopic distribution and the projectile respectively.
基金Supported by the National Science Foundation of China for Distinguished Young Scholar under Grand No.19625513.
文摘The experimental measurement of the total interaction cross sections of N,C,B,and Be isotopes on 12C target at medium energies is described.A new formula that gives a good description of the energy-range relationship of energetic ions over a wide energy range is presented.The energy calibration of detectors is made by a Monte Carlo simulation method based on this new formula.
基金the National Natural Science Foundation of China under Grant No.19775057the Chinese Academy of Sciences(No.KJ95T-03).
文摘The excitation functions of dissipative products have been measured in the reaction 27Al+27Al at incident energies from 114 to 127MeV in steps of 200keV. Detection angles vary continuously from 10.4° to 57.4° in laboratory system. An angular coherent width more than 40° in center of mass is obtained. The results from the analysis of excitation functions with nonself-averaging are characterized by a quasi-periodic long-range energy structure embedded in the disorder Ericson fluctuation.
