Multifunctional scaffolds with host defense peptides designed for regenerative endodontics are desirable nanobiotechnological tools for dentistry.Here,different scaffolds were tested for use during the pulp revascular...Multifunctional scaffolds with host defense peptides designed for regenerative endodontics are desirable nanobiotechnological tools for dentistry.Here,different scaffolds were tested for use during the pulp revascularization process,including poly(vinyl alcohol)-PVA hydrogels or resins,collagen hydrogels and poly(vinyl alcohol)PVA/Chitosan(PVA/CS)nanofibers.Based on time to degradation(21 days),nanofibers were chosen to be incorporated with ciprofloxacin and IDR-1002(each at 50 mg/g).Nanofibers containing ciprofloxacin and IDR-1002 had anti-biofilm activity against Enterococcus faecalis,Staphylococcus aureus and a multispecies oral biofilm,besides anti-inflammatory activities.The in vivo subcutaneous tissue response to tooth fragments filled with nanofibers demonstrated a pulp-like tissue formation,when compared to empty teeth fragments.Thus,we designed a strong antimicrobial,immunomodulatory and regenerative candidate for pulp revascularization and regeneration procedures.展开更多
基金supported by Conselho Nacional de Desenvolvimento Científico e Tecnologico(CNPq)(409196/2018-5),Coordenaçao de Aperfeiçoamento de Pessoal de Nível Superior(CAPES)(88887.202222/2018-00)Fundaçao de Apoioa Pesquisa do Distrito Federal(FAPDF)(00193-00000782/2021-63),and Fundaçao de Apoio ao Desenvolvimento do Ensino,Ciˆencia e Tecnologia do Estado de Mato Grosso do Sul(FUNDECT)(59/300.397/2015+2 种基金022/2018028973)In addition,this study was supported by a Canadian Institutes of Health Research Foundation grant FDN-154287 to R.E.W.Hancock.HE is the recipient of a UBC Killam Fellowship and a Research Trainee Award from the Michael Smith Foundation for Health Research(MSFHR).R.E.W.Hancock is a Canada Research Chair in Health and Genomics and a UBC Killam Professor.We also acknowledge Professor Marcelo Oliveira Rodrigues and Chemistry Institute(University of Brasilia)for all support.
文摘Multifunctional scaffolds with host defense peptides designed for regenerative endodontics are desirable nanobiotechnological tools for dentistry.Here,different scaffolds were tested for use during the pulp revascularization process,including poly(vinyl alcohol)-PVA hydrogels or resins,collagen hydrogels and poly(vinyl alcohol)PVA/Chitosan(PVA/CS)nanofibers.Based on time to degradation(21 days),nanofibers were chosen to be incorporated with ciprofloxacin and IDR-1002(each at 50 mg/g).Nanofibers containing ciprofloxacin and IDR-1002 had anti-biofilm activity against Enterococcus faecalis,Staphylococcus aureus and a multispecies oral biofilm,besides anti-inflammatory activities.The in vivo subcutaneous tissue response to tooth fragments filled with nanofibers demonstrated a pulp-like tissue formation,when compared to empty teeth fragments.Thus,we designed a strong antimicrobial,immunomodulatory and regenerative candidate for pulp revascularization and regeneration procedures.
