Porcine parvovirus(PPV) is one of the major causes of reproductive failure in pigs, which poses a great threat to the pig breeding industry and results in tremendous economic losses worldwide. Autophagy is the biologi...Porcine parvovirus(PPV) is one of the major causes of reproductive failure in pigs, which poses a great threat to the pig breeding industry and results in tremendous economic losses worldwide. Autophagy is the biological process of cell self-defense and self-protection. Despite many viruses can cause cell autophagy, when they enter cell or copied, the relationship between autophagy and PPV infection has not been reported. In this study, impact of autophagy after swine testicular(ST) cells infected by PPV was studied. Autophagy was demonstrated by the effective replication of PPV through transmission electron microscopy, immunofluorescence and western blot analysis. Moreover, autophagy was confirmed to benefit PPV replication by real-time fluorescence quantitative PCR and determination of median tissue culture infective dose(TCID). For the first time, the complex interaction between PPV infection and autophagy was explored in this study. It indicated that PPV could induce autophagy in ST cells, which in turn facilitated its own replication, which might be one of the mechanisms of the virus infection. These findings could facilitate the study of the pathogenesis of PPV infection and provide new insight into the development of effective therapeutic strategies.展开更多
Japanese encephalitis virus（JEV） is a significant causative agent of arthropod-borne encephalitis and what is less clear that the factors cause the virus wide spread. The objective was to confirm whether the homolog...Japanese encephalitis virus（JEV） is a significant causative agent of arthropod-borne encephalitis and what is less clear that the factors cause the virus wide spread. The objective was to confirm whether the homologous recombination imposed on JEV. The phylogenetic and homologous recombination analyses were performed based on 163 complete JEV genomes which were recently isolated. They were still separated into five genotypes（GI-GV） and the most of recently isolated JEVs were GI rather than GIII in Asian areas including China's Mainland. Two recombinant events were identified in JEV and the evidence of the recombination was observed between China and Japan isolates that partitioned into two distinct subclades, but still the same genotype（GIII）. Our data further suggested that most of the nucleotides in JEV genome were under negative selection; however, changes within codon 2 316（amino acid NS4b-44） showed an evidence of the positive selection.展开更多
该工程对安徽省某退役地块污染土壤进行修复治理,针对455.5 m 3六价铬、铅复合污染土壤,采用还原稳定化和固化稳定化的修复技术;针对1652.4 m 3总石油烃C 10-C 40、砷复合污染土壤,采用化学氧化和固化稳定化技术;针对8515.4 m 3单一六...该工程对安徽省某退役地块污染土壤进行修复治理,针对455.5 m 3六价铬、铅复合污染土壤,采用还原稳定化和固化稳定化的修复技术;针对1652.4 m 3总石油烃C 10-C 40、砷复合污染土壤,采用化学氧化和固化稳定化技术;针对8515.4 m 3单一六价铬污染土壤,选用还原稳定化方法;针对3745.8m 3单一石油烃C 10-C 40污染土壤,选用化学氧化方法。共计处置污染土壤14369 m 3,修复合格后进行异地阻隔封存。本项目的成功实施能够为其他类似污染场地土壤修复项目提供参考。展开更多
基金Supported by the National Natural Science Foundation of China(31372438,31201911)
文摘Porcine parvovirus(PPV) is one of the major causes of reproductive failure in pigs, which poses a great threat to the pig breeding industry and results in tremendous economic losses worldwide. Autophagy is the biological process of cell self-defense and self-protection. Despite many viruses can cause cell autophagy, when they enter cell or copied, the relationship between autophagy and PPV infection has not been reported. In this study, impact of autophagy after swine testicular(ST) cells infected by PPV was studied. Autophagy was demonstrated by the effective replication of PPV through transmission electron microscopy, immunofluorescence and western blot analysis. Moreover, autophagy was confirmed to benefit PPV replication by real-time fluorescence quantitative PCR and determination of median tissue culture infective dose(TCID). For the first time, the complex interaction between PPV infection and autophagy was explored in this study. It indicated that PPV could induce autophagy in ST cells, which in turn facilitated its own replication, which might be one of the mechanisms of the virus infection. These findings could facilitate the study of the pathogenesis of PPV infection and provide new insight into the development of effective therapeutic strategies.
基金Supported by the National Natural Science Foundation of China(31272569,31270187)Projects in the National Science&Technology Pillar Program during 12th Five-year Plan Period(2013BAD12B04)Harbin Science and Technology Bureau(RC2012XK002003)
文摘Japanese encephalitis virus（JEV） is a significant causative agent of arthropod-borne encephalitis and what is less clear that the factors cause the virus wide spread. The objective was to confirm whether the homologous recombination imposed on JEV. The phylogenetic and homologous recombination analyses were performed based on 163 complete JEV genomes which were recently isolated. They were still separated into five genotypes（GI-GV） and the most of recently isolated JEVs were GI rather than GIII in Asian areas including China's Mainland. Two recombinant events were identified in JEV and the evidence of the recombination was observed between China and Japan isolates that partitioned into two distinct subclades, but still the same genotype（GIII）. Our data further suggested that most of the nucleotides in JEV genome were under negative selection; however, changes within codon 2 316（amino acid NS4b-44） showed an evidence of the positive selection.
文摘该工程对安徽省某退役地块污染土壤进行修复治理,针对455.5 m 3六价铬、铅复合污染土壤,采用还原稳定化和固化稳定化的修复技术;针对1652.4 m 3总石油烃C 10-C 40、砷复合污染土壤,采用化学氧化和固化稳定化技术;针对8515.4 m 3单一六价铬污染土壤,选用还原稳定化方法;针对3745.8m 3单一石油烃C 10-C 40污染土壤,选用化学氧化方法。共计处置污染土壤14369 m 3,修复合格后进行异地阻隔封存。本项目的成功实施能够为其他类似污染场地土壤修复项目提供参考。
