Objective:To investigate the contamination and distribution of Vibrio parahaemolyticus and Vibrio alginolyticus in seafood in Haikou City.Methods:Three types of seafood sold in Haikou from 2020 to 2022 were collected,...Objective:To investigate the contamination and distribution of Vibrio parahaemolyticus and Vibrio alginolyticus in seafood in Haikou City.Methods:Three types of seafood sold in Haikou from 2020 to 2022 were collected,Vibrio parahaemolyticus and Vibrio alginolyticus were detected according to the National Food Safety Standard Food Microbiological Examination of Vibrio parahaemolyticus(GB 4789.7-2013),and they were identified by real-time fluorescence PCR.The detection of Vibrio parahaemolyticus and Vibrio alginolyticus in different kinds of seafood,different years and different quarters was analyzed.Results:A total of 119 seafood samples were collected.Among them,24 samples were positive with Vibrio parahaemolyticus,with a positive rate of 20.1%;46 samples were positive with Vibrio alginolyticus,with a positive rate of 38.7%.Among various types of seafood,shrimp have the highest positivity rate for Vibrio parahaemolyticus at 50%,while shellfish have the highest positivity rate for Vibrio alginolyticus at 48%.Comparing between monitoring years,the positive rate of Vibrio alginolyticus was the highest in 2021(76.7%),while the positive rate of Vibrio parahaemolyticus was the highest in 2022(25%).Comparing between different quarters,the positivity rate for Vibrio alginolyticus was found to be highest in the second quarter at 80%,while the positivity rate for Vibrio parahaemolyticus was highest in the fourth quarter at 33.3%.There were statistically significant differences(P<0.05)in the positivity rate for Vibrio alginolyticus in different years and quarters,as well as in the positivity rate for Vibrio parahaemolyticus in different types and quarters.Conclusion:Vibrio parahaemolyticus and Vibrio alginolyticus were found in seafood products in Haikou City from 2020 to 2022.It is recommended that relevant departments strengthen supervision to ensure the safety of seafood products consumed by the public.展开更多
人即刻早期反应基因2(immediate early response 2,IER2)可能参与肿瘤的发生发展,但它在此过程中的作用及表达调控的机制仍不清楚。本研究通过在线数据库GEPIA2与Kaplan-Meier Plotter分析发现:TNF和IER2基因在食管癌组织和宫颈癌组织...人即刻早期反应基因2(immediate early response 2,IER2)可能参与肿瘤的发生发展,但它在此过程中的作用及表达调控的机制仍不清楚。本研究通过在线数据库GEPIA2与Kaplan-Meier Plotter分析发现:TNF和IER2基因在食管癌组织和宫颈癌组织中高表达。而且IER2基因高表达的食管癌病人的预后显著较差。通过UCSC基因组数据库分析发现,在宫颈癌细胞和淋巴细胞内,TNFα(由TNF基因编码)诱导激活NF-κB结合IER2基因区。qRT-PCR检测证明,在食管癌和宫颈癌等细胞内,TNFα诱导NF-κB在转录水平上调IER2基因的表达。本文据此提出,TNFα诱导NF-κB上调IER2基因的表达可能是其在食管癌和宫颈癌等肿瘤中高表达的调控机制。本研究还通过在线数据库GeneMANIA和String分析发现11个与IER2具有关联性的基因。通过在线数据库Oncomine分析发现了88个基因在食管癌细胞内与IER2基因共表达。其中,包含7个与IER2具有关联性的基因ATF3、DUSP1、EGR1、EGR2、FOSB、JUN、NR4A1。本研究通过Oncomine数据库分析证明,在前列腺癌组织细胞内,这7个基因也与IER2共表达。并通过qRT-PCR检测验证这7个基因在食管癌细胞内与IER2基因共表达。GO注释表明,IER2可能通过与ATF3、EGR1、JUN相互作用,协同参与细胞增殖等生物学过程。CCK8检测证明,IER2基因过表达促进食管癌细胞生长增殖。总之,本研究初步揭示了IER2在食管癌和宫颈癌中高表达的调控机制及其作用,为深入研究该基因功能提供了线索。展开更多
基金Major Science and Technology Project of Hainan Province(No.ZDKJ202003)。
文摘Objective:To investigate the contamination and distribution of Vibrio parahaemolyticus and Vibrio alginolyticus in seafood in Haikou City.Methods:Three types of seafood sold in Haikou from 2020 to 2022 were collected,Vibrio parahaemolyticus and Vibrio alginolyticus were detected according to the National Food Safety Standard Food Microbiological Examination of Vibrio parahaemolyticus(GB 4789.7-2013),and they were identified by real-time fluorescence PCR.The detection of Vibrio parahaemolyticus and Vibrio alginolyticus in different kinds of seafood,different years and different quarters was analyzed.Results:A total of 119 seafood samples were collected.Among them,24 samples were positive with Vibrio parahaemolyticus,with a positive rate of 20.1%;46 samples were positive with Vibrio alginolyticus,with a positive rate of 38.7%.Among various types of seafood,shrimp have the highest positivity rate for Vibrio parahaemolyticus at 50%,while shellfish have the highest positivity rate for Vibrio alginolyticus at 48%.Comparing between monitoring years,the positive rate of Vibrio alginolyticus was the highest in 2021(76.7%),while the positive rate of Vibrio parahaemolyticus was the highest in 2022(25%).Comparing between different quarters,the positivity rate for Vibrio alginolyticus was found to be highest in the second quarter at 80%,while the positivity rate for Vibrio parahaemolyticus was highest in the fourth quarter at 33.3%.There were statistically significant differences(P<0.05)in the positivity rate for Vibrio alginolyticus in different years and quarters,as well as in the positivity rate for Vibrio parahaemolyticus in different types and quarters.Conclusion:Vibrio parahaemolyticus and Vibrio alginolyticus were found in seafood products in Haikou City from 2020 to 2022.It is recommended that relevant departments strengthen supervision to ensure the safety of seafood products consumed by the public.
文摘人即刻早期反应基因2(immediate early response 2,IER2)可能参与肿瘤的发生发展,但它在此过程中的作用及表达调控的机制仍不清楚。本研究通过在线数据库GEPIA2与Kaplan-Meier Plotter分析发现:TNF和IER2基因在食管癌组织和宫颈癌组织中高表达。而且IER2基因高表达的食管癌病人的预后显著较差。通过UCSC基因组数据库分析发现,在宫颈癌细胞和淋巴细胞内,TNFα(由TNF基因编码)诱导激活NF-κB结合IER2基因区。qRT-PCR检测证明,在食管癌和宫颈癌等细胞内,TNFα诱导NF-κB在转录水平上调IER2基因的表达。本文据此提出,TNFα诱导NF-κB上调IER2基因的表达可能是其在食管癌和宫颈癌等肿瘤中高表达的调控机制。本研究还通过在线数据库GeneMANIA和String分析发现11个与IER2具有关联性的基因。通过在线数据库Oncomine分析发现了88个基因在食管癌细胞内与IER2基因共表达。其中,包含7个与IER2具有关联性的基因ATF3、DUSP1、EGR1、EGR2、FOSB、JUN、NR4A1。本研究通过Oncomine数据库分析证明,在前列腺癌组织细胞内,这7个基因也与IER2共表达。并通过qRT-PCR检测验证这7个基因在食管癌细胞内与IER2基因共表达。GO注释表明,IER2可能通过与ATF3、EGR1、JUN相互作用,协同参与细胞增殖等生物学过程。CCK8检测证明,IER2基因过表达促进食管癌细胞生长增殖。总之,本研究初步揭示了IER2在食管癌和宫颈癌中高表达的调控机制及其作用,为深入研究该基因功能提供了线索。
