The aim of this study is to screen proteins interacting with interferon-α （IFN-α）. The IFN-α gene was amplified by polymerase chain reaction （PCR） and cloned into pGBKT-/vector, then the resulted pGBKT-/-IFN-α...The aim of this study is to screen proteins interacting with interferon-α （IFN-α）. The IFN-α gene was amplified by polymerase chain reaction （PCR） and cloned into pGBKT-/vector, then the resulted pGBKT-/-IFN-α vector was transformed into yeast strain AH109. The transformed yeast AH109 was mated with yeast Y187 containing liver cDNA library plasmid in 2 × YPDA medium. Diploid yeast was plated on synthetic dropout nutrient medium （SD/-Trp-Leu-His-Ade and SD/-Trp-Leu-His） con- taining X-α-gal for selection. After plasmid extraction and enzyme cutting analysis, the blue colonies were subjected to sequence analysis and the results were analyzed by bioinformatics. The results showed that IFN-α was successful cloned into the pGBKT7 vector. IFN-α was expressed and there was no selfactivation and toxicity in AH109. Thirty-four positive colonies were obtained after yeast-two hybrid technique screening. After sequence analysis, eight clones were found to have a binding effect with IFN-α protein. IFN-α was successfully cloned into the pGBKT7 vector. IFN-α protein was expressed and there was no self-activation and toxicity in AH109. Eight proteins that interacted with IFN-α, including vitronectin, fibrinogen A alpha polypeptide, HIV-1 Tat interactive protein 2, arginase, NADH dehydrogenase 1 beta subcomplex, transferrin receptor 2 alpha （TFR2）, HCC-1, alcohol dehydrogenase IB （ADHIB） have been identified as IFN-α-binding proteins.展开更多
A blue light-emitting material, 2-（N-ethyl-anthraceneyl-9） imidazo [4,5-f] 1,10-phenanthroline （EAIP）, has been synthesized and characterized by 1H NMR, IR, elemental and single crystal X-ray diffraction analysis....A blue light-emitting material, 2-（N-ethyl-anthraceneyl-9） imidazo [4,5-f] 1,10-phenanthroline （EAIP）, has been synthesized and characterized by 1H NMR, IR, elemental and single crystal X-ray diffraction analysis. The results of theoretical calculations indicate that the HOMO and LUMO distributions mairdy locate at the anthracene fragment. It displays bright blue emission in both solid state and dichloromethane solution. The emission quantum yield calculated is 0.76. 2009 Bin Li. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.展开更多
A new europium（Ⅲ） complex, tris（dibertzoylmethanate）{ 1-[9-hexyl-9-carbazole]-2-（2-pyridyl）-bertzimidazole}europium（Ⅲ） [Eu（DBM）a（CPyBM）] was synthesized and used as an electron-acceptor and electron-tran...A new europium（Ⅲ） complex, tris（dibertzoylmethanate）{ 1-[9-hexyl-9-carbazole]-2-（2-pyridyl）-bertzimidazole}europium（Ⅲ） [Eu（DBM）a（CPyBM）] was synthesized and used as an electron-acceptor and electron-transport layer in organic photovoltaic （PV） device. Power conversion efficiency achieved from the device was 1.04% under illumination with 365 nm UV light at 1.6 mW/cm^2. Compared with the previous reported devices based on Eu（Ⅲ） complexes, the PV performances were improved. The working mechanism of the organic PV device was discussed.展开更多
文摘The aim of this study is to screen proteins interacting with interferon-α （IFN-α）. The IFN-α gene was amplified by polymerase chain reaction （PCR） and cloned into pGBKT-/vector, then the resulted pGBKT-/-IFN-α vector was transformed into yeast strain AH109. The transformed yeast AH109 was mated with yeast Y187 containing liver cDNA library plasmid in 2 × YPDA medium. Diploid yeast was plated on synthetic dropout nutrient medium （SD/-Trp-Leu-His-Ade and SD/-Trp-Leu-His） con- taining X-α-gal for selection. After plasmid extraction and enzyme cutting analysis, the blue colonies were subjected to sequence analysis and the results were analyzed by bioinformatics. The results showed that IFN-α was successful cloned into the pGBKT7 vector. IFN-α was expressed and there was no selfactivation and toxicity in AH109. Thirty-four positive colonies were obtained after yeast-two hybrid technique screening. After sequence analysis, eight clones were found to have a binding effect with IFN-α protein. IFN-α was successfully cloned into the pGBKT7 vector. IFN-α protein was expressed and there was no self-activation and toxicity in AH109. Eight proteins that interacted with IFN-α, including vitronectin, fibrinogen A alpha polypeptide, HIV-1 Tat interactive protein 2, arginase, NADH dehydrogenase 1 beta subcomplex, transferrin receptor 2 alpha （TFR2）, HCC-1, alcohol dehydrogenase IB （ADHIB） have been identified as IFN-α-binding proteins.
基金the financial supports of One Hundred Talents Project from the Chinese Academy of Sciences and the NSFC(No.50872130)
文摘A blue light-emitting material, 2-（N-ethyl-anthraceneyl-9） imidazo [4,5-f] 1,10-phenanthroline （EAIP）, has been synthesized and characterized by 1H NMR, IR, elemental and single crystal X-ray diffraction analysis. The results of theoretical calculations indicate that the HOMO and LUMO distributions mairdy locate at the anthracene fragment. It displays bright blue emission in both solid state and dichloromethane solution. The emission quantum yield calculated is 0.76. 2009 Bin Li. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.
基金The authors gratefully thank the financial supports of 0ne Hundred Talents Project from Chinese Academy of Sciencesthe National Natural Science Foundations of China(No.20571071).
文摘A new europium（Ⅲ） complex, tris（dibertzoylmethanate）{ 1-[9-hexyl-9-carbazole]-2-（2-pyridyl）-bertzimidazole}europium（Ⅲ） [Eu（DBM）a（CPyBM）] was synthesized and used as an electron-acceptor and electron-transport layer in organic photovoltaic （PV） device. Power conversion efficiency achieved from the device was 1.04% under illumination with 365 nm UV light at 1.6 mW/cm^2. Compared with the previous reported devices based on Eu（Ⅲ） complexes, the PV performances were improved. The working mechanism of the organic PV device was discussed.
