Retrotransposons are present in all plant genomes and play important roles in genome size,genome structure remodeling,gene function and genome evolution.Me07Em02 marked by sequence-related amplified polymorphism (SRAP...Retrotransposons are present in all plant genomes and play important roles in genome size,genome structure remodeling,gene function and genome evolution.Me07Em02 marked by sequence-related amplified polymorphism (SRAP) is verified,recovered,sequenced and analyzed,which closely link to the flesh color around the stone.By comparison with the peach genome Peach v1.0,a part of the sequence of copia-like retrotransposons is obtained.With the aid of DANMAN and DNASTAR sequence analysis software,BLASTn alignment is carried out in the peach genome database and GenBank.The whole genome sequence of the copialike retrotransposon in the peach is located at 9 939 764~9 944 771 bp in Scaffold-1 of Peach v1.0,with a total length of 5 008 bp.The LTR on both sides is exactly the same,the length is 444 bp,and the largest ORF box is located at the 487~4 545 bp of the sequence,encoding 1 535 amino acids.The amino acid sequence was submitted to GenBank for Protein BLAST alignment,The results showed that this sequence also has the typical amino acid sequence characteristics of the copia-like retrotransposon.At the same time,the method of peach retrotransposon labeling is preliminarily established,and the genetic diversity of the peach is analyzed.展开更多
文摘Retrotransposons are present in all plant genomes and play important roles in genome size,genome structure remodeling,gene function and genome evolution.Me07Em02 marked by sequence-related amplified polymorphism (SRAP) is verified,recovered,sequenced and analyzed,which closely link to the flesh color around the stone.By comparison with the peach genome Peach v1.0,a part of the sequence of copia-like retrotransposons is obtained.With the aid of DANMAN and DNASTAR sequence analysis software,BLASTn alignment is carried out in the peach genome database and GenBank.The whole genome sequence of the copialike retrotransposon in the peach is located at 9 939 764~9 944 771 bp in Scaffold-1 of Peach v1.0,with a total length of 5 008 bp.The LTR on both sides is exactly the same,the length is 444 bp,and the largest ORF box is located at the 487~4 545 bp of the sequence,encoding 1 535 amino acids.The amino acid sequence was submitted to GenBank for Protein BLAST alignment,The results showed that this sequence also has the typical amino acid sequence characteristics of the copia-like retrotransposon.At the same time,the method of peach retrotransposon labeling is preliminarily established,and the genetic diversity of the peach is analyzed.