为了建立高效、灵敏的猪流行性腹泻病毒(PEDV)检测方法,本研究从GenBank数据库中获取PEDV N基因序列,扩增出PEDV N基因标准质粒,并在N基因的保守区域内设计了一对特异性荧光定量引物,成功建立了SYBR Green I实时荧光定量PCR检测方法。...为了建立高效、灵敏的猪流行性腹泻病毒(PEDV)检测方法,本研究从GenBank数据库中获取PEDV N基因序列,扩增出PEDV N基因标准质粒,并在N基因的保守区域内设计了一对特异性荧光定量引物,成功建立了SYBR Green I实时荧光定量PCR检测方法。经过一系列试验表明,该检测方法线性关系良好,R^(2)值为0.99;特异性强,敏感性高,最低可检测至2.23 copies/μL,比普通PCR灵敏约100倍;重复性好,组内变异系数为0.25%~0.43%,组间变异系数为0.67%~0.97%;对于各地区96份临床样品检测出PEDV阳性率为25%。本研究建立的实时荧光定量PCR检测方法为PEDV的临床诊断、流行病学调查以及定量研究提供了有效的检测工具。展开更多
Using ground and remote sensing monitoring, and national standards of snow disaster monitoring evaluation standards, quantitative evaluation of snow disaster was realized. Threshold of animal husbandry weather forecas...Using ground and remote sensing monitoring, and national standards of snow disaster monitoring evaluation standards, quantitative evaluation of snow disaster was realized. Threshold of animal husbandry weather forecast indexes were applied to establish snow disaster early warning for different grasslands in pasturing areas of Inner Mongolia, and make the grade distribution map of snow disaster early warning. The forecast results basically met the real conditions, it proved that this forecast method was capable of evaluating scale and influence degree of snow disaster. However, the snow disaster grade forecast deviated from the real conditions for the influence of weather forecast accuracy rate.展开更多
基金Sponsored by Scientific and Technological Innovation Program of the Inner Mongolia Meteorological Bureau(nmgqxkjcx201115)
文摘Using ground and remote sensing monitoring, and national standards of snow disaster monitoring evaluation standards, quantitative evaluation of snow disaster was realized. Threshold of animal husbandry weather forecast indexes were applied to establish snow disaster early warning for different grasslands in pasturing areas of Inner Mongolia, and make the grade distribution map of snow disaster early warning. The forecast results basically met the real conditions, it proved that this forecast method was capable of evaluating scale and influence degree of snow disaster. However, the snow disaster grade forecast deviated from the real conditions for the influence of weather forecast accuracy rate.